We narrowed to 10,632 results for: t7

1. pET29b_Qcat4

   Plasmid

   #45132

   Depositor

   Christopher Petzold

   Article

   Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

   Insert

   E-coli-pathways-QconCAT4
   

   Tags

   6XHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET29b_Qcat17

   Plasmid

   #45145

   Depositor

   Christopher Petzold

   Article

   Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

   Insert

   E-coli-pathways-QconCAT17
   

   Tags

   6XHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. S019

   Plasmid

   #174552

   Purpose

   pET-iBclxL-v3, His tag for affinity purification

   Depositor

   Bruno Correia

   Article

   Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

   Insert

   iBclxL-v3, Bclxl inhibitor resistant protein
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. S330

   Plasmid

   #174553

   Purpose

   pET-iBcl2-v4, His tag for affinity purification

   Depositor

   Bruno Correia

   Article

   Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

   Insert

   ibcl2-v4, bcl2 inhibitor resistant protein
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-28a-His6-SUMO-VAMP8

   Plasmid

   #135553

   Purpose

   Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP8
   

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. TAL2288

   Plasmid

   #36723

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   FANCG-TALEN_Left (Fancg Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pK RagA-RagC

   Plasmid

   #99649

   Purpose

   Bacterial expression of wildtype Rag GTPase dimer

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   10x His tag, Poly arginine linker, and SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Zebrafish-gRNA-0007

   Plasmid

   #42247

   Purpose

   gRNA targeted to zebrafish gene th1

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   gRNA-th1 (th Zebrafish)
   

   Use

   CRISPR; Zebrafish expression

   Promoter

   T7

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

9. Calprotectin S100A9 toehold switch sensor

   Plasmid

   #110717

   Purpose

   Toehold switch sensor to detect Calprotectin S100A9 mRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   Calprotectin S100A9 toehold switch sensor
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. D. vulgaris Cas5c/pHMGWA

    Plasmid

    #81182

    Purpose

    Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Cas5c
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. D. vulgaris Cas8c/pHMGWA

    Plasmid

    #81183

    Purpose

    Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Cas8c
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TAL2306

    Plasmid

    #36734

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    ERCC2-TALEN_Left (Ercc2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

13. TAL2254

    Plasmid

    #36697

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FANCC-TALEN_Left (Fancc Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pACYC-Skp-hLYZ

    Plasmid

    #83924

    Purpose

    Co-expresses cytoplasmic copies of the Skp protein folding chaperone and wild type human lysozyme..

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Skp
    lysozyme (LYZ Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCellFree_G06_ZIKV NS2A

    Plasmid

    #145353

    Purpose

    Cell-free expression vector for Zika virus NS2A protein with C-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS2A
    

    Tags

    mCherry and His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. TAL3179

    Plasmid

    #41353

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-sox2-Right (sox2 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

17. HOXA9 (murine) HIS-tag pET

    Plasmid

    #8492

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HoxA9 (Hoxa9 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    BamHI/BalI fusion into amino acid #2 of HOXA9; ma…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pET15b/GgVcl V1-851 (aka Vh)

    Plasmid

    #46172

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

    Insert

    Vcl 1-851 (VCL Chicken)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 1-851 (aka Vh) head domain

    Promoter

    T7

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TAL2314

    Plasmid

    #36740

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FANCF-TALEN_Left (Fancf Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

20. TAL2322

    Plasmid

    #36748

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FOXO1-TALEN_Left (FOXO1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only