162,309 results

1. pDEST-SorLA-v2

   Plasmid

   #154900

   Purpose

   Expresses SorLA fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

   Depositor

   Johanna Ivaska

   Article

   Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

   Insert

   SORL1 (SORL1 Human)
   

   Use

   Tags

   Venus fragment 2 (v2)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWT35S-mCherry

   Plasmid

   #182582

   Purpose

   Transient expression of mCherry in plant cell (Cytosol)

   Depositor

   Yutaka Kodama

   Article

   Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. P3-dCas9-Tet1-GFP-Puro

   Plasmid

   #190728

   Purpose

   Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

   Depositor

   Maja Jagodic

   Article

   Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

   Insert

   dCas9-tet1
   

   Use

   CRISPR

   Tags

   3XFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Changed Pro434 codon from CCG to CCC and Gly468 f…

   Promoter

   pCAG

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CaV2.1/pcDNA3.1 ~ Human

   Plasmid

   #140575

   Purpose

   Expression in mammallian cells or oocytes

   Depositor

   Dan Minor

   Article

   Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

   Insert

   CaV2.1 Human (CACNA1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBluescript II SK--POLQ

   Plasmid

   #64873

   Purpose

   cloning vector for human POLQ

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Insert

   POLQ (POLQ Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDT-SuperFi-Cas9

   Plasmid

   #183630

   Purpose

   Expression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coli

   Depositor

   David Taylor

   Article

   Bravo et al Nature. 2022 Mar 2. pii: 10.1038/s41586-

   Insert

   SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …

   Promoter

   T7

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDA019

   Plasmid

   #131452

   Purpose

   control expression vector

   Depositor

   Kathleen Burns

   Article

   Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BoxB in pcDNA3

   Plasmid

   #29727

   Depositor

   Howard Chang

   Article

   Wang et al Nature. 2011 Apr 7;472(7341):120-4. doi:

   Insert

   5BoxB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSFDuet-1_VNp6-mNeongreen

   Plasmid

   #182388

   Purpose

   Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp6-mNeongreen-His
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiCRISPRv2-Opti

    Plasmid

    #163126

    Purpose

    LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB_SBE4:mScarlet-I-NLS

    Plasmid

    #154961

    Purpose

    encodes transcriptional reporter of SMAD2/3

    Depositor

    Joshua Knowles, Ewa Bielczyk-Maczynska

    Article

    Taylor et al Sci Rep. 2020 Aug 21;10(1):14084. doi: 1

    Insert

    mScarlet
    

    Use

    Piggybac

    Tags

    NLS-PEST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1.Gαi2-LgB91

    Plasmid

    #134358

    Purpose

    Nanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαi2-LgB91 (GNAI2 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-puro-SNAP-FLAG-CD59

    Plasmid

    #50374

    Purpose

    Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    SNAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SR alpha

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-P4M-SidM

    Plasmid

    #51471

    Purpose

    Biosensor for PtdIns4P

    Depositor

    Tamas Balla

    Article

    Hammond et al J Cell Biol. 2014 Apr 7.

    Insert

    P4M-SidM(546-647)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a_MBP_RBFOX2

    Plasmid

    #224292

    Purpose

    MBP-RBFOX2 protein purification from bacteria

    Depositor

    Chuan He

    Article

    Dou et al Nat Cell Biol. 2023 Sep;25(9):1359-1368.

    Insert

    RNA binding fox-1 homolog 2 (RBFOX2 Human)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCI NL4-3 Nef-HA-WT

    Plasmid

    #24162

    Depositor

    Warner Greene

    Article

    Geleziunas et al Nature. 2001 Apr 12. 410(6830):834-8.

    Insert

    Nef (nef HIV)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pDR121

    Plasmid

    #37148

    Depositor

    Dale Ramsden

    Insert

    XRCC6 (XRCC6 Human)
    

    Use

    Tags

    H6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

18. AbVec2.1-mIglc2

    Plasmid

    #127156

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), lambda 2 constant region

    Depositor

    Hedda Wardemann

    Article

    Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

    Insert

    Iglc2 (Iglc2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. hGli3 flag3x

    Plasmid

    #84921

    Purpose

    mammalian expression of Gli3

    Depositor

    Martin Fernandez-Zapico

    Insert

    Gli3 (GLI3 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Anti-6xHis [N144/14R]

    Plasmid

    #177500

    Purpose

    Mammalian Expression Plasmid of anti-6xHis. Derived from hybridoma N144/14.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-6xHis recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

    Plasmid

    #207878

    Purpose

    AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

    Depositor

    Yeh-Hsing Lao

    Article

    Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MAC_C_CH60

    Plasmid

    #111662

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    CH60 (HSPD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1_sfCherry2(1-10)

    Plasmid

    #82602

    Purpose

    Expresses sfCherry2(1-10) fusion in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Glu 118 to Gln, changed Thr 128 to Ile

    Promoter

    CMV

    Available since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Lifeact-7-iRFP670

    Plasmid

    #103032

    Purpose

    Expression of iRFP670-tagged Lifeact with G418 selection in mammalian cells

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCG_SARS-CoV2 M C-V5-tag

    Plasmid

    #179978

    Purpose

    Mammalian expression vector for SARS-CoV-2 M, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-2 M (M Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKS diaCas9_sgRNA

    Plasmid

    #74923

    Purpose

    Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter

    Depositor

    Per Winge

    Article

    Nymark et al Sci Rep. 2016 Apr 25;6:24951. doi: 10.10

    Inserts

    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Cas9 module and sgRNA module

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-29b(+)_His-CBD-3C-EmPNGaseF

    Plasmid

    #218193

    Purpose

    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseF

    Depositor

    Florence Pojer

    Insert

    PNGaseF
    

    Use

    Tags

    tufA-His-CBD-3C

    Expression

    Bacterial

    Mutation

    codon optimized Ecoli, residues 41-354

    Promoter

    T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP(C1)-PP1alpha

    Plasmid

    #44224

    Depositor

    Angus Lamond, Laura Trinkle-Mulcahy

    Article

    Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

    Insert

    PP1A (PPP1CA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. lenti SYN-dCas9-KRAB-MeCP2

    Plasmid

    #155365

    Purpose

    Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

    Depositor

    Jeremy Day

    Article

    Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    3x FLAG and 7x His

    Expression

    Mammalian

    Mutation

    Promoter

    SYN

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-RSV-SpCas9

    Plasmid

    #85450

    Purpose

    Express SpCas9 in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pRSV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pRSV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge