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Plasmid#181943PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertHaloTag
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-10Myc
Plasmid#181939PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert10xMyc
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-g418-spGFP(11)
Plasmid#190257PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertspGFP(11)
UseFor endogenous gene taggingTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-mCherry
Plasmid#179789PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmCherry
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-mScarlet
Plasmid#179818PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmScarlet
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-g418-mNG
Plasmid#179821PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNG
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN-PURO-PTP
Plasmid#51711Purposecontains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.DepositorTypeEmpty backboneUseT. brucei genome integration vectorsTagsPTP (ProtC-TEV-ProtA)ExpressionMutationPromoterAvailable sinceMarch 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-mScarletI
Plasmid#201442PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmScarlet-I
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CoA-Snifit(V97T)
Plasmid#197947PurposeExpression of CoA-Snifit in mammalian cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionMammalianMutationPanK=PanK-V97T, D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RPL13A-FKBPx2
Plasmid#104473PurposePlasmid for pop-in/pop-out replacement of RPL13A with an FKBPx2-tagged version.DepositorInsertRPL13Ap (RPL13A Budding Yeast)
UseTagsFKBPx2-HAExpressionYeastMutationincludes the last 354 bp of RPL13A onlyPromoterAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-g418-3mNG
Plasmid#179832PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert3x mNG
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-hygro-mScarletI
Plasmid#179820PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmScarlet-I
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-mCardinal
Plasmid#179796PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmCardinal
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-hygro-BFP
Plasmid#190260PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertBFP
UseFor endogenous gene taggingTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-g418-mScarlet
Plasmid#179823PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmScarlet
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-nanoluc
Plasmid#179797PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertnanoluc
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptKS(-)-FRBx2
Plasmid#104476PurposePlasmid carrying FRBx2 with C-terminal HA tag.DepositorInsertFRBx2 (MTOR Human)
UsePhagemidTagsHA tagExpressionMutationincludes only the FRB domainPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-g418-eYFP
Plasmid#190220PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInserteYFP
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-hygro-mCherry
Plasmid#190262PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmCherry
UseFor endogenous gene taggingTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-BFP
Plasmid#190258PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertBFP
UseFor endogenous gene taggingTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6.1-phleo-3Myc:mNG:3Myc
Plasmid#181937PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNG
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-10Ty
Plasmid#179807PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert10xTy
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-hygro-10Ty
Plasmid#179808PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert10xTy
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-sfCherry2(1-10)
Plasmid#190206PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertsfCherry2(1-10)
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-mNG:HaloTag
Plasmid#179785PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNG::HaloTag
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNCMV 16E6no* I128T
Plasmid#47706PurposeDefective for E6AP bindingDepositorInsertHPV 16 E6no* (E6 HPV)
UseTagsFLAG and HAExpressionMammalianMutationdel first 7 aa, I128T, V42L*PromoterCMVAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSB4C5_pBen-BenR
Plasmid#128127PurposeExpressing BenR transcription factor gene under control of benzoate responsive promoter (pBen) in a feedback loop.DepositorInsertBenR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBenAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNCMV 16E6no* Y54D
Plasmid#47705PurposeDefective for p53 bindingDepositorInsertHPV 16 E6no* (E6 HPV)
UseTagsFLAG and HAExpressionMammalianMutationdel first 7 aa,Y54D, V42L*PromoterCMVAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLentiN 18E6no*
Plasmid#37446PurposeLentiviral expression of HPV 18E6no* in mammalian cellsDepositorInsertHPV 18E6no* (E6 HPV)
UseLentiviralTagsFlag and HAExpressionMammalianMutationV44APromoterCMVAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
NmCherry-SpVasa
Plasmid#66786Purposeexpression of mCherry in Sea Urchin germ cellsDepositorInsertVasa
UseSea urchin, zebrafish, xenopusTagsmCherryExpressionMammalianMutationPromoterSP6Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RPL13A-FKBPx4
Plasmid#104474PurposePlasmid for pop-in/pop-out replacement of RPL13A with an FKBPx4-tagged version.DepositorInsertRPL13Ap (RPL13A Budding Yeast)
UseTagsFKBPx4-HAExpressionYeastMutationincludes the last 354 bp of RPL13A onlyPromoterAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
PA1024_pET21a(+)C-tag
Plasmid#14579DepositorInsert2-nitropropane dioxygenase (PA1024 Pseudomonas aeruginosa)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
2M-T-Mce3R_K262A
Plasmid#225728PurposeTo express Mce3R mutant K262A in E. coliDepositorInsertmce3R
UseTags6xHis-MBPExpressionBacterialMutationK262APromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MelG
Plasmid#225729PurposeTo express MelG (Rv1937) in E. coliDepositorInsertmelG
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-VapBC35
Plasmid#225733PurposeTo express VapBC35(Rv1962) Toxin antitoxin in E. coliDepositorInsertvapBC35
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-M2
Plasmid#225735PurposeTo express M2 (219-399) in E. coliDepositorInsertmce3R
UseTags6xHisExpressionBacterialMutationaa 219-399PromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
2M-T_Mce3R
Plasmid#225726PurposeTo express Mce3R in E. coliDepositorInsertmce3R
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
2M-T-Mce3R_R53A
Plasmid#225727PurposeTo express Mce3R mutant R53A in E. coliDepositorInsertmce3R
UseTags6xHis-MBPExpressionBacterialMutationR53APromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-TrxC
Plasmid#225742PurposeTo express TrxC(Rv3914) in E. coliDepositorInsertTrxC
UseTags6xHisExpressionBacterialMutationTrxCPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-TrxB2
Plasmid#225741PurposeTo express TrxB2(Rv3913) in E. coliDepositorInsertTrxB2
UseTags6xHisExpressionBacterialMutationTrxB2PromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MelI
Plasmid#225730PurposeTo express MelI (Rv1939) in E. coliDepositorInsertmelI
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MelK
Plasmid#225732PurposeTo express MelK (Rv1941) in E. coliDepositorInsertmelK
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-M1
Plasmid#225734PurposeTo express M1 (13-193) in E. coliDepositorInsertmce3R
UseTags6xHisExpressionBacterialMutationaa 13-193PromoterAvailable sinceOct. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MelJ
Plasmid#225731PurposeTo express MelJ (Rv1940) in E. coliDepositorInsertmelJ
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAMC070n1a12
Plasmid#209746PurposeColA BBa_B0014 tetAp SD8 ScTrpRS rrnB1 proB SD8 Lum1PylRS BBaB10014RNAItrpA+ J23100 SD8 MmPylRS T3Te-T7Te DHFRDepositorInserttRNA operon - ScTrpRS - Lum1PylRS - MmPylRS
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTvGPI(EP)-blast-mNG
Plasmid#221051PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNeonGreen
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTvGPI(VSG)-blast-mNG
Plasmid#221050PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNeonGreen
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-mStayGold[E138D]
Plasmid#221049PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmStayGold[E138D]
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-hygro-His:mNG:His
Plasmid#201096PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNeonGreen
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-moxNG
Plasmid#190222PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmoxNeonGreen
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceSept. 8, 2023AvailabilityAcademic Institutions and Nonprofits only