We narrowed to 4,062 results for: p2a

1. pCAG-RFP-p2a-postASAP

   Plasmid

   #178793

   Purpose

   Expresses voltage sensor targeted to dendrites and spines along with RFP

   Depositor

   Rafael Yuste

   Article

   Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

   Insert

   FingR.PSD95, ASAP2f and TagRFP
   

   Use

   Tags

   TagRFP

   Expression

   Mammalian

   Mutation

   mutations in amino acids L146G, S147T N149R, S150…

   Promoter

   CAG

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV2-GCaMP6f-P2A-PAmCherry

   Plasmid

   #133412

   Purpose

   Fluorescent reporter for calcium imaging and photoactivation.

   Depositor

   Tim Holy

   Article

   Lee et al Science. 2019 Dec 13;366(6471):1384-1389

   Insert

   GCaMP6f-P2A-PAmCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-FNLSHiFiNG-P2A-Puro

   Plasmid

   #136903

   Purpose

   Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSHiFiNG
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Expression

   Mutation

   R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

   Promoter

   EF1s

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

   Plasmid

   #67984

   Purpose

   Cas9 activity reporter with GFP and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Inserts

   U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
   Guide RNA targeting modified BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSIIbleo-iRFP670-P2A-EGFP

   Plasmid

   #198060

   Purpose

   The lentiviral vector for iRFP670 and EGFP

   Depositor

   Michiyuki Matsuda

   Insert

   iRFP670
   

   Use

   Lentiviral

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pINDUCER-21-RUNX1-P2A-ERG

   Plasmid

   #97045

   Purpose

   Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

   Depositor

   George Daley

   Article

   Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

   Insert

   Runt Related Transcription Factor 1, ETS-Related Gene
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet on

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK432 (mAID-P2A-Hygro)

   Plasmid

   #168227

   Purpose

   C-terminal tagging with mAID

   Depositor

   Masato Kanemaki

   Article

   Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

   Insert

   mAID-P2A-Hygro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLC-ZsGreen-P2A-Hygro

   Plasmid

   #124301

   Purpose

   Lentiviral vector for expression of ZsGreen-P2A-Hygro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   ZsGreen
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. PuroR-P2A-DD-TCOF1-donor

   Plasmid

   #60747

   Purpose

   Donor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1

   Depositor

   Benhur Lee

   Article

   Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

   Insert

   TCOF1 (TCOF1 Human)
   

   Use

   Targeting donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

10. ICP8-P2A/Crimson (pSLIK4)

    Plasmid

    #113859

    Purpose

    For Doxycycline-inducible expression of ICP8 synaptase gene, with ICP8-P2A-red fluorescent protein gene E2-Crimson

    Depositor

    Richard S Myers

    Article

    Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

    Insert

    ICP8
    

    Use

    Lentiviral

    Tags

    P2A/Crimson

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-xFNLS-P2A-Puro

    Plasmid

    #110872

    Purpose

    Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xFNLS(3.7)
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-BE4GamRA-P2A-Puro

    Plasmid

    #112673

    Purpose

    Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4GamRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastbacHTB HA-human PP2A Cα D88N

    Plasmid

    #132633

    Purpose

    expresses HA-human PP2A Cα D88N in Hi5 insect cell

    Depositor

    Wenqing Xu

    Article

    Cho et al Nature. 2007 Jan 4;445(7123):53-7. doi:

    Insert

    PP2A Cα D88N
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. BlastR-P2A-DD-TCOF1-donor

    Plasmid

    #60748

    Purpose

    Donor plasmid for HR-mediated recombination to insert blasticidin resistance and append a destabilization domain to the N-terminus of TCOF1

    Depositor

    Benhur Lee

    Article

    Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

    Insert

    TCOF1 (TCOF1 Human)
    

    Use

    Targeting donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pMK433 (mAID-P2A-BSD)

    Plasmid

    #168228

    Purpose

    C-terminal tagging with mAID

    Depositor

    Masato Kanemaki

    Article

    Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

    Insert

    mAID-P2A-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AID-BE4max-P2A-GFP

    Plasmid

    #157948

    Purpose

    Mammalian expression of AID-BE4max-P2A-GFP

    Depositor

    Dali Li

    Article

    Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    AID-BE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-P2A-APEX2-csChBP

    Plasmid

    #108877

    Purpose

    Expression of APEX2 fused to a conditionally stable nanobody against mCherry (includes green cytoplasmic reporter)

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    EGFP-P2A-APEX2-csChBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNOC-CRISPR-P2A-BlastR

    Plasmid

    #98147

    Purpose

    Expresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    P2A-BlastR fusion with Cas9-Nlux

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. mKate2-P2A-APEX2-ML1N*2

    Plasmid

    #67665

    Purpose

    Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-ML1N*2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-nCas9n-P2A-TagBFP2-pA

    Plasmid

    #176026

    Purpose

    Multisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENT

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    nCas9n-P2A-TagBFP2
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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