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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 39,536 results for: Eras

Showing: 1201 - 1240 of 39536 results

  1. pRpr150-LUC

    Plasmid
    #85700
    Purpose
    luciferase reporter driven by a DNA damage responsive cis-regulatory sequence from the reaper locus, containing a p53 binding site (Rpr150 enhancer)
    Depositor
    Giannino Del Sal, Licio Collavin
    Article
    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-
    Insert
    RpR 150 enhancer and hsp70 minimal promoter
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    RpR 150 enhancer and hsp70 minimal promoter
    Available since
    March 21, 2017
    Availability
    Academic Institutions and Nonprofits only

  14. pcDNA3.1 NKX6-2(1-196)-Venus

    Plasmid
    #209182
    Purpose
    Mammalian expression of human NKX6-2 cDNA SPAX8-related truncated mutation containing the amino acids 1 to 196; fused with the green fluorescent protein Venus allowing the use of fluorescent based protocols.
    Depositor
    Federico Herrera
    Article
    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023
    Insert
    NKX6-2 (1-196) fused with Venus (NKX6-2 Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    209182-plasmid-map-sequence-id-413578

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  15. pcDNA3.1 NKX6-2(1-188)-Venus

    Plasmid
    #203714
    Purpose
    SPAX8-related truncated mutation (E189*) containing the amino acids 1 to 188 of human NKX6-2 fused with the Venus fluorescent protein
    Depositor
    Federico Herrera
    Article
    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023
    Insert
    NKX6-2 (1-188) fused with Venus (NKX6-2 Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    deletion of amino acids 189-277
    Promoter
    CMV
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    203714-plasmid-map-sequence-id-401198

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  16. pcDNA3.1 NKX6-2(1-40)-Venus

    Plasmid
    #203713
    Purpose
    SPAX8-related truncated mutation (K41*) containing the amino acids 1 to 40 of human NKX6-2, fused with the Venus fluorescent protein.
    Depositor
    Federico Herrera
    Article
    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023
    Insert
    NKX6-2 (1-40) fused with Venus (NKX6-2 Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    deletion of amino acids 41-277
    Promoter
    CMV
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    203713-plasmid-map-sequence-id-401045

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  24. pJK516

    Plasmid
    #71578
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glutamate-435 to glutamine
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    71578-plasmid-map-sequence-id-130937

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  25. pJK524

    Plasmid
    #71580
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes asparagine-347 to alanine
    Promoter
    T7
    Available since
    April 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    71580-plasmid-map-sequence-id-130939

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  26. pJK513

    Plasmid
    #71576
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu294>Ala mutant (AarCH6-E294A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glutamate-294 to alanine
    Promoter
    T7
    Available since
    April 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    71576-plasmid-map-sequence-id-130935

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  27. pJK564

    Plasmid
    #71581
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes serine-71 to alanine
    Promoter
    T7
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71581-plasmid-map-sequence-id-130940

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  28. pJK517

    Plasmid
    #71579
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glutamate-435 to aspartate
    Promoter
    T7
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71579-plasmid-map-sequence-id-130938

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  29. pJK514

    Plasmid
    #71577
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glutamine-435 to alanine
    Promoter
    T7
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71577-plasmid-map-sequence-id-130936

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  30. pJK512

    Plasmid
    #71575
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Arg228>Glu mutant (AarCH6-R228E)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes arginine-228 to glutamate
    Promoter
    T7
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71575-plasmid-map-sequence-id-130934

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  31. pDD2000

    Plasmid
    #101197
    Purpose
    Name: pBluescript-LANApi,K14. Bidirectional reporter driving LANApi side [green luciferase isoform from pCBG68] and K14 side [red luciferase isoform from pCBR] in pBluescript backbone.
    Depositor
    Dirk Dittmer
    Article
    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1
    Insert
    LANApi side [green luciferase isoform of pCBG68], K14 side [red luciferase isoform of pCBR]
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    101197-plasmid-map-sequence-id-194526

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  36. pSECC

    Plasmid
    #60820
    Purpose
    3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre
    Depositor
    Tyler Jacks
    Article
    Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature
    Inserts
    Cas9
    Cre
    Use
    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    BsmBI site eliminated by C->A; E308G
    Promoter
    EFS and EFS (after Cas9-2A)
    Available since
    Nov. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    60820-plasmid-map-sequence-id-371636

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  38. p420-3

    Plasmid
    #71257
    Purpose
    luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter
    Depositor
    Peter Cockerill
    Article
    Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.
    Insert
    3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    GM-CSF minimal
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    71257-plasmid-map-sequence-id-131889

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Showing: 1201 - 1240 of 39536 results