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pX330-sgRNA_Dgcr8_1

Plasmid

#73525

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pCY_gRNA_hPAX7-Pro_C6

Plasmid

#160457

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Insert

gRNA_hPAX7-Promoter_C6

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCY_gRNA_hPAX7-Pro_C5

Plasmid

#160456

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Insert

gRNA_hPAX7-Promoter_C5

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCY_gRNA_hPAX7-Pro_C4

Plasmid

#160455

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Insert

gRNA_hPAX7-Promoter_C4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCY_gRNA_hPAX7-Pro_C3

Plasmid

#160454

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Insert

gRNA_hPAX7-Promoter_C3

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pgRNA-backbone

Plasmid

#172517

Purpose

Yeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertion

Depositor

Leonid Kruglyak

Article

Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

Insert

gRNA cassette

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

Plasmid

#136652

Purpose

Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9
sgRNA for St1Cas9
APOBEC-1 (APOBEC1 Human)
UGI
HA tag

Use

CRISPR and Synthetic Biology

Tags

2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

Expression

Mammalian

Mutation

Promoter

CAG and U6

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Ago2_1

Plasmid

#73529

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Ago2_2

Plasmid

#73530

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pgRNAtet-phaZ

Plasmid

#169874

Purpose

Single guide RNA plasmid for Cas9 targeting phaZ in P. putida

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

sgRNA towards phaZ in Pseudomonas putida

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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Construct 9 - U6p_30nt_stgRNA

Plasmid

#81248

Purpose

Expresses 30nt stgRNA

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAtU6-sgRNA

Plasmid

#119775

Purpose

Expression of a target sgRNA in potato

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

AtU6-SmR-sgRNA

Use

Tags

Expression

Plant

Mutation

Promoter

AtU6

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNA500

Plasmid

#149640

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30, Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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T-STOP sgRNA-1

Plasmid

#83346

Purpose

The sgRNA at the stop codon of human Brachyury(T) locus

Depositor

James Thomson

Article

Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

Insert

T-STOP sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AksgRNA

Plasmid

#121955

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b single chimeric gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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py100-sgRNA_hVEGFA

Plasmid

#198282

Purpose

Expression of SpCas9 guide RNA specific for human VEGFA locus

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

py100-sgRNA_hVEGFA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-TPI1_sgRNA1

Plasmid

#201626

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

TPI1 (TPI1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-TPI1_sgRNA2

Plasmid

#201627

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

TPI1 (TPI1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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Construct 11 - U6p_70nt_stgRNA

Plasmid

#81250

Purpose

Expresses 70nt stgRNA

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAdSh.U6.gRNAS1

Plasmid

#58252

Purpose

U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

Depositor

Manuel Goncalves

Article

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

U6.gRNAS1 cassette

Use

Adenoviral and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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