We narrowed to 2,418 results for: EFS
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Plasmid#108104PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation)PromoterEFS promoterAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG NC
Plasmid#214675PurposeNegative control plasmid for splicing reporters consisting of MCP fused FLAG epitope tagsDepositorInsertFLAG
TagsMCP and V5ExpressionMammalianPromoterEF-1aAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PE2max-BSD
Plasmid#191102PurposeLentiviral expression plasmid of PEmax prime editor with P2A-BSD markerDepositorInsertPEmax-P2A-BSD
UseCRISPR and LentiviralExpressionMammalianMutationChanged R221K, N394K, H840A from SpCas9PromoterEF-1aAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
5HRE/GFP
Plasmid#46926Purposehypoxia-responsive enhanced green fluorescent protein (EGFP)-based systemDepositorInsert5X HRE of VEGF (VEGFA Human)
Tagsd2EGFPExpressionMammalianMutationfive copies of a 35-bp fragment from the hypoxia-…PromoterEF-1α promoterAvailable SinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo
Plasmid#139449PurposeLentiviral vector with gRNA scaffold and neomycin selectable markerDepositorInsertno sgRNA inserted; resistance gene: neoR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-218-5p
Plasmid#103357PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-218-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-218-5p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
FH-TET3-pEF
Plasmid#49446PurposeThis plasmid expresses full-length human TET3.DepositorInsertTet methylcytosine dioxygenase 3 (TET3 Human)
TagsFlag and HAExpressionMammalianPromoterEF-1alphaAvailable SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
FH-TET3-HxDmut-pEF
Plasmid#127896PurposeExpresses ten-eleven-translocation 3 (TET3) containing HxD mutation at H1077Y and D1079ADepositorAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL-3F-BirA
Plasmid#220147PurposeExpresses VHL-BirA for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationcodon optimizedPromoterEF-1aAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
v1em-Nterm-PE2max-U6-sgRNA
Plasmid#198732PurposeAAV genome encoding N-terminal PE2max and U6 expression cassetteDepositorInsertNterm PE2max-NpuN
UseAAVPromoterEFSAvailable SinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB9v1
Plasmid#210028PurposeContains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puroDepositorInsertsCas9
rtta-T2A-puro
UseCRISPRTagsSV40 NLS and nucleoplasmin NLSExpressionMammalianPromoterEF-1α and TRE3GAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-130b-3p
Plasmid#103217PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-130b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-130b-3p target (MIR130B Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-3074-5p
Plasmid#103408PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-3074-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-3074-5p target (MIR3074 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-30d-5p
Plasmid#103417PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30d-5p target (MIR30D Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-99b-5p
Plasmid#103767PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-99b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-99b-5p target (MIR99B Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-30a-5p
Plasmid#103410PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30a-5p target (MIR30A Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-eCas9
Plasmid#140237PurposeLentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffoldDepositorTypeEmpty backboneUseCRISPR and LentiviralTags3xFLAGExpressionMammalianPromoterEF-1a coreAvailable SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PE2-BSD
Plasmid#161514PurposeExpression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selectionDepositorInsertPE2-P2A-BSD
UseLentiviralTagsSV40 NLSExpressionMammalianPromoterEF-1α core promoterAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-GalNAc-T2-msGFP2
Plasmid#140164PurposeExpresses the Golgi marker GalnacT2-msGFP2DepositorAvailable SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7a-5p
Plasmid#103146PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7a-5p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-NRCH-PE2max-BSD
Plasmid#191103PurposeLentiviral expression plasmid of NRCH-PEmax prime editor with P2A-BSD markerDepositorInsertNRCH-PEmax
UseCRISPR and LentiviralExpressionMammalianMutationChanged R221K, N394K, H840A from SpCas9-NRCHPromoterEF-1aAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7f-5p
Plasmid#103156PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7f-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7f-5p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF1alpha-rtTA-Antares
Plasmid#197587PurposeExpresses TET-On reverse transactivator labeled with AntaresDepositorInsertrtTA-P2A-Antares
TagsAntaresExpressionMammalianPromoterEF-1alphaAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRpuro-sgROSA26
Plasmid#231562PurposepLentiCRISPR expression vector for Cas9 and gRNA targeting human ROSA26 locus (Puromycin selection marker)DepositorInsertsgROSA26
UseCRISPR and LentiviralExpressionMammalianPromoterU6, EF-1aAvailable SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB1163
Plasmid#210032PurposeContains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blastDepositorInsertsCas11-P2A-Cas6-T2A-Cas3
rtta-T2A-blast
UseCRISPRTagsSV40 NLSExpressionMammalianPromoterEF-1α and TRE3GAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-NG-PE2-BSD
Plasmid#176933PurposeExpresses PE2-NG variant in mammalian cellsDepositorInsertPE2-NG variant
UseLentiviralTagsSV40 NLSExpressionMammalianPromoterEFSAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
LVDP 2P.2
Plasmid#231904PurposeFor the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging firefly luciferase mRNA into the VLPsDepositorUseLentiviralMutationR203M in N proteinPromoterCMV, EF-1aAvailable SinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEBB COMMD3 Flag
Plasmid#74895PurposeExpress COMMD3 in mammalian cellsDepositorAvailable SinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF-ManII-GFP
Plasmid#160905PurposeExpresses the Golgi marker ManII-GFP in mammalian cellsDepositorAvailable SinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_Sik3
Plasmid#119972PurposeLentiviral expression plasmid of mouse Sik3 cDNA with neomycin resistance geneDepositorAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-9-5p
Plasmid#103749PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-9-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-9-5p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG/hTAZ-FL
Plasmid#139979PurposeExpresses full length human Tafazzin with codon modified for cloning. Transgene flanked by AAV2 ITRDepositorAvailable SinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB12A
Plasmid#210035PurposeContains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puroDepositorInsertsCas12a
rtta-T2A-puro
UseCRISPRTagsnucleoplasmin NLSExpressionMammalianPromoterEF-1α and TRE3GAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-199a-3p
Plasmid#103319PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-199a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-199a-3p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TERT K78E-IRES-Puro
Plasmid#182591Purposeexpresses TERT K78E in mammalian cellsDepositorAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBB COMMD1 Flag
Plasmid#74893PurposeExpress COMMD1 in mammalian cellsDepositorAvailable SinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-29b-3p
Plasmid#103394PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-29b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-29b-3p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-GFPg1 (BB09)
Plasmid#139461PurposeLentiviral vector with gRNA targeting GFP; includes puromycin selectable markerDepositorInsertGFP-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-181a-5p
Plasmid#103276PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-181a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-181a-5p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-133b
Plasmid#103223PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-133b target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-133b target (MIR133B Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only