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pCas9-EcRT-GAL-Kan

Plasmid

#232092

Purpose

Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

Depositor

Jeffrey Lewis

Article

Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

Insert

SpCas9 and Ec86 reverse transcriptase

Use

CRISPR

Tags

Expression

Yeast

Mutation

Ec86 reverse transcriptase is codon optimized for…

Promoter

GAL1-GAL10

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-GFP

Plasmid

#39289

Depositor

John Pringle, Jurg Bahler

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

-1163 to +6 of nmt1 locus

Promoter

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-ymScarletI3-Kan

Plasmid

#224077

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selection

Depositor

Bas Teusink

Insert

ymScarletI3

Use

Tags

ymScarletI3

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK-BB1 [Backbone_RK2-KanR_PartTypeBB]

Plasmid

#229387

Purpose

XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_RK2-KanR_PartTypeBB and an sfGFP dropout

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomCherry-Kan

Plasmid

#44903

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mCherry with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pBx-Spas-sgRNA-Kan

Plasmid

#105233

Purpose

Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescens

Depositor

Kristala Prather

Article

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-3xFLAG-kanMX6

Plasmid

#20754

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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P70a-deGFP-sc101-kan

Plasmid

#184841

Purpose

Expression of deGFP

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

promoter OR2-OR1

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-TID-HA3-KanMX6

Plasmid

#227980

Purpose

To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomTagBFP-Kan

Plasmid

#44898

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mTagBFP with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomTagBFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomRuby-Kan

Plasmid

#44956

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mRuby with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomRuby

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-FLAG-kanMX6

Plasmid

#20751

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pmirGLO:Δluc::gfp ΔAmpR::KanR

Plasmid

#176640

Purpose

MPRAu backbone vector

Depositor

Pardis Sabeti

Article

Griesemer et al Cell. 2021 Sep 30;184(20):5247-5260.e19.

Insert

emGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Human PGK-1

Available since

Feb. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pSUM-Kan-MCS2-gfp

Plasmid

#109290

Purpose

Expresses high levels of gfp in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

Kanamycin

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pDonor14-ShoHELIX-KanR (BO4)

Plasmid

#181795

Purpose

pDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-ymiRFP680-Kan

Plasmid

#224090

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized miRFP680 with Kan selection

Depositor

Bas Teusink

Insert

ymiRFP680

Use

Tags

ymiRFP680

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pfa6a PhoCl-TsCC(B)::KANMX

Plasmid

#185906

Purpose

Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

PhoCl-TsCC(B)

Use

Tags

PhoCl fluorophore with C-terminal TsCC(B)

Expression

Yeast

Mutation

Promoter

SP6

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt-3HA

Plasmid

#39284

Depositor

John Pringle, Jurg Bahler

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Expression

Mutation

-1163 to +6 of nmt1 locus with a 4bp deletion (TA…

Promoter

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P81nmt1-3HA

Plasmid

#39285

Depositor

John Pringle, Jurg Bahler

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Expression

Mutation

-1163 to +6 of nmt1 locus with a 7bp deletion (AT…

Promoter

Available since

Aug. 14, 2012

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P41nmt1-3FLAG

Plasmid

#19337

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

3FLAG

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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