We narrowed to 17,662 results for: por
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Plasmid#158665PurposeThis plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combinationDepositorTypeEmpty backboneUseLentiviralTagshcREDExpressionMammalianAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only
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pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry
Plasmid#177183Purposeplasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry markerDepositorInsertsgRNA targeting the PEAR-GFP plasmid
UseCRISPRExpressionMammalianPromoterU6Available SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xflag-APEX2-NES
Plasmid#164623PurposeExpresses a nuclear exporting signal (NES) tagged with APEX2 and 3xflagDepositorInsertNES
Tags3xFLAG and APEX2ExpressionMammalianPromoterCMVAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP9A(E195Q)-HA
Plasmid#209244PurposeMammalian expression of ATP9A mutant E195QDepositorAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-ChR2-Kv-P2A-H2b-mRuby2
Plasmid#89570PurposeChannelrhodopsin localized to neuronal soma with simultaneous nuclear labelingDepositorInsertsChannelrhodopsin
Histone 2B
UseAAVTagsmRuby2ExpressionMammalianMutationH134RPromotersynapsinAvailable SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330 EGFR-sgRNA
Plasmid#188633PurposeA human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFRDepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRXIB-mRuby3-STX17TM
Plasmid#221744PurposeStable expression of mRuby3-STX17TM in mammalian cellsDepositorInsertSTX17 (STX17 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationdeleted amino acids 1-228Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXs_NMNAT3-FLAG
Plasmid#133261PurposeExpress FLAG-tagged NMNAT3 in mammalian cellsDepositorAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL
Plasmid#216253PurposeFRET-based calcium sensor located in the ER lumen, Tet-inducibleDepositorInsertER-targeted D4 second generation cameleon (FRET calcium sensor)
UseLentiviralTagsREAch and mTurquoisePromoterTRE3GAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTW072M
Plasmid#115960PurposeDestination VectorDepositorInsertLvl2 (nsP2 Q739L)
UseSynthetic BiologyMutationnsP2 Q739LAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-MyosinVb-Ctail
Plasmid#110170PurposeDominant negative effect by competing with MyosinVb C-terminal tail interactionsDepositorInsertMyosin Vb (Myo5b Rat)
TagsEGFPExpressionMammalianMutationamino acids 1262-1670PromoterCMVAvailable SinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai
Plasmid#202571PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
TagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Methionine 91 to Alanine, ORF1p Glu…Available SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-FlicR1
Plasmid#74143PurposeExpress FlicR1 preferentially in neuronsDepositorInsertFlicR1
UseAAVExpressionMammalianPromoterhSynAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGREAT2
Plasmid#170890PurposeGoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_NOSt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…Promoterp35SoTMVAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP
Plasmid#45579PurposeExpresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP trackerDepositorInsertcoY2 Ani K227M
UseLentiviralTagsHA, IRES BFP, and NLSExpressionMammalianMutationco indicates codon optimization for mammalian exp…PromoterSFFVAvailable SinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
GB4542
Plasmid#215261PurposeModule for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19DepositorInsertCBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4401
Plasmid#215252PurposeModule for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.DepositorInsertSF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPC019.AAV
Plasmid#171147PurposeExpression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmidDepositorInsertshAAVS1 scRNA
J1-BBa_J23117-mRFP
UseCRISPRExpressionBacterialPromoterBBa_J23119 and J1-BBa_J23117Available SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTD-C_sfYFPTwinStrep
Plasmid#45943Purposeexpresses P.putida codon optimized super-folder sfYFP-Twin-Strep fusion proteinDepositorInsertsynthetic sfYFP (codon usage adapted to P.putida KT2440)
TagsTwin-Strep-tagExpressionBacterialPromoterlacIq/PtrcAvailable SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-tagBFP-PSM-EGFP
Plasmid#100603PurposeDonor scaffold with EGFP positive selection module, and tagBFP negative selection moduleDepositorTypeEmpty backboneUseSynthetic BiologyTagsEGFP-T2A-Puro and tagBFPAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only