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pINDUCER21 (ORF-EG)

Plasmid

#46948

Purpose

Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

Depositor

Stephen Elledge, Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

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pInducer10-mir-RUP-PheS

Plasmid

#44011

Purpose

Lentiviral shRNA vector (pGIPz based) for inducible knockdown

Depositor

Stephen Elledge

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Tags

RFP

Expression

Mammalian

Promoter

TRE2

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pINDUCER11 (miR-RUG)

Plasmid

#44363

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pUC57-mitoLbNOX

Plasmid

#74448

Purpose

This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

Depositor

Vamsi Mootha

Article

Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

Insert

mitoLbNOX

Tags

Flag tag with linker and mitochondrial targeting …

Mutation

Human codon optimized

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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SFG.CNb30_opt.IRES.eGFP

Plasmid

#22493

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

Codon optimised Calcineurin B mutant 30 (PPP3R1 Human)

Use

Retroviral

Mutation

L124T; K125-LA-Ins

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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PB_tre_dCas9_KRAB

Plasmid

#126030

Purpose

PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

dCas9 fusion with KRAB
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

EF1 alpha and TRE

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-mitoLbNOX-Flag

Plasmid

#234985

Purpose

Expresses mitochondrial LbNOX in human cell lines

Depositor

Denis Titov

Article

Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

Insert

mito-LbNOX

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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PB_tre_dCas9_VP160

Plasmid

#126031

Purpose

PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

dCas9 fusion with VP160
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

EF1 alpha and TRE

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gα15-244-NLuc

Plasmid

#207154

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNA15 (GNA15 Human)

Expression

Mammalian

Mutation

NLuc inserted at position E(244)N

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gαi1-91-NLuc

Plasmid

#207148

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAI1 (GNAI1 Human)

Expression

Mammalian

Mutation

NLuc inserted at position L(91)K

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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SFG.wtCNb_opt.IRES.eGFP

Plasmid

#22492

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

Codon optimised wild type Calcineurin B (PPP3R1 Human)

Use

Retroviral

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pCfB2904(XI-3 MarkerFree)

Plasmid

#73276

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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p5e irg1

Plasmid

#188698

Purpose

Gateway promoter element for the zebrafish irg1 gene

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

irg1 (acod1 Zebrafish)

Use

Synthetic Biology

Expression

Mammalian

Promoter

irg1

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gγ2-LSS-mKATE2(N)

Plasmid

#207160

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNA

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNG2 (GNG2 Human)

Expression

Mammalian

Mutation

N-terminally tagged with LSS-mKATE2

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pTETRIS-cargo

Plasmid

#126033

Purpose

Luciferase reporter construct for the study of cis-repressive activity in long noncoding RNAs. Also confers puromycin resistance.

Depositor

Mauro Calabrese

Article

Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

Type

Empty backbone

Use

Luciferase; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-eGFP-NMHC-IIAΔtailpiece

Plasmid

#35689

Depositor

Tom Egelhoff

Article

Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

Insert

MHC IIA (MYH9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Added stop codon after codon 1928, eliminating th…

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pGEX6P1-UBE2S-UBD

Plasmid

#66713

Purpose

Expresses the UBE2S-UBD fusion protein in E. coli

Depositor

David Komander

Article

Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

Inserts

e2-epf (UBE2S )
isot (USP5 )

Tags

GST-3C

Expression

Bacterial

Promoter

T7

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
CAGGS-EGFP-NLS-3XFLAG-V5

Plasmid

#196090

Purpose

Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

GFP

Tags

C-terminal 3xFLAG and C-terminal V5

Expression

Bacterial and Mammalian

Promoter

CAGGs

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCfB2909(XII-5 MarkerFree)

Plasmid

#73281

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3052(gRNA X-4, XI-3, XII-5)

Plasmid

#73294

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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