We narrowed to 8,557 results for: pcDNA3

1. pcDNA3-ER-GCaMP3

   Plasmid

   #64854

   Purpose

   Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CYP450-GCaMP3
   

   Use

   Tags

   N-terminal 27 amino acids from CYP450

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. mADAM17 in pcDNA3.1

   Plasmid

   #19141

   Depositor

   Zena Werb

   Article

   Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

   Insert

   ADAM17 (Adam17 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1(+)- e18

   Plasmid

   #131670

   Purpose

   Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.

   Depositor

   Alberto Ciccia

   Article

   Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

   Insert

   e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_pMagFast2(3x)-iRFP

   Plasmid

   #67297

   Purpose

   Blue light dependent photoswitching system

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

   Insert

   pMagFast2(3x)-iRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-TYMS

   Plasmid

   #100545

   Purpose

   mammalian expression of thymidylate synthetase

   Depositor

   Yuh-Shan Jou

   Article

   Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

   Insert

   thymidylate synthetase (TYMS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Shank2-HA /pCDNA3

   Plasmid

   #197334

   Purpose

   Plasmid expressing an antigen targeting Shank2

   Depositor

   Morgan Sheng

   Insert

   Shank2 (Shank2 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1mychisA XDH

   Plasmid

   #10958

   Depositor

   Toren Finkel

   Article

   Ohtsubo et al Circ Res. 2004 Nov 26. 95(11):1118-24.

   Insert

   xanthine dehydrogenase (XDH Human)
   

   Use

   Tags

   his and myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3/er-(n2)oxStayGold(c4)

   Plasmid

   #185822

   Purpose

   A soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.

   Depositor

   Atsushi Miyawaki

   Article

   Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

   Insert

   er-(n2)oxStayGold(c4)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3.1-H-Luc_nucl

   Plasmid

   #113931

   Purpose

   Plasmid encoding the nuclear localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   H-Luc_nucl
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Twitch-2B pcDNA3

    Plasmid

    #49531

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-2B
    

    Use

    Tags

    ECFP and cpCit174

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-ps-CFP

    Plasmid

    #13034

    Purpose

    Expresses photo-switchable CFP in mammalian cells. Can undergo irreversible photoconversion from cyan to green fluorescence

    Depositor

    Doug Golenbock

    Insert

    photo-switchable cyan fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-N-I-N-CAAX

    Plasmid

    #41686

    Purpose

    Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

    Insert

    Dronpa145N-IntersectinDH-Dronpa145N-CAAX
    

    Use

    Tags

    CAAX box

    Expression

    Mammalian

    Mutation

    K145N on Dronpa

    Promoter

    CMV

    Available since

    Dec. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-p73α-pcDNA3

    Plasmid

    #22102

    Depositor

    William Kaelin

    Article

    Jost et al Nature. 1997 Sep 11. 389(6647):191-4.

    Insert

    tumor protein p73 (TP73 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    Sept. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 TAK1/F

    Plasmid

    #44161

    Depositor

    Xin Lin

    Article

    Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

    Insert

    Tak1 (Map3k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.3_SARS2_BQ.1.1

    Plasmid

    #194493

    Purpose

    expressing SARS-CoV-2 BQ.1.1 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

    Insert

    Spike (BQ.1.1) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R346T, K444T, N460K and c-terminal 18aa del on th…

    Promoter

    CMV

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-Orange_CaMBI_110

    Plasmid

    #124094

    Purpose

    orange calcium-modulated bioluminescent indicator, 110nM

    Depositor

    Michael Lin

    Article

    Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

    Insert

    Orange CaMBI 110
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3 MEKK1 WT

    Plasmid

    #12181

    Depositor

    Gary Johnson

    Article

    Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

    Insert

    MEKK1 (Map3k1 Mouse)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    V1467L

    Promoter

    Available since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3/hArf3(WT)-HA

    Plasmid

    #79414

    Purpose

    Expresses C-terminally HA-tagged Arf3(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

    Insert

    Arf3 (ARF3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-C-Luc

    Plasmid

    #185358

    Purpose

    For mammalian expression of C-terminal half of Firefly luciferase for split-Luciferase experiments (amino acids 398-550).

    Depositor

    Ayaz Najafov

    Insert

    Firefly Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3.1-H-Luc_cyto

    Plasmid

    #113929

    Purpose

    Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    H-Luc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge