We narrowed to 8,358 results for: BLI

1. pAAV Syn 2xLyn-ERex-mKate2-bPAC(F198Y)

   Plasmid

   #219654

   Purpose

   Neuronal expression of PACmn with mKate2, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity.

   Depositor

   Thomas Oertner

   Insert

   2xLyn-ERex-mKate2-bPAC(F198Y)
   

   Use

   AAV

   Tags

   ER exit ( FCYENE) and Lyn11 (GCIKSKGKDS)

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

   Plasmid

   #105715

   Purpose

   In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.

   Depositor

   Rylan Larsen

   Has Service

   AAV5

   Insert

   synaptophysin-GCaMP6s
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a, Human elongation factor-1 alpha

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBabe-Kras G12V-T35S

   Plasmid

   #58900

   Purpose

   Expresses human Kras with G12V and T35S mutations

   Depositor

   Channing Der

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G12V and T35S mutations (effector binding mutant)

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

4. Hs.KRAS4b G12R

   Plasmid

   #83143

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12R mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   G12R

   Promoter

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MECR

   Plasmid

   #38850

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MECR (Mecr Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. p413TEF1-ATR1(pYL573)

   Plasmid

   #178288

   Purpose

   Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   ATR1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG416-GAL-FUS

   Plasmid

   #181711

   Purpose

   Galactose inducible expression of FUS

   Depositor

   Alejandro Chavez

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. huntingtin_V2095-V3138_NbioCflag

   Plasmid

   #194765

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

   Depositor

   Cheryl Arrowsmith

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   Avi-tag (biotin acceptor peptide) and Flag-tag

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV and P10

   Available since

   July 26, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. CasRX_Pac

   Plasmid

   #194803

   Purpose

   CasRX vector for gene knockdown in Giardia. Insert gRNA using Bbs1 restriction site. Puromycin resistance cassette.

   Depositor

   Alexander Paredez

   Article

   Shih et al Proc Natl Acad Sci U S A. 2022 May 31;11

   Insert

   CasRX
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DOT1L

    Plasmid

    #36196

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DOT1L (GeneScript Synthesized) (DOT1L Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acid residues 1-420

    Promoter

    Available since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

11. Hs.KRAS4b Q61L

    Plasmid

    #83134

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61L mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Q61L

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p-dCas9-p300-Hygro

    Plasmid

    #104411

    Purpose

    transient expression of dCas9-p300 fusion protein

    Depositor

    Stephan Beck

    Insert

    p300 (EP300 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-DIO-EF1a-TVA-P2A-EYFP

    Plasmid

    #177017

    Purpose

    For expression of the avian TVA receptor in a cre-dependent manner

    Depositor

    Peter Jonas

    Insert

    TVA-P2A-EYFP (CD320 Coturnix japonica)
    

    Use

    AAV and Cre/Lox

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQTEV-SNX3

    Plasmid

    #31573

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    sorting nexin 3 (SNX3 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET3C-ARL16(CO)-His6

    Plasmid

    #216485

    Purpose

    Used for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.

    Depositor

    Richard Kahn

    Insert

    ADP-ribosylation factor-like 16 (ARL16 Human)
    

    Use

    Tags

    includes GSGSGSHHHHHH (His6) tag at C-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Hs.KRAS4b G13C

    Plasmid

    #83145

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13C mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    G13C

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. C1QBP

    Plasmid

    #32863

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    C1QBP (C1QBP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    amino acids E89-Q282 only

    Promoter

    Available since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. BASIC_SEVA_66.11

    Plasmid

    #194213

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pINDUCER20-FLAG-hPAX3 (#735)

    Plasmid

    #78336

    Purpose

    Doxycycline-inducible lentiviral overexpression of human PAX3 ORF with a FLAG tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    PAX3 (PAX3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    TRE2

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

20. ACE2 g2

    Plasmid

    #153012

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

    Depositor

    Jason Sheltzer

    Insert

    ACE2 gRNA (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge