We narrowed to 22,939 results for: REP
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Plasmid#204695PurposeThis plasmid allows for inducible expression of BRD4-NUT(MIN) mutant fusion tagged with mNeonGreen, in mammalian cells.DepositorTagsmNeonGreenExpressionMammalianMutationdeletion of majority of NUT, retaining only amino…PromoterCMVAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only
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pcDNA5-FRT/TO-mNeonGreen-BRD4-p300
Plasmid#204697PurposeThis plasmid allows for inducible expression of engineered BRD4-p300 fusion tagged with mNeonGreen, in mammalian cells.DepositorTagsmNeonGreenExpressionMammalianMutationdirect fusion of BRD4 to p300PromoterCMVAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-Uricase
Plasmid#182417PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-uricase fusionDepositorInsertVNp-mNeongreen-uricase
TagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_mNeongreen-Erythropoietin
Plasmid#182422PurposeBacterial expression of mNeongreen-Erythropoietin fusionDepositorAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Sa-gRNA-puro
Plasmid#191652PurposeLentiviral expression of puromycin resistance gene and S. aureus scrambled non-targeting gRNA ; useful for changing gRNA by mutagenesis.DepositorInsertS. aureus gRNAscr
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-Electra1
Plasmid#179476PurposeER Visualization (OSER assay) Expresses Electra1 in fusion with N-terminal part of p450DepositorInsertCytERM-Electra1
TagsElectra1ExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-EBFP2
Plasmid#184939PurposeER Visualization (OSER assay) Expresses EBFP2 in fusion with N-terminal part of p450DepositorInsertCytERM-EBFP2
ExpressionMammalianPromoterCMVAvailable SinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
MO91-ORAI1N223A-TEV-pHluorin (ORAI1-EC-pHluorin)
Plasmid#174336PurposeThe ORAI1N223A mutant containing-TEV-pHluorin-TEV sequence in the extracellular loop 2DepositorInserthuman Orai1 (ORAI1 Human)
UseRetroviralTagspHluorin inserted in the extracellular loop 2 of …ExpressionMammalianPromoterCMVAvailable SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pG418R-GMRWhite
Plasmid#165883PurposeG418 resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHygroR-GMRWhite
Plasmid#165886PurposeHygromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-ATOH7-T2A-PuroR
Plasmid#162343PurposeLentiviral expression of ATOH7 under the control of the TetON promoterDepositorAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Donor-PIGA
Plasmid#153530PurposeUsed as a donor vector for the targeted correction of a mutation in PIGA exon 6DepositorInsertPIGA (a 1,991-bp fragment from intron 5 and exon 6)
UseDonor plasmidTagsN/AMutationNo mutationPromoterNo promoterAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
R725-X09-635: His6-tev-Hs.SPRED1(13-125)
Plasmid#159581PurposeBaculovirus protein expression of His6-tev-Hs.SPRED1(13-125)DepositorAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Blunt-U6-HES7-STOP-gRNA
Plasmid#130933PurposegRNA for tag human HES7DepositorAvailable SinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBE2QL1-GFP
Plasmid#124667PurposeExpresses UBE2QL1 with C-terminal GFP tag in mammalian cellsDepositorAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_W22HDHFR_WTTS
Plasmid#91241Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationW22HPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_D27NDHFR_WTTS
Plasmid#91244Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationD27NPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31YG121VDHFR_WTTS
Plasmid#91229Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationF31YG121VPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_G121VDHFR_WTTS
Plasmid#91233Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationG121VPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-CXXC/LLAA
Plasmid#124399PurposeFull-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations (TET1 Human)
TagsFlagExpressionMammalianMutationCXXC zinc finger deletion (aa 585-624) and L897A/…Available SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only