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We narrowed to 18,247 results for: multi

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Showing: 6421 - 6440 of 18247 results

  1. AWP-029

    Plasmid
    #213966
    Purpose
    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for gRNA constitutive expression.
    Depositor
    Alexander Westermann
    Publication
    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121
    Insert
    Cas12a (cas12a Segatella bryantii, Synthetic)
    Tags
    3XFLAG
    Expression
    Bacterial
    Mutation
    D875A to inactivate target nuclease activity
    Promoter
    PcfxA, IPTG inducible
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213966-plasmid-map-sequence-id-429164

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  4. pCAT105

    Plasmid
    #180510
    Purpose
    Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Publication
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CasX sgRNAv2
    DpbCasX with R3 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180510-plasmid-map-sequence-id-359734

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  6. pCMU-4

    Plasmid
    #172353
    Purpose
    CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA
    Depositor
    Eriko Takano
    Publication
    Ye et al J Ind Microbiol Biotechnol. 2020 May;47(
    Insert
    D4-TheoR-cas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    D4
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172353-plasmid-map-sequence-id-350007

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  8. pGL2399

    Plasmid
    #137051
    Purpose
    Expression of rapamycin-inducible dimerisation of split Cre from ribosomal locus in Leishmania parasite; pRIB-DiCre
    Depositor
    Jeremy Mottram
    Publication
    Duncan et al Methods Mol Biol. 2019;1971:211-224. doi
    Inserts
    Split Cre Recombinase Rapamycin Inducible Dimerisation
    Blasticidin-S Deaminase
    Use
    Cre/Lox; Leishmania ribosomal locus expression
    Promoter
    For integration into the 18S ribosomal locus
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    137051-plasmid-map-sequence-id-275797

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  10. NLS-LucWT-GFP

    Plasmid
    #127709
    Purpose
    The expressed ORF encodes nucleus location signal with GFP and WT Luciferase.
    Depositor
    Xiaolu Yang
    Publication
    Guo et al J Vis Exp. 2016 Aug 28;(114). doi: 10.37
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Luciferase
    Tags
    EGFP and FLAG-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127709-plasmid-map-sequence-id-248776

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  13. KG#121

    Plasmid
    #110879
    Purpose
    Expresses the C. elegans unc-31 cDNA pan-neuronally
    Depositor
    Kenneth Miller
    Publication
    Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1
    Inserts
    rab-3 promoter
    unc-31 cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Expression
    Bacterial
    Mutation
    Missing first 408bp
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    110879-plasmid-map-sequence-id-212481

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  16. pCFJ1320

    Plasmid
    #84829
    Purpose
    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 introns
    Depositor
    Andrew Fire
    Publication
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized GFP
    Expression
    Bacterial and Worm
    Promoter
    Peft-3
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84829-plasmid-map-sequence-id-163669

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Showing: 6421 - 6440 of 18247 results