159,589 results

1. pYFP-ErbB2

   Plasmid

   #66948

   Purpose

   Mammalian expression of rat ErbB2 tagged with YFP

   Depositor

   Mads Lerdrup, Bo van_Deurs

   Article

   Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

   Insert

   ErbB2 (Erbb2 Rat)
   

   Use

   Tags

   6xHis, V5 Tag, and YFP

   Expression

   Mammalian

   Mutation

   (See depositor comment below on mutations A24T an…

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

2. prDNA spacer-LacOx50-20xMS2-Hyg

   Plasmid

   #210219

   Purpose

   Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   50xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. mTagRFP-T-Mito-7

   Plasmid

   #58023

   Purpose

   Localization: Mitochondria, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #191797

   Purpose

   vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized dCas13d
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   R239A, H244A, R858A, H863A

   Promoter

   CBh, SV40, hU6

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-myc-CAR-2A-Thy1.1

   Plasmid

   #127890

   Purpose

   Expresses CART receptor along with tags.

   Depositor

   Anjana Rao

   Article

   Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

   Inserts

   myc,
   CAR
   2A
   Thy1.1
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAVS1-Pur-CAG-mCherry

   Plasmid

   #80946

   Purpose

   human AAVS1 site targeting donor plasmid for knocking-in mCherry expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2911

   Plasmid

   #143666

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFIC (NFIC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDRF1-GW ymNeongreen-T2A-mCherry

   Plasmid

   #125700

   Purpose

   Produces equimolar expression of yeast codon optimized mNeongreen and mCherry

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeongreen-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pwtCas9-bacteria

   Plasmid

   #44250

   Purpose

   aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   wild-type Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available since

   April 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENN.AAV.CB7.CI.mCerulean.WPRE.RBG

    Plasmid

    #105557

    Purpose

    AAV expression of mCerulean from CB7 promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1 and AAV9

    Insert

    mCerulean
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CB7

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. RFP-zyxin

    Plasmid

    #26720

    Depositor

    Anna Huttenlocher

    Article

    Bhatt et al J Cell Sci. 2002 Sep 1. 115(Pt 17):3415-

    Insert

    Zyxin (ZYX Human, unknown)
    

    Use

    Tags

    RFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXspuro-mCherry-NBR1

    Plasmid

    #74242

    Purpose

    Retroviral expression of mCherry-NBR1

    Depositor

    Jayanta Debnath

    Article

    Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

    Insert

    neighbor of BRCA1 gene 1 (NBR1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pMJ114

    Plasmid

    #85995

    Purpose

    One-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant region

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    sgGFP-NT2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    modified bovine U6-2

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Bxb1-GA Donor_hEF1a

    Plasmid

    #229779

    Purpose

    Bxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment marker

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSAM2_mCherry_Gata6

    Plasmid

    #72694

    Purpose

    Doxycyline regulated expression of Gata6 in mammalian cells

    Depositor

    Timothy Kamp

    Article

    Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

    Insert

    Gata 6 (Gata6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-Doxycycline

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCasso

    Plasmid

    #207530

    Purpose

    Conditionally-replicating in Pseudomonas plasmid for cytidine base editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sg

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    cytidine base editing; oriV(pRO1600/ColE1), xylS, Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sgRNA
    

    Use

    CRISPR; Pseudomonas vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-CHMP4B

    Plasmid

    #139013

    Purpose

    Expresses HA-tagged human CHMP4B in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Insert

    human CHMP4B
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDG459

    Plasmid

    #100901

    Purpose

    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-TVAmCherry-2A-oG

    Plasmid

    #104330

    Purpose

    AAV constitutively expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG)

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    TVA-mCherry fusion protein
    Optimised Rabies Glycoprotein (oG)
    

    Use

    AAV; Adeno associated viral vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. psd44-GqQL

    Plasmid

    #46826

    Purpose

    2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells

    Depositor

    Agnese Mariotti

    Insert

    g protein alpha q (GNAQ Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Q209L (The mutation reduces GTPase activity resul…

    Promoter

    PGK promoter

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. CaVα2δ1

    Plasmid

    #26575

    Depositor

    Diane Lipscombe

    Article

    Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

    Insert

    Cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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22. CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)

    Plasmid

    #225752

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mouse E-cadherin GFP (423)

    Plasmid

    #67937

    Purpose

    Mammalian expression of E-cadherin-GFP

    Depositor

    Alpha Yap

    Article

    Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

    Insert

    E-cadherin (Cdh1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLminP_Luc2P_RE59

    Plasmid

    #90404

    Purpose

    STAT3 - Sis-inducible element (SIE) gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    IRF1

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. h-Menkes

    Plasmid

    #196953

    Purpose

    Plasmid expressing an antigen targeting the Menke's disease copper ATPase

    Depositor

    James Trimmer

    Insert

    Copper ATPase 1 (ATP7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCI pHluorin Syt7

    Plasmid

    #184508

    Purpose

    Mammalian expression of Synaptotagmin 7 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 7 (Syt7 Mouse)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLL7.0: Venus-iLID-Mito (From ActA)

    Plasmid

    #60413

    Purpose

    Mammalian Expression of Venus-iLID-Mito

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-iLID-Mito
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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28. SIRT6 Flag

    Plasmid

    #13817

    Purpose

    Mammalian expression of human SIRT6 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT6 (SIRT6 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEA186

    Plasmid

    #187874

    Purpose

    Empty pVS1-based INTEGRATE vector with mCherry cargo

    Depositor

    Kan Wang

    Article

    Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAIP hIL2 co

    Plasmid

    #90513

    Purpose

    Lentiviral vector for expression of human codon optimized IL2 with puromycin resistance.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    IL2 (IL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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