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Flag-HA-COPS5

Plasmid

#22541

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

COPS5 (COPS5 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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EGFPC2-Gephyrin P1

Plasmid

#68815

Purpose

expression of fluorescent tagged Geph in mammalian cells

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Tags

EGFP

Expression

Mammalian

Mutation

silent mutations on internal EcoRI site. Gephyri…

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCT310

Plasmid

#111140

Purpose

Bacterial expression of 6His-dCas9-3XFlag

Depositor

Robert Tjian

Article

Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

Insert

dCas9

Tags

3X Flag and 6X His

Expression

Bacterial

Mutation

D10A - H840A

Promoter

T7

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-LYN

Plasmid

#23905

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LYN (LYN Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCS2 HRS-RFP

Plasmid

#29685

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

hepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)

Tags

RFP

Expression

Mammalian

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 SHP2 WT

Plasmid

#8322

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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pDONR223-ABL1

Plasmid

#23939

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ABL1 (ABL1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA4 huZAP(S)

Plasmid

#45906

Depositor

Harmit Malik

Article

Kerns et al PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1

Insert

ZAP(S) isoform (ZC3HAV1 Human)

Tags

HA

Expression

Mammalian

Mutation

M202T, L388F

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pZW1-snoVector

Plasmid

#73174

Purpose

Nuclear expression of RNAs

Depositor

Ling-Ling Chen

Article

Yin et al Nucleic Acids Res. 2015 Jan;43(1):e5. do

Inserts

SNORD116-13 (SNORD116-13 Human)
SNORD116-14 (SNORD116-14 Human)

Expression

Mammalian

Promoter

CMV promoter

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV JMJD3

Plasmid

#21212

Depositor

Paul Khavari

Article

Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

Insert

JMJD3 (KDM6B Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Available Since

May 22, 2009

Availability

Academic Institutions and Nonprofits only

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pmtb-t7-alpha-bungarotoxin

Plasmid

#69542

Purpose

For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

Depositor

Sean Megason

Article

Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

Insert

alpha-bungarotoxin

Use

Tol2, beta-actin promoter, sp6,t7 for zebrafish

Promoter

beta-actin, sp6, t7

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR2

Plasmid

#64932

Purpose

Enhanced FRET-based biosensor for monitoring Akt activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

AktAR2

Tags

Cerulean3 and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pDCC6

Plasmid

#59985

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

Depositor

Peter Duchek

Article

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Inserts

Cas9
U6-2>sgRNA

Use

CRISPR

Tags

3xFLAG

Promoter

U6-96Ab and hsp70Bb

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp8 C360A

Plasmid

#11818

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 8 catalytic mutant (CASP8 Human)

Tags

HA

Expression

Mammalian

Mutation

C360A

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

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pLH-spsgRNA2

Plasmid

#64114

Purpose

Vector for expression of Sp sgRNA2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp sgRNA2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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G1A

Plasmid

#24690

Depositor

Jeanne Lawrence

Article

Clemson et al J Cell Biol. 1996 Feb . 132(3):259-75.

Insert

human XIST (XIST Human)

Use

Plasmid cloning vector

Mutation

Genomic insert. Includes introns 5-8.

Available Since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pDS221

Plasmid

#34981

Purpose

Expresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

ePDZb1–mCherry

Expression

Yeast

Promoter

TEF

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pAD-LyseR

Plasmid

#99244

Purpose

For autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

Depositor

Jeff Hasty

Article

Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

Insert

phage lambda gene R (R phage lambda)

Expression

Bacterial

Promoter

beta-lactamase promoter

Available Since

Oct. 14, 2017

Availability

Academic Institutions and Nonprofits only

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HER2 CA (V659E)

Plasmid

#16259

Depositor

Mien-Chie Hung

Article

Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

Insert

HER2 (ERBB2 Human)

Expression

Mammalian

Mutation

Constitutively active: V659E

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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HA-Beclin 1

Plasmid

#86748

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

human Beclin 1 (BECN1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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