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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 10,920 results for: NSI;

Showing: 1241 - 1280 of 10920 results

  4. RS18960_pMQ30_KO_Kan

    Plasmid
    #185393
    Purpose
    Non-replicating vector used to create markerless deletion of RR42_RS18960 in C. basilensis 4G11
    Depositor
    Adam Arkin
    Article
    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1
    Inserts
    RR42_RS18960 upstream flanking homology region
    RR42_RS18960 downstream flanking homology region
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    185393-plasmid-map-sequence-id-371207

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  5. RS17060_pMQ30_KO_Kan

    Plasmid
    #185396
    Purpose
    Non-replicating vector used to create markerless deletion of RR42_RS17060 in C. basilensis 4G11
    Depositor
    Adam Arkin
    Article
    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1
    Inserts
    RR42_RS17060 upstream flanking homology region
    RR42_RS17060 downstream flanking homology region
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    185396-plasmid-map-sequence-id-371203

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  6. RS17055_pMQ30_KO_Kan

    Plasmid
    #185398
    Purpose
    Non-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11
    Depositor
    Adam Arkin
    Article
    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1
    Inserts
    RR42_RS17055 upstream flanking homology region
    RR42_RS17055 downstream flanking homology region
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    185398-plasmid-map-sequence-id-366185

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  10. HpMscS

    Plasmid
    #78310
    Purpose
    H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag
    Depositor
    Douglas Rees
    Article
    Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:
    Insert
    Mechanosensitive channel of small conductance
    Use
    Tags
    6x His Tag and FLAG Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    June 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    78310-plasmid-map-sequence-id-151301

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  12. pUWR-CadTS

    Plasmid
    #58325
    Purpose
    Destination vector for inserting ubi-CadTS to Drosophila melanogaster genome
    Depositor
    Denise Montell
    Article
    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1
    Insert
    E-cadherin tension sensor
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    poly-ubiquitin
    Available since
    Aug. 26, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pUE1-TSP-ORF68 K395A/K396A

    Plasmid
    #162656
    Purpose
    Expresses ORF68 K395A/K396A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1
    Insert
    ORF68
    Use
    Tags
    N-terminal 2xStrep-Tag II and HRV 3C site
    Expression
    Mammalian
    Mutation
    Amino acids K395 and K396 mutated to alanine
    Promoter
    CMV
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    162656-plasmid-map-sequence-id-320674

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  15. As3 (pBW411J117-arsR)

    Plasmid
    #123142
    Purpose
    An arsenic sensor with optimized intracellular receptor density. Also used in the sensor cell array. pSB3K3 carrying J117-30arsR-t-ParsR-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J117-30arsR-t-ParsR-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J117, ParsR
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123142-plasmid-map-sequence-id-241980

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  16. pCfB2188-PrARG8-GFP::URA3

    Plasmid
    #216512
    Purpose
    Integrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeast
    Depositor
    Juha Saarikangas
    Article
    Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.
    Insert
    PrARG8-yeGFP
    Use
    Cre/Lox; Integrative
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ARG8
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    216512-plasmid-map-sequence-id-429384

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  17. pCfB2197-PrGNP1-mK2-NatMX

    Plasmid
    #216513
    Purpose
    Integrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeast
    Depositor
    Juha Saarikangas
    Article
    Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.
    Insert
    PrGnp1-mKate2
    Use
    Cre/Lox; Integrative
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GNP1
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    216513-plasmid-map-sequence-id-429383

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  27. Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent

    Plasmid
    #229800
    Purpose
    Bxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a
    Depositor
    Richard Davis
    Article
    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    mGreenLantern; mRuby2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1a and CAG
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    229800-plasmid-map-sequence-id-461681

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  40. pTcI38-PRM-wasabi

    Plasmid
    #195554
    Purpose
    Temperature-dependent expression of mWasabi reporter with transcription activation by TcI38
    Depositor
    Mikhail Shapiro
    Article
    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2
    Inserts
    mWasabi
    TcI38
    Use
    Tags
    6x His and AANDENYADAS degradation tag
    Expression
    Bacterial
    Mutation
    A67T, M1V, L65S, K68R, F115L, D126G, D188G
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    195554-plasmid-map-sequence-id-426585

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Showing: 1241 - 1280 of 10920 results