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We narrowed to 1,593 results for: PTS

Showing: 1551 - 1593 of 1593 results

  7. pAAV-HDC-DIO-GFP-shRNA.scramble

    Plasmid
    #184331
    Purpose
    Expresses scramble shRNA for control expts. Flexed (DIO) cassette driven by hdc promoter fragment for pan neuronal expression
    Depositor
    William Wisden
    Article
    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.
    Insert
    hdc pan neuronal gene promoter, flex switch, GFP, scramble shRNA
    Use
    AAV, Cre/Lox, Mouse Targeting, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    histidine decarboxylase promoter fragment that g…
    Available since
    June 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    184331-plasmid-map-sequence-id-365459

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  10. GST-CHMP4A (205-222) E209A (pGEX2T(TEV))

    Plasmid
    #80640
    Purpose
    Residues 205-222 of CHMP4A, Mutation modestly disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-206
    Depositor
    Wesley Sundquist
    Article
    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105
    Insert
    CHMP4A (CHMP4A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Changed Glutamate 209 to alanine
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80640-plasmid-map-sequence-id-161707

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  14. GST-CHMP4A (205-222) L217A (pGEX2T(TEV))

    Plasmid
    #80638
    Purpose
    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-204
    Depositor
    Wesley Sundquist
    Article
    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105
    Insert
    CHMP4A (CHMP4A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Residues 205-222; Changed Leucine 217 to Alanine
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80638-plasmid-map-sequence-id-161738

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  15. GST-CHMP4A (205-222) W220A (pGEX2T(TEV))

    Plasmid
    #80637
    Purpose
    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-203
    Depositor
    Wesley Sundquist
    Article
    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105
    Insert
    CHMP4A (CHMP4A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Residues 205-222; Changed Trp 220 to Ala
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80637-plasmid-map-sequence-id-161733

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  16. GST-CHMP4A (205-222) L214A (pGEX2T(TEV))

    Plasmid
    #80639
    Purpose
    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-205
    Depositor
    Wesley Sundquist
    Article
    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105
    Insert
    CHMP4A (CHMP4A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Residues 205-222; Changed Leucine 214 to Alanine
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80639-plasmid-map-sequence-id-161725

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  17. MLRV

    Plasmid
    #118069
    Purpose
    Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters
    Depositor
    Koen Venken
    Article
    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:
    Inserts
    TB:5xNF-κβ:RedF:bGHpA
    TB:4xTGF-β:FLuc:bGHpA
    TB:5xE-box:Renilla:bGHpA
    TB:2xp53:NLuc:bGHpA
    TB:6xAP-1:GrRenilla:bGHpA
    CMV:ELuc:bGHpA
    Use
    Luciferase and Synthetic Biology; Assembly of gb2…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    118069-plasmid-map-sequence-id-240503

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  19. pGEM-2t

    Plasmid
    #159752
    Purpose
    CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    sgRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159752-plasmid-map-sequence-id-313824

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  21. pLX304-YAP1 _60-89

    Plasmid
    #59144
    Purpose
    YAP1 mutants that harbored a deletion of amino acide at 60-89 position, which disrupts binding to TEAD transcription factors
    Depositor
    William Hahn
    Article
    Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.
    Insert
    YAP1 (YAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    aa60-89 deleted
    Promoter
    CMV
    Available since
    Aug. 19, 2014
    Availability
    Academic Institutions and Nonprofits only

  24. AP2u2-CMV-S_protein

    Plasmid
    #226456
    Purpose
    Mammalian expression of S protein of SARS-CoV2 under CMV promoter, tagged with 6His and released extracellular environment after expression for protein purification
    Depositor
    Urartu Seker
    Article
    Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(
    Insert
    Spike Protein
    Use
    Tags
    6xHis Tag, Foldon Domain, and Thrombin Cleavage S…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    226456-plasmid-map-sequence-id-458388

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  26. Ad:2CMV_A2R2-N25K3

    Plasmid
    #122242
    Purpose
    Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone
    Depositor
    Corey Neu
    Article
    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516
    Inserts
    C-terminal end of Nesprin-3 (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    Use
    Adenoviral
    Tags
    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    122242-plasmid-map-sequence-id-238909

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  27. pSH-Csy4-T2A-SpRFN

    Plasmid
    #85754
    Purpose
    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Insert
    Csy4-T2A-FokI-dCas9
    Use
    CRISPR
    Tags
    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85754-plasmid-map-sequence-id-170272

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  29. pSH-Csy4-T2A-SaRFN

    Plasmid
    #85755
    Purpose
    Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Insert
    Csy4-T2A-FokI-SadCas9
    Use
    CRISPR
    Tags
    3xHA tag, Csy4, and FokI fusion via 36 amino acid…
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    CAG
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85755-plasmid-map-sequence-id-170274

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  31. AP2u2-CMV-Ace2-IgG1

    Plasmid
    #226455
    Purpose
    Mammalian expression of human Ace2 protein under CMV promoter fused with IgG1, tagged with 6His, and released extracellular environment after expression for protein purification
    Depositor
    Urartu Seker
    Article
    Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(
    Inserts
    Angiotensin Converting Enzyme 2
    Immunoglobulin Heavy Constant Gamma 1
    Use
    Tags
    6xHis Tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    226455-plasmid-map-sequence-id-458394

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  33. pMX-53BP1(1-1710) 3LA

    Plasmid
    #185698
    Purpose
    Mammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks.
    Depositor
    Daniel Durocher
    Article
    Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.
    Insert
    53BP1(1-1710) 3LA (TP53BP1 Human)
    Use
    Retroviral
    Tags
    Flag tag, HA tag, and tetracysteine tag
    Expression
    Mammalian
    Mutation
    L175A, L517A, and L693A
    Promoter
    SV40
    Available since
    July 7, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185698-plasmid-map-sequence-id-365946

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  34. pMX-53BP1(1-1710) 3LA7A

    Plasmid
    #185707
    Purpose
    Mammalian Expression, Retroviral vector that encodes 7A and 3LA mutant of 53BP1. Prevents shieldin and RIF1 recruitment to sites of double-strand breaks and disrupts 53BP1 activity.
    Depositor
    Daniel Durocher
    Article
    Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.
    Insert
    53BP1(1-1710) 3LA7A (TP53BP1 Human)
    Use
    Retroviral
    Tags
    Flag tag, HA tag, and tetracysteine tag
    Expression
    Mammalian
    Mutation
    T302A, S452A, S523A, S543A, S625A, S784A, and S89…
    Promoter
    SV40
    Available since
    July 1, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185707-plasmid-map-sequence-id-365947

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  37. pDONR223_DNTTIP2_WT_V5

    Plasmid
    #82939
    Purpose
    Gateway Donor vector containing DNTTIP2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.
    Depositor
    Jesse Boehm, David Root, Matthew Meyerson
    Article
    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do
    Insert
    DNTTIP2 (DNTTIP2 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    E309D
    Promoter
    None
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82939-plasmid-map-sequence-id-162410

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  39. pUAP1

    Plasmid
    #63674
    Purpose
    Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.
    Depositor
    Nicola Patron
    Article
    Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np
    Insert
    RFP cloning selection cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    63674-plasmid-map-sequence-id-106088

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Showing: 1551 - 1593 of 1593 results