We narrowed to 16,819 results for: IGH@

1. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #112922

   Purpose

   Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETcLIC-GFP

   Plasmid

   #183514

   Purpose

   The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli

   Depositor

   Martin Högbom

   Article

   Grave et al Protein Expr Purif. 2022 Oct;198:106132.

   Type

   Empty backbone

   Use

   Tags

   10x His-tag and folding reporter GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lac

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCOLA-Spec-yibDp-Erv1p

   Plasmid

   #212094

   Purpose

   Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   Sulfydryl oxidase (ERV1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-CMV-TetO2_3C-Twin-Strep

   Plasmid

   #113883

   Purpose

   Mammalian (inducible) protein expression

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKanCMV-mClover3-18aa-Tubulin

   Plasmid

   #74253

   Purpose

   Mammalian expression of N-terminal fusion of mClover3 to Tubulin

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   Tubulin
   

   Use

   Tags

   mClover3

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and CMV

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. p8103 LentiCRISPR v2 sgNT-2

   Plasmid

   #163313

   Purpose

   Expresses Cas9 and a non-targeting control guide RNA

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

   Insert

   sgNT-2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmKate2-MS2coatProtein

   Plasmid

   #103833

   Purpose

   Live cell reader for MS2 loop RNA production

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   mKate2-MS2 coat protein (cp )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

   Promoter

   CMV

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDest-303

   Plasmid

   #159678

   Purpose

   Gateway destination vector for mammalian protein expression

   Depositor

   Dominic Esposito

   Article

   Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p7274 MSCV-P C-FlagHA 16 ∆DLYC

   Plasmid

   #163304

   Purpose

   Expresses HPV16 E7 ∆DLYC

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

   Insert

   HPV16 E7 ∆DLYC
   

   Use

   Retroviral

   Tags

   FlagHA

   Expression

   Mutation

   ∆DLYC

   Promoter

   MSCV LTR

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28_human-pro-IL-18_DeltaN24

    Plasmid

    #214327

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Deletion of first 24 amino acids

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac_human-pro-IL-18

    Plasmid

    #214337

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85866

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-17-3p Sensor (MIR17 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. CVS-N2c-E2_Crimson

    Plasmid

    #172377

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    E2_Crimson
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA FLAG TORC3

    Plasmid

    #22976

    Depositor

    Marc Montminy

    Article

    Screaton et al Cell. 2004 Oct 1. 119(1):61-74.

    Insert

    TORC3 (CRTC3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pPBT-PE2-PGK-Blast

    Plasmid

    #173219

    Purpose

    piggyBac transposon containing PE2 and Blasticidin expression cassettes

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

    Inserts

    PE2
    Blast
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and hPGK

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. xCas9(3.6)-BE3

    Plasmid

    #108385

    Purpose

    Mammalian expression vector for xCas9(3.6)-BE3

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.6)-BE3
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRRL WS1.6 WASp

    Plasmid

    #36250

    Depositor

    David Rawlings

    Article

    Astrakhan et al Blood. 2012 Mar 19.

    Insert

    WASp (WAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    WS1.6

    Available since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGWB545

    Plasmid

    #74879

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. AlkB-D135T

    Plasmid

    #160433

    Purpose

    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr

    Depositor

    Tao Pan

    Article

    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

    Insert

    E. coli AlkB
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 135 to Threonine; deleted t…

    Promoter

    T7

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. RalGDS-YPet

    Plasmid

    #134929

    Purpose

    Fluorescently tagged affinity reagent

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    RaIGDS RBD, YPet
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSIQ-EGFP

    Plasmid

    #216239

    Purpose

    All-in One IQ transgene switch for EGFP expression

    Depositor

    Hyunju Ro

    Article

    Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGWB521

    Plasmid

    #74863

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CVS-N2c-jGCaMP8m

    Plasmid

    #172388

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    GCaMP8m
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHAGE-IRES-puro-NLS-NLS-dHgm4Cas13b-3xGFP-NLS

    Plasmid

    #191368

    Purpose

    Overexpression of NLS-NLS-dHgm4Cas13b-3xGFP-NLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-NLS-dHgm4Cas13b-3xGFP-NLS
    

    Use

    Tags

    3xGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV2-DEST(f)

    Plasmid

    #190226

    Purpose

    Gateway destination vector, empty AAV vector backbone

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-mDLX-TVA-2A-N2cG

    Plasmid

    #172364

    Purpose

    Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from inhibitory neurons

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + B19-N2cG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mDLX

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAGM55297

    Plasmid

    #153213

    Purpose

    Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBS-KLF7-200-GFPfr1

    Plasmid

    #169799

    Purpose

    THe donor vector for the KLF7 gene locus.

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    Donor sequence to KLF7 neighbouring region for HR
    

    Use

    Hr donor vector for human actb gene.

    Tags

    Expression

    Mutation

    Partial sequence of GFP is inserted the sequence …

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LentiCRISPRv2-KLF7-1

    Plasmid

    #169797

    Purpose

    Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    guide RNA for KLF7 neighbouring region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP-N1 SENP6

    Plasmid

    #14583

    Depositor

    Mary Dasso

    Article

    Mukhopadhyay et al J Cell Biol. 2006 Sep 25. 174(7):939-49.

    Insert

    SENP6 (SENP6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2007

    Availability

    Academic Institutions and Nonprofits only