We narrowed to 61,606 results for: promoter
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Plasmid#172827PurposeMammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i2 sgRNA / hSpCas9
Plasmid#172826PurposeMammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMU-TPr
Plasmid#61189PurposeFluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under AtUBQ10 promoterDepositorInsertAtTIP1-1
TagsmCherryExpressionPlantPromoterAtUBQ10Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
BLBP-mCherry
Plasmid#63721PurposeExpressing mCherry from BLBP promoter which is a neural stem cell (radial glial cells) specific promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBLBP (Fabp7 Mouse)
ExpressionMammalianMutation1700 bp upstream of BLBP gene cloned in CAG-mCher…Promoter1700bp upstream of BLBP geneAvailable sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL001
Plasmid#115674PurposeFluorescent reporter for E.coli K12 fliC promoter activityDepositorInsertfliC promoter fused with GFP
TagsGreen fluorescent protein (GFP)ExpressionBacterialPromoterfliC promoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBADTrfp
Plasmid#99382PurposeThis promoter system provided the highest RFP expression upon inductionDepositorInsertRFP
ExpressionBacterialAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LentiGuide Cherry
Plasmid#170510PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.DepositorPublicationInsertsS. pyogenes sgRNA cassette
mCherry
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1a and hU6Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRRlsinPGK_H2BGFP_WPRE
Plasmid#91788PurposeConstitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.DepositorAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine
Plasmid#50468PurposeGs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoterDepositorInsertrM3D-IRES-mCitrine
UseAAVTagsHAMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti.Syn(0.5).H2B-eGFP.W
Plasmid#51004PurposeLentivector that expresses H2B-eGFP from neurons-specific synapsin promoterDepositorInsertH2B-EGFP (H2BC11 Jellyfish, Human, Synthetic)
UseLentiviralTagshistone2BExpressionMammalianPromoterrat synapsinAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMU-PDESr
Plasmid#61206PurposeFluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoterDepositorInsertAtPDLP1
TagsmCherryExpressionPlantPromoterAtUBQ10Available sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pMXs-IG
Plasmid#51258PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-EGFP-ELYS-polyA
Plasmid#59746PurposeExpress EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP-ELYS
TagsEGFPExpressionMammalianPromoterCMV and T7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMX-HA-UbvG08_MUT-IRES-GFP
Plasmid#75031PurposeRetroviral vector with ubiquitin variant with reverted mutations L69P and V70L (i53-DM mutant)DepositorInsertUbiquitin
UseRetroviralTagsHA and IRES-eGFPMutationUbvG08 with I44A, P69L, and L70V mutations and no…Available sinceJune 30, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDH-CB-FLPe-P2A-tdTomato
Plasmid#72259PurposeExpress FLPe and tdTomato under CB promoterDepositorInsertFLPe-P2A-tdTomato
UseLentiviralExpressionMammalianMutationT230I in tdTomato (please see depositor's co…PromoterCB promoter and chicken beta-actin + CMV enhancerAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOT2
Plasmid#14460DepositorInsertGfp-UV
ExpressionBacterialAvailable sinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti.CAG.H2B-cerFP-2A-mito-dsRFP.W
Plasmid#51013PurposeLentivector that expresses H2B-cerFP-2A-mito-dsRFP from internal CAG enhancer-promoterDepositorInsertH2B-cerFP-2A-mito-RFP
UseLentiviralExpressionMammalianPromoterCAGAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSL0284 (pQCascade_entry)
Plasmid#130635PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-pCALM1-EYFP-WPRE-bGHpA
Plasmid#213971PurposeAAV expression of EYFP from pCALM1 promoterDepositorInsertpCALM1
UseAAVAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+(rInsp)-Ins-GLuc
Plasmid#89928PurposeExpresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman insulin-Gaussia-Luciferase (INS Human, Synthetic)
ExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…Promoterrat Insulin II promoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by