4,178 results

1. pMSCV-IRES-YFP II (pMIY II)

   Plasmid

   #52108

   Purpose

   a bicistronic IRES-YFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-EYFP

   Expression

   Mammalian

   Available Since

   March 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-EGFP-AID-BubR1

   Plasmid

   #47330

   Purpose

   for plasmid integration using the Flp-In system. AID at N-terminal

   Depositor

   Don Cleveland

   Article

   Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

   Insert

   BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
   

   Tags

   AID and GFP

   Expression

   Mammalian

   Mutation

   siRNA resistant

   Available Since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. aMHC-mCherry-Rex-Blasticidin

   Plasmid

   #21228

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   alpha myosin heavy chain promoter
   

   Use

   Lentiviral

   Tags

   mCherry

   Available Since

   June 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9(BB)-2A-Blast

   Plasmid

   #118055

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-iCre

   Plasmid

   #89573

   Purpose

   Expresses iCre under pCAG

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   iCre
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET29b-10xHis-Super TEV

   Plasmid

   #193833

   Purpose

   Expresses high-efficiency TEV protease in bacterial cells

   Depositor

   EPFL Protein Production and Structure Core Facility (PTPSP), Florence Pojer

   Article

   Lau et al protocols.io, doi: 10.17504/protocols.io

   Insert

   TEV protease
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC0043-PspCas13b crRNA backbone

   Plasmid

   #103854

   Purpose

   For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6-BbsI-BbsI-PspCas13b DR-polyT
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 28, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCDH-EF1a-eFFly-mCherry

   Plasmid

   #104833

   Purpose

   3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

   Depositor

   Irmela Jeremias

   Article

   Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

   Inserts

   eFFly
   mCherry
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUW-M2rtTA

   Plasmid

   #20342

   Purpose

   Lentiviral plasmid expressing the reverse tetracycline transactivator

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

   Insert

   M2rtTA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPR v2-Blast

    Plasmid

    #83480

    Purpose

    Mammalian expression of Cas9 and sgRNA scaffold

    Depositor

    Mohan Babu

    Insert

    Blasticidin S deaminase
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    Replaced puromycin N-acetyltransferase on the ori…

    Promoter

    EF-1α

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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