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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,522 results for: sas;

Showing: 6461 - 6480 of 6522 results

  1. DBDmut-CA-RIT-NFAT1

    Plasmid
    #63669
    Purpose
    Constitutively active NFAT1 and also unable to interact with AP-1 with four point mutations in the DNA binding loop that abolish DNA binding
    Depositor
    Anjana Rao
    Article
    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15
    Insert
    NFAT1 (Nfatc2 Mouse)
    Use
    Retroviral
    Tags
    3x HA
    Expression
    Mammalian
    Mutation
    CA: PASSGSSASF mutated to PAAAGAAAAF, SPRTSPIMSP…
    Promoter
    Available Since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. pWZL-N1ICD-Flag

    Plasmid
    #171171
    Purpose
    Retroviral vector for expression of NOTCH1 intracellular domain with a C-terminal FLAG tag (to be used in conjunction with Phoenix packaging cells).
    Depositor
    Masashi Narita
    Article
    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do
    Insert
    NOTCH1 intracellular domain (NOTCH1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Will express residues 1758-2556 of human NOTCH1, …
    Promoter
    Viral LTR
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    171171-plasmid-map-sequence-id-338649

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  3. pLPC-JAG1-mVenus

    Plasmid
    #171170
    Purpose
    Retroviral vector for CMV promoter driven expression of JAG1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).
    Depositor
    Masashi Narita
    Article
    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do
    Insert
    JAG1 (JAG1 Human)
    Use
    Retroviral
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    171170-plasmid-map-sequence-id-339658

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  4. pT/CAGGS-mVenus-P2A-NRASG12V

    Plasmid
    #236072
    Purpose
    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and mutant NRAS (NRASG12V).
    Depositor
    Masashi Narita
    Article
    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:
    Inserts
    mVenus
    NRAS G12V (NRAS Human)
    Use
    Sleeping beauty transposon plasmid
    Tags
    Expression
    Mutation
    G12V
    Promoter
    CAGGS
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236072-plasmid-map-sequence-id-472895

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  5. pBFC0996

    Plasmid
    #186695
    Purpose
    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    crRNA
    Tn7 transposon
    2xLguI Cargo Stuffer
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLac
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186695-plasmid-map-sequence-id-371382

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  6. pSbi-pur-EGFP-KSR1-CA3 (N-KSR)

    Plasmid
    #217769
    Purpose
    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)
    Depositor
    Paula Nunes-Hasler
    Article
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Sleeping beauty transposase-compatible genome ins…
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CA3 domain aa 317-400
    Promoter
    EF-1alpha promoter
    Available Since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217769-plasmid-map-sequence-id-435786

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  7. pSbi-pur-KSR1-CA3-EGFP (C-KSR)

    Plasmid
    #217768
    Purpose
    Fluorescent reporter for ceramide (for stable mammalian expression)
    Depositor
    Paula Nunes-Hasler
    Article
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Sleeping beauty transposase-compatible genome ins…
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CA3 domain aa 317-400 plus vector-based linker LE…
    Promoter
    EF-1alpha promoter
    Available Since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217768-plasmid-map-sequence-id-435794

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  8. Spike Display_Part 2 Spacer

    Plasmid
    #172730
    Purpose
    Encodes Part 2 of HexaPro-D614G spike to be used in the Spike Display system
    Depositor
    IIya Finkelstein
    Article
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    172730-plasmid-map-sequence-id-340023

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  9. 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

    Plasmid
    #169168
    Purpose
    Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.
    Depositor
    Sascha Martens
    Article
    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017
    Insert
    MAP1LC3B (MAP1LC3B Human)
    Use
    Tags
    6X Histidine Tag, TEV cleavage site, mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac promoter
    Available Since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169168-plasmid-map-sequence-id-332048

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  10. pT/UBC-mVenus-P2A-NRASG12V

    Plasmid
    #236074
    Purpose
    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Ubc promotor driven expression of mVenus and mutant NRAS (NRASG12V).
    Depositor
    Masashi Narita
    Article
    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:
    Inserts
    mVenus
    NRAS G12V (NRAS Human)
    Use
    Sleeping beauty transposon plasmid
    Tags
    Expression
    Mutation
    G12V
    Promoter
    Ubc
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236074-plasmid-map-sequence-id-472894

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  11. pT/PGK-mVenus-P2A-NRASG12V

    Plasmid
    #236073
    Purpose
    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Pgk promotor driven expression of mVenus and mutant NRAS (NRASG12V).
    Depositor
    Masashi Narita
    Article
    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:
    Inserts
    mVenus
    NRAS G12V (NRAS Human)
    Use
    Sleeping beauty transposon plasmid
    Tags
    Expression
    Mutation
    G12V
    Promoter
    PGK
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236073-plasmid-map-sequence-id-472890

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  20. pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A/W276A/I279A/I307A/V310A; deltaLIR1+3+4)

    Plasmid
    #223784
    Purpose
    Expression of recombinant protein for purification
    Depositor
    Sascha Martens
    Article
    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038
    Insert
    BCL2L13 soluble part (BCL2L13 Human)
    Use
    Tags
    EGFP-TEV-GST
    Expression
    Bacterial
    Mutation
    I224A/L227A/W276A/I279A/I307A/V310A; deletion of …
    Promoter
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    223784-plasmid-map-sequence-id-448415

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Showing: 6461 - 6480 of 6522 results