164,802 results

1. pN1-CMV-H-sDarken

   Plasmid

   #184800

   Purpose

   High affinity version of the Serotonin Sensor sDarken under the control of a CMV Promoter.

   Depositor

   Olivia Masseck

   Article

   Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

   Insert

   H-sDarken
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAT001

   Plasmid

   #171061

   Purpose

   Plasmid for expressing HIV-1 envelope glycoprotein

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Insert

   HIV-1 Env
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCCL/IFNB1-d2eGFP-3’UTR

   Plasmid

   #180232

   Purpose

   Reporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Front Cell Infect Microbiol. 2022 Jan 18

   Insert

   d2eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   1 kb upstream of IFNB1 CDS

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBR322-Pdp1_NTD-Cas9

   Plasmid

   #198275

   Purpose

   For loading SpCas9 into PVCs; also contains PVC regulatory genes

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   Pdp1_NTD-Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST17-UnaG

   Plasmid

   #203485

   Purpose

   For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

   Insert

   UnaG
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG RpH-LAMP1-3xFLAG

   Plasmid

   #163018

   Purpose

   expresses ratiometric sensor of lysosomal lumenal pH

   Depositor

   Massimiliano Stagi

   Article

   Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

   Insert

   RpH-LAMP1-3xFLAG (Lamp1 Mouse)
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCF806-shRen.713

   Plasmid

   #186712

   Purpose

   Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

   Depositor

   Christof Fellmann

   Article

   Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

   Insert

   shRen.713
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEMG-RIS

   Plasmid

   #225132

   Purpose

   For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Km.

   Depositor

   Lars Blank

   Article

   Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pGWB402

   Plasmid

   #74796

   Purpose

   Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Hygro(+)-mscGAS

    Plasmid

    #102607

    Purpose

    Mammalian expression of mouse cGAS

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    cGAS (Cgas Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a(+)_6xHIS-hHLA-A2 (MHC Class I heavy)-C-Tag

    Plasmid

    #187356

    Purpose

    Bacterial expression and refolding of gp100 pMHC

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    hHLA-A2 (MHC Class I heavy) with N-terminal 6xHIS tag, and C-terminal C-Tag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a(+)_beta2M (MHC Class I light)

    Plasmid

    #187357

    Purpose

    Bacterial expression and refolding of gp100 pMHC

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    beta2M (MHC Class I light)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. HP4_M13

    Plasmid

    #120340

    Purpose

    Helper plasmid for production of phagemid ssDNA, based on m13mp18

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

    Insert

    Coding part of the M13 phage genome
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    native phage promoters

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT3TS-spCas9 ABE-Umax-ex1

    Plasmid

    #222141

    Purpose

    Adenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDonor_Tn[himar1C9]

    Plasmid

    #213906

    Purpose

    Mariner transposon with ATc-inducible himar1C9 transposase

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with cam resistance and ATc-inducible himar1C9 transposase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AiP20027 - pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA

    Plasmid

    #220735

    Purpose

    AiE2182m_3xC2 is an optimized enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

    Depositor

    Bosiljka Tasic, Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre(R297T)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Beta1-GFP in pHcgreen

    Plasmid

    #69804

    Purpose

    Expresses Beta1-GFP integrin for normal cell transfection

    Depositor

    Martin Humphries

    Article

    Parsons et al J Cell Sci. 2008 Feb 1;121(Pt 3):265-71.

    Insert

    Beta1A integrin (ITGB1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDESTmycDICER

    Plasmid

    #19873

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    dicer 1, ribonuclease type III (DICER1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. VP12

    Plasmid

    #72247

    Purpose

    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV5 TBRI-HA with various GS domain mutations

    Plasmid

    #19162

    Depositor

    Joan Massague

    Insert

    transforming growth factor, beta receptor 1 (TGFBR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T204D (GS domain mutation)

    Promoter

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCSIIpuro2-H2B-iRFP

    Plasmid

    #197352

    Purpose

    Encoding nuclear-targeted iRFP713.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    iRFP with nucleus-targeting sequence
    

    Use

    Lentiviral

    Tags

    H2B (human histone 2B)

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. gCH128 (non-targeting crRNA)

    Plasmid

    #217339

    Purpose

    Non-targeting crRNA

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    crNT-3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MigR1-AE9a

    Plasmid

    #12433

    Depositor

    Dong-Er Zhang

    Article

    Yan et al Nat Med. 2006 Aug . 12(8):945-9.

    Insert

    AML1-ETO9a fusion (RUNX1, RUNX1T1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AML-ETO protein with ETO exon 9a.

    Promoter

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pV1524

    Plasmid

    #111431

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAGGs-BFP

    Plasmid

    #127348

    Purpose

    Expresses BFP constitutively via the CAGGS promoter in mammalian cells

    Depositor

    Phil Sharp

    Insert

    Blue Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS Promoter

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CMVTO-Furin

    Plasmid

    #179920

    Purpose

    Furin protein

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    Furin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. JpExpress404 PTEN-LONG

    Plasmid

    #49417

    Purpose

    Bacterial Expression Vector

    Depositor

    Ramon Parsons

    Article

    Hopkins et al Science. 2013 Jul 26;341(6144):399-402.

    Insert

    PTEN-Long (PTEN )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBR_attB(bxb)_lox

    Plasmid

    #183762

    Purpose

    Bxb1-specific donor plasmid for the cloning of payloads <20 kb

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mCherry-APP-mGFP

    Plasmid

    #196704

    Purpose

    Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mCherry and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTn7CdrA-gfpC

    Plasmid

    #111616

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge