We narrowed to 8,631 results for: pcDNA3

1. pcDNA3/er-(n2)oxStayGold(c4)

   Plasmid

   #185822

   Purpose

   A soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.

   Depositor

   Atsushi Miyawaki

   Article

   Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

   Insert

   er-(n2)oxStayGold(c4)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 - ciRS-7

   Plasmid

   #169139

   Purpose

   Expression of human CDR1as/ciRS-7 circRNA

   Depositor

   Thomas Hansen, Jørgen Kjems

   Article

   Hansen et al Nature. 2013 Mar 21;495(7441):384-8. doi

   Insert

   CDR1as/ciRS-7 (CDR1-AS Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3_2FLAG-AKAP79ΔCaM

   Plasmid

   #183453

   Purpose

   Expression of human AKAP79 lacking the calmodulin binding site (Δ79-86)

   Depositor

   Matthew Gold

   Article

   Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

   Insert

   AKAP5 (AKAP5 Human)
   

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   aa79-86 deleted

   Promoter

   CMV

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-GR-ICUE2

   Plasmid

   #173017

   Purpose

   Improved green-red FRET based cAMP indicator.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   GR-ICUE2
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-HA-CCND3

   Plasmid

   #172621

   Purpose

   Expresses HA-tagged cyclin D3 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 Foxp1A

   Plasmid

   #16362

   Depositor

   Anjana Rao

   Article

   Hu et al Nat Immunol. 2006 Aug . 7(8):819-26.

   Insert

   FOXP1A (Foxp1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.3TOPO-T7-hCas9

   Plasmid

   #161876

   Purpose

   In vitro transcription of SpCas9

   Depositor

   Stephane Pelletier

   Article

   Pelletier et al Immunity. 2015 Jan 20;42(1):18-27. doi:

   Insert

   human codon optimized SpCas9
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-3C-sGFP11_pcDNA3.1(-)

   Plasmid

   #182932

   Purpose

   For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment

   Depositor

   William Weis

   Article

   Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

   Type

   Empty backbone

   Tags

   3C-cleavable split GFP 11, FLAG tag, and HA signa…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 HA-BACH1

   Plasmid

   #232269

   Purpose

   Expression of N-terminal tagged HA-BACH1 (H. sapiens) in human cell lines.

   Depositor

   Michele Pagano

   Article

   Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

   Insert

   BACH1 (BACH1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3 V5 mDispatched WT 2x HA

    Plasmid

    #126410

    Purpose

    Expression of V5 Dispatched WT HA in mammalian cell lines.

    Depositor

    Stacey Ogden

    Article

    Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

    Insert

    m-Dispatched1 (Disp1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    V5 iserted between A106 and A107

    Available Since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3- SREBP-1

    Plasmid

    #204192

    Purpose

    Mammalian expression of SREBP-1 (isoform 1)

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SREBP-1 (SREBF1 Human)
    

    Tags

    E-tag and T7x2

    Expression

    Mammalian

    Mutation

    multiple silent mutations

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-ER-GCaMP3

    Plasmid

    #64854

    Purpose

    Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CYP450-GCaMP3
    

    Tags

    N-terminal 27 amino acids from CYP450

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1mychisA XDH

    Plasmid

    #10958

    Depositor

    Toren Finkel

    Article

    Ohtsubo et al Circ Res. 2004 Nov 26. 95(11):1118-24.

    Insert

    xanthine dehydrogenase (XDH Human)
    

    Tags

    his and myc

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.3_SARS2_BQ.1.1

    Plasmid

    #194493

    Purpose

    expressing SARS-CoV-2 BQ.1.1 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

    Insert

    Spike (BQ.1.1) (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    R346T, K444T, N460K and c-terminal 18aa del on th…

    Promoter

    CMV

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-MEK5DD-HA

    Plasmid

    #65247

    Purpose

    Expression of HA tagged dominant active MEK5 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

    Insert

    MEK5DD (MAP2K5 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Ser311 to Asp, Ser315 to Asp

    Promoter

    CMV

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-ALK2 wt

    Plasmid

    #80870

    Purpose

    mammalian expression of ALK2 WT

    Depositor

    Aristidis Moustakas

    Article

    Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

    Insert

    ALK2 (ACVR1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-V5-DGCR8

    Plasmid

    #51383

    Depositor

    Narry Kim

    Article

    Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

    Insert

    DGCR8 (DGCR8 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-GAVPO

    Plasmid

    #159923

    Purpose

    GAVPO (VIVID transactivator) expressed under the CMV promoter in the pcDNA3.1 backbone. AKA 133

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    GAVPO
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. mADAM17 in pcDNA3.1

    Plasmid

    #19141

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    ADAM17 (Adam17 Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-HA-eEF2K

    Plasmid

    #110160

    Purpose

    Expression of human eEF2K in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Kruiswijk et al Sci Signal. 2012 Jun 5;5(227):ra40. doi:

    Insert

    eEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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