163,570 results

1. pBE14

   Plasmid

   #174653

   Purpose

   In vivo Cre-lox circularization in E. coli

   Depositor

   Huimin Zhao

   Article

   Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

   Insert

   Cre
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF1027

   Plasmid

   #141784

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RARA (RARA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX458-Dual-Guide-Donor-ShuttleOnly

   Plasmid

   #171098

   Purpose

   Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   XbaI site added upstream of U6 promotor. GtoC mu…

   Promoter

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA5/FRT/TO_H2B_HaloTag7

   Plasmid

   #169329

   Purpose

   Expression of H2B-HaloTag7 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag7 (H2BC21 Human)
   

   Use

   Tags

   HaloTag7

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AARS_C2_pNIC-Bio3

   Plasmid

   #153047

   Purpose

   Expression construct for producing Avi tagged Alanyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   AARS (AARS Human)
   

   Use

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1

   Plasmid

   #193942

   Purpose

   a fully murine CAR targeting CD105 (endoglin), a component of the TGFβ receptor expressed on the surface of certain solid tumors and acute leukemias.

   Depositor

   Greg Delgoffe

   Article

   Lontos et al Oncoimmunology. 2022 Oct 18;11(1):213122

   Insert

   heavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)

   Plasmid

   #154824

   Purpose

   (eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegans

   Depositor

   Patrick Phillips

   Article

   Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

   Insert

   Peft-3::wrmScarlet::tbb-2 UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   eef-1A.1 (eft-3)

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWXC078

   Plasmid

   #222961

   Purpose

   C. elegans codon-optimized mScarlet-I3 with 3 introns cloned into pPD95.75 backbone

   Depositor

   Oliver Hobert

   Article

   Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

   Insert

   mScarlet-I3
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLifeAct-mTurquoise2

   Plasmid

   #36201

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   LifeAct
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMET7-GAG-EGFP

    Plasmid

    #80605

    Purpose

    Mammalian expression vector that expresses HIV-1 GAG with EGFP fused to it

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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