We narrowed to 2,579 results for: pcas

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pCASP-SptP120-TD-HilA

Plasmid

#153330

Purpose

Triggers secretion of SptP120 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-HA4-7c12 Tandem Monobody

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP-G3BP2(2)

Plasmid

#136061

Purpose

G3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP2 (G3BP2 Human)

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pACYC-OspCas12c-noncoding

Plasmid

#120878

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-OspCas12

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

Plasmid

#107731

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3

Plasmid

#107728

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-dHeFSpCas9

Plasmid

#92116

Purpose

Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive HeFSpCas9 with FLAG tag

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

Promoter

Cbh

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCaSpeR3/ncd-gfp #10

Plasmid

#66082

Purpose

stable germline transformation of Drosophila to produce Ncd-GFP expression

Depositor

Sharyn Endow

Article

Endow et al J Cell Biol. 1997 Jun 16;137(6):1321-36.

Insert

Ncd kinesin-14 motor protein (ncd Fly)

Tags

GFP

Expression

Insect

Mutation

D699L

Promoter

ncd promoter (3.1kb upstream region of ncd start …

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only
pCaSpeR-act5cB-LexAQFw

Plasmid

#61311

Purpose

Expresses chimeric transactivator LexA_DBD:QF2w_AD in Drosophila

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexA_DBD::QF2w_AD

Expression

Insect

Mutation

Changed EK on C-terminus of QF2 to KKKK

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCas9-EGFP_SLEEK

Plasmid

#249545

Purpose

Insert the Cas9-EGFP into exon 9 of GAPDH for stable expression.

Depositor

Joe Zhang

Article

Zhang et al STAR Protoc. 2025 Jul 4;6(3):103928. doi

Insert

5'arm-Exon9-Cas9-EGFP-3'arm

Use

CRISPR

Tags

EGFP

Promoter

U6 promoter

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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dSpCas9-SmBiT

Plasmid

#236759

Purpose

Split NanoLuc luciferase biosensor fused to dSpCas9

Depositor

David Segal

Article

Heath et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

NanoLuc SmBit

Tags

dSpCas9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG-miniCGBE1

Plasmid

#198538

Purpose

Expresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-miniCGBE1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-NRTH

Plasmid

#198541

Purpose

Expresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRTH

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-NRCH

Plasmid

#198542

Purpose

Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRCH

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase_PCi_PC-σ

Plasmid

#214040

Purpose

Bacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-Pcaspase-Pci-PC-σ

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGGB-SpCas9

Plasmid

#210533

Purpose

Cloning A.thaliana codon optimized SpCas9 CDS as GreenGate module B

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

SpCas9

Use

Synthetic Biology

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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B-HiFi SpCas9

Plasmid

#207362

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HiFi SpCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

R691A, amino acids 1005-1013 replaced with two gl…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-Sniper SpCas9

Plasmid

#207361

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-Sniper SpCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

F539S, M763I, K890N, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-AtSpCas9-9

Plasmid

#197778

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

AtSpCas9

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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SpCas9 dFE

Plasmid

#190137

Purpose

Expresses SpCas9-T4pol (dFE)

Depositor

Timothy Lu

Article

Nakade et al bioRxiv 2022.12.05.518807

Insert

SpCas9-T4pol (dFE)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Codon Optimized for mammalian cells

Promoter

CBh

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Pou5f1

Plasmid

#174872

Purpose

CRISPR vector for generating Pou5f1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Pou5f1 (Pou5f1 Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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