We narrowed to 65,914 results for: des.1

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pcDNA3-sACE2-WT(732)-IgG1

Plasmid

#154104

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pH-nCas9-PPE

Plasmid

#140447

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

Insert

nCas9(H840A)-M-MLV

Use

CRISPR

Expression

Plant

Mutation

H840A for Cas9; D200N, T306K, W313F, T330P and L…

Promoter

maize Ubiquitin-1, OsU3

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pZ149-pcDNA3-BhCas12b_v4

Plasmid

#122446

Purpose

Expresses BhCas12b_v4 in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

CMV-NLS-BhCas12b_v4-NLS-3xHA

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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CMV Flag ATRwt

Plasmid

#41909

Depositor

Stephen Elledge

Article

Cortez et al Science. 2001 Nov 23;294(5547):1713-6.

Insert

ATR ATR serine/threonine kinase (ATR Human)

Tags

FLAG

Expression

Mammalian

Promoter

SRa

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-hSynI-rtTAV16

Plasmid

#102367

Purpose

AAV vector to drive TET-ON activator expression from human synapsin I promoter

Depositor

Tetsuo Yamamori

Article

Watakabe et al Front Neural Circuits. 2014 Sep 17;8:110

Insert

rtTAV16

Use

AAV

Expression

Mammalian

Mutation

rtTA variant reported in Gene Therapy (2006) 13, …

Promoter

human synapsin I promoter

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-As

Plasmid

#86196

Purpose

AsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

AsCpf1 gRNA cloning site for ribozyme cleavage

Use

CRISPR

Tags

Hammerhead ribozyme and HDV ribozyme

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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hsgk1

Plasmid

#83432

Purpose

full length human sgk1 with Flag tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2008 Nov;295

Insert

human Sgk1 (SGK1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab11a

Plasmid

#79805

Purpose

Express TagBFP labeled human Rab11a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab11a (RAB11A Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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MSP1673

Plasmid

#65769

Purpose

Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7 (x2)

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBa-KIF5C 559-tdTomato-FKBP

Plasmid

#64211

Purpose

KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Kif5c (Kif5c Rat)

Tags

FKBP and tdTomato

Expression

Mammalian

Mutation

motor domain aa1-559

Promoter

chicken Beta Actin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-SUMO2-Q90P

Plasmid

#36448

Purpose

Expresses HA-Sumo2Q90P mutant in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO2 (SUMO2 Human)

Tags

HA

Expression

Mammalian

Mutation

Mutated Q90P

Promoter

T7

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-MTS-CA-c-Src-FLAG

Plasmid

#44654

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed Tyr 530 to Phe

Promoter

CMV promoter

Available Since

May 6, 2013

Availability

Academic Institutions and Nonprofits only

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mSIRT3-L

Plasmid

#33309

Depositor

Michael Sack

Article

Bao et al J Cell Biochem. 2010 May;110(1):238-47.

Insert

Sirt3 (Sirt3 Mouse)

Tags

3XFlag

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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plv-AcGFP-SOD1 (G93A)

Plasmid

#27142

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Tags

AcGFP

Mutation

G93A

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only
CMV-FLAG-Core-R (pMO29)

Plasmid

#24480

Depositor

Melanie Ott

Article

Schwer et al J Virol. 2004 Aug . 78(15):7958-68.

Insert

Core

Tags

FLAG

Expression

Mammalian

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-QF

Plasmid

#24339

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

QF

Tags

His and Myc

Expression

Mammalian

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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pJJDuet30

Plasmid

#11962

Depositor

Hideo Iwai

Article

Zuger et al Nat Biotechnol. 2005 Jun . 23(6):736-40.

Insert

Ssp DnaE-N

Tags

GB1

Expression

Bacterial

Available Since

Sept. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pGL-FLKif3B

Plasmid

#13743

Depositor

Linda Wordeman

Article

Ginkel et al Mol Biol Cell. 2000 Dec . 11(12):4143-58

Insert

mouse Kif3B (Kif3b Mouse)

Use

Cre/Lox

Tags

GFP

Expression

Mammalian

Available Since

Jan. 29, 2007

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-FIH1

Plasmid

#21403

Depositor

Eric Metzen

Article

Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

Insert

HIF1AN (HIF1AN Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pGL3 GATA Luc

Plasmid

#85695

Purpose

Luciferase reporter containing three repeats of the GATA consensus site

Depositor

Licio Collavin, Giannino Del Sal

Article

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

3xGATA and minimal MT promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

3xGATA and minimal MT promoter

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only