We narrowed to 10,337 results for: plasmids 101

1. pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST

   Plasmid

   #127316

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7

   Plasmid

   #124859

   Purpose

   Mutagenesis of Slc17a7

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a7 (Slc17a7 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST

   Plasmid

   #127318

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pClneoFH-let60 G12V

   Plasmid

   #66860

   Purpose

   Express FLAG-His6-tagged dominant active let60, in which Glycine 12 is mutated to Valine, in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

   Insert

   Let-60 (let-60 Nematode)
   

   Use

   Tags

   FLAG-His6

   Expression

   Mammalian

   Mutation

   Glycine 12 is mutated to valine

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pRS316-VTC4

   Plasmid

   #108691

   Purpose

   VTC4 sequence expressed in yeast plasmid

   Depositor

   Michael Downey

   Article

   Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

   Insert

   Vacuolar Transporter Chaperone 4
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   VTC4 (native promoter)

   Available Since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. psiCheck-ApoE3'UTR

   Plasmid

   #41846

   Depositor

   Sohail Tavazoie

   Article

   Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

   Insert

   ApoE 3'UTR (APOE Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pJ02B2Gm:Rm_AF

   Plasmid

   #66061

   Purpose

   MoClo Device: FACS Standard Color Controls - High GFP (first unit) & RFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E:J23102:B:BCD2:C:E1010m:D:B0015:F]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   FACS Controls
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJ02B2Rm:Gm_AF

   Plasmid

   #66062

   Purpose

   MoClo Device: FACS Standard Color Controls - High RFP (first unit) & GFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E:J23102:B:BCD2:C:E0040m:D:B0015:F]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   FACS Controls
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. t2. pT7-7-SNCA-5Lys

   Plasmid

   #107294

   Purpose

   Plasmid for the expression of the construct SNCA-5Lys. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Lys and 7 Gly

   Depositor

   Dmytro Yushchenko

   Article

   Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

   Insert

   SNCA-5Lys (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. r. pT7-7-SNCA-5Asn

    Plasmid

    #107295

    Purpose

    Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asn and 7 Gly

    Depositor

    Dmytro Yushchenko

    Article

    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

    Insert

    SNCA-5Asn (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. s2. pT7-7-SNCA-5Asp

    Plasmid

    #107293

    Purpose

    Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asp and 7 Gly

    Depositor

    Dmytro Yushchenko

    Article

    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

    Insert

    SNCA-5Asp (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. gh173

    Plasmid

    #106834

    Purpose

    expression of gRNA targeting UGGT2

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    UGGT2 (UGGT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSR04

    Plasmid

    #69151

    Purpose

    read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as gene of interest for integration on cxtTi10816, Mos Chr IV

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    TagBFP
    eGFP
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    eef-1A.1 (eft-3) and rps-27

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZNpF-∆11cag

    Plasmid

    #245370

    Purpose

    Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

    Depositor

    Bruce Hamilton

    Article

    Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

    Insert

    Zfp423-∆11cag (Zfp423 Mouse)
    

    Use

    Luciferase and Synthetic Biology

    Tags

    NanoLuciferase

    Expression

    Mammalian

    Mutation

    11-bp deletion, H96Wfs*4, plus 3 synonymous mutat…

    Promoter

    EF1a

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pZNpF-∆7cg

    Plasmid

    #245371

    Purpose

    Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

    Depositor

    Bruce Hamilton

    Article

    Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

    Insert

    Zfp423-∆7cg (Zfp423 Mouse)
    

    Use

    Luciferase and Synthetic Biology

    Tags

    NanoLuciferase

    Expression

    Mammalian

    Mutation

    7-bp deletion, H96Vfs*29, plus 2 synonymous mutat…

    Promoter

    EF1a

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUBC(k)-AT5G42080-mKOk

    Plasmid

    #224852

    Purpose

    Plasmid for expression of AT5G40280.1 coding sequence tagged with mKOk in plants

    Depositor

    Rebecca Roston

    Article

    LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

    Insert

    AT5G40280.1 (ERA1 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCLJ-Fgf5-t2A-eGFP

    Plasmid

    #227504

    Purpose

    Inserts t2a-eGFP at the 3' end of the Fgf5 gene alsong with a removable selection cassette

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFNC-5

    Plasmid

    #233297

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. TcR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Inserts

    BxbI phage integrase
    sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PEM7 and unknown

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-hU6-cs1-PuroR-T2A-BFP

    Plasmid

    #230934

    Purpose

    gRNA cloning vector with direct capture sequence 1 in stem loop 2

    Depositor

    Charles Gersbach

    Article

    Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFNC-6

    Plasmid

    #227669

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. GmR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Inserts

    BxbI phage integrase
    sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PEM7

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge