59,541 results

1. pMIR-21 Reporter

   Plasmid

   #71870

   Purpose

   Mammalian expression vector for the analysis of miR-21 activity

   Depositor

   Mien-Chie Hung

   Article

   Shen et al Nature. 2013 May 16;497(7449):383-7. doi

   Insert

   Reverse complementary sequence of miR-21
   

   Tags

   firefly luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2324

   Plasmid

   #144010

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TAF1 (TAF1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1

   Plasmid

   #107407

   Purpose

   Lentiviral expression of gRNA resistant PBRM1

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   sg resistant PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Mutation

   sgRNA resistant

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CLYBL hOPM

   Plasmid

   #112499

   Purpose

   Homologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in CLYBL locus

   Depositor

   Bruce Conklin

   Insert

   OTX2, PAX6, MITF (PAX6, MITF, OTX2 Human)
   

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Hpep1-betaArrestin2-T2A-mTagBFP2

   Plasmid

   #194680

   Purpose

   CMV driven co-expression of peptide complementing GPCR recorder (beta-arrestin 2 fusion) and mTagBFP2

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Hpep1-betaArrestin2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV_CMV::V5-EGFR

   Plasmid

   #199717

   Purpose

   Expresses V5-tagged EGFR in mammalian cells.

   Depositor

   Alice Ting

   Article

   Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

   Insert

   V5-EGFR
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK322 (TOP2A-mAC (Hygro))

   Plasmid

   #140653

   Purpose

   TOP2A tagging with mAID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-opto-hMLKL

   Plasmid

   #208789

   Purpose

   Inducibly expresses human MLKL, fused to light-activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   MLKL (MLKL Human)
   

   Use

   Lentiviral

   Tags

   Cry2 mCherry

   Expression

   Mammalian

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tet-O-FUW-KDM2B-P2A-mCherry

   Plasmid

   #203852

   Purpose

   Tet-inducible lentiviral plasmid expressing KDM2B-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   KDM2B (KDM2B Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAlbumin

    Plasmid

    #22037

    Depositor

    Didier Trono

    Insert

    partial sequence of human albumine
    

    Use

    Lentiviral

    Available Since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. mTagBFP2-TOMM20-N-10

    Plasmid

    #55328

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pE-SUMO CRYAA

    Plasmid

    #80756

    Purpose

    Expression of human alpha A-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYAA (CRYAA Human)
    

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-FLAG-USP13

    Plasmid

    #61741

    Purpose

    express N-terminally FLAG tagged human USP13

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    USP13 (USP13 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-YTHDC1-FL-mCherry-Cry2

    Plasmid

    #177124

    Purpose

    Express Full-length YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    YTHDC1 (YTHDC1 Human)
    

    Tags

    mCherry-CRY2

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFRT-TODestFLAGHAhFXR1

    Plasmid

    #48694

    Purpose

    Expresses hFXR1

    Depositor

    Thomas Tuschl

    Article

    Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

    Insert

    FlagHA FXR1 (isoform b) (FXR1 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF0584

    Plasmid

    #142651

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SRF (SRF Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF2861

    Plasmid

    #143644

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SREBF2 (SREBF2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF0169

    Plasmid

    #141540

    Purpose

    Lentiviral vector for overexpressing the SIX3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SIX3 (SIX3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. STARD5A

    Plasmid

    #42392

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    STARD5A (STARD5 Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pTCF5 HLA-A*01:01

    Plasmid

    #180448

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*01:01
    

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Available Since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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