We narrowed to 24,459 results for: Spr

1. LentiCRISPR v2-sgLKB1 clone3

   Plasmid

   #162127

   Purpose

   Lentiviral sgRNA plasmid targeting human LKB1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgLKB1 (STK11 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCRISPRv2-sgMaob-HepmCherry

   Plasmid

   #192829

   Purpose

   Expresses sgRNA targeting Maob and mCherry from hepatocyte-specific promoter

   Depositor

   Kristin Knouse

   Article

   Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

   Insert

   mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 for sgRNA and hepatocyte-specific promoter (HS…

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiCRISPRv2 EGFP sgRNA2

   Plasmid

   #164933

   Purpose

   Expresses EGFP sgRNA2 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   sgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)
   

   Use

   Lentiviral

   Promoter

   EFS promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ-SpRY-Act3.0

   Plasmid

   #178957

   Purpose

   It consists of a catalytically active SpRY nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL0915 (CRISPR with target 3)

   Plasmid

   #130643

   Purpose

   Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   CRISPR(target-3)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. p8103 LentiCRISPR v2 sgNT-2

   Plasmid

   #163313

   Purpose

   Expresses Cas9 and a non-targeting control guide RNA

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

   Insert

   sgNT-2
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR Human CASP9 -2

   Plasmid

   #198420

   Purpose

   Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

   Depositor

   Hannah Rabinowich

   Article

   Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

   Insert

   Human CASP9 Specific gRNA (CASP9 Human)
   

   Use

   CRISPR

   Tags

   Cas9/Orange Fluorescent Protein Reporter

   Expression

   Mammalian

   Promoter

   U6; CMV

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPR Human CASP9 -1

   Plasmid

   #198419

   Purpose

   Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

   Depositor

   Hannah Rabinowich

   Article

   Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

   Insert

   Human CASP9 Specific gRNA (CASP9 Human)
   

   Use

   CRISPR

   Tags

   Cas9/Orange Fluorescent Protein Reporter

   Expression

   Mammalian

   Promoter

   U6; CMV

   Available Since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiCRISPR v2 RB1 sgRNA #2

   Plasmid

   #202519

   Purpose

   Expressing Cas9 and sgRNA targeting human RB1 gene

   Depositor

   Ming Chen

   Article

   Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

   Insert

   RB1 sgRNA (RB1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPR - EGFP sgRNA 3

    Plasmid

    #51762

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWS5396-CRISPRai-KanR

    Plasmid

    #182835

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - KanR marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. R777-E327 Hs.SPRY1

    Plasmid

    #70611

    Purpose

    Gateway ORF clone of human SPRY1 [NM_001258038.1] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    SPRY1 (SPRY1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIW447-CRISPR XYL2

    Plasmid

    #98906

    Purpose

    K. marxianus CRISPR Plasmid for XYL2 editing

    Depositor

    Ian Wheeldon

    Article

    Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

    Inserts

    Codon optimized Cas9
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. R777-E323 Hs.SPRED2

    Plasmid

    #70607

    Purpose

    Gateway ORF clone of human SPRED2 [NM_181784.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    SPRED2 (SPRED2 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPRv2-TTF-1-gRNA

    Plasmid

    #101857

    Purpose

    Introducing a gRNA targeting human TTF-1 (NKX2-1) into cells

    Depositor

    David Mu

    Article

    Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

    Insert

    gRNA targeting TTF-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. B52 + SPRTN sgSTOP

    Plasmid

    #100709

    Purpose

    B52 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting SPRTN (cloned using BbsI)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoters

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. R777-E329 Hs.SPRY2

    Plasmid

    #70613

    Purpose

    Gateway ORF clone of human SPRY2 [NM_005842.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    SPRY2 (SPRY2 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti CRISPR V2 sgMTHFD1L_1

    Plasmid

    #106316

    Purpose

    Express Cas9 and sgRNA targeting MTHFD1L

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD1L
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. B270 + SPRTN sgSTOP

    Plasmid

    #100718

    Purpose

    B270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting SPRTN (cloned using BbsI)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoters

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pISV-CRISPR/Cas9PGmUbi

    Plasmid

    #209189

    Purpose

    Expression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selection

    Depositor

    Christian Staehelin

    Inserts

    Cas9
    DsRed(deltaB)
    

    Tags

    NLS

    Expression

    Plant

    Mutation

    missing BsaI site and plant-codon optimized with …

    Promoter

    CaMV 35S and GmUbi from Glycine max (L.) Merr. cv…

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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