We narrowed to 8,596 results for: Set

1. miR21-IRE-TREAT

   Plasmid

   #196930

   Purpose

   mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.

   Depositor

   Jeffrey Chao

   Article

   Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

   Insert

   Renilla luciferase
   

   Expression

   Bacterial and Mammalian

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Let7-IRE-TREAT

   Plasmid

   #196925

   Purpose

   Let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.

   Depositor

   Jeffrey Chao

   Article

   Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

   Insert

   Renilla luciferase
   

   Expression

   Bacterial and Mammalian

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTK678_002

   Plasmid

   #123923

   Purpose

   Encodes the TU GFP dropout cassette with Ampicillin resistance and bacterial artificial chromosome as a type 678 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   BAC-AmpR
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-DSCP-Gateway

   Plasmid

   #71507

   Purpose

   flySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the DSCP core promoter

   Depositor

   Alexander Stark

   Article

   Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

   Type

   Empty backbone

   Use

   Flystarr-seq validation vector

   Tags

   luciferase

   Expression

   Insect

   Available Since

   Dec. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS B2rescue A70D

   Plasmid

   #78658

   Purpose

   Generates B2 inserts suitable for rescue

   Depositor

   Astrid Roy-Engel

   Article

   Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

   Insert

   B2rescue cassette
   

   Expression

   Mammalian

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Myog'-2A-mCherryNLS-PGK-Purodtk

   Plasmid

   #69547

   Purpose

   Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

   Depositor

   Charles Gersbach

   Inserts

   2A-mCherry-NLS
   Myogenin 5' homology arm (Myog Mouse)
   Myogenin 3' homology arm (Myog Mouse)
   Puro-delta-tk
   

   Use

   Mouse Targeting

   Tags

   2x nuclear localization signal and PVT1-2A "…

   Mutation

   Silent V205V mutation in myogenin gene to prevent…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLib6.4B

   Plasmid

   #176665

   Purpose

   Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLD-puro-PGKnVA

   Plasmid

   #24589

   Depositor

   Jason Moffat

   Article

   Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

   Insert

   Gateway(TM) cassette
   

   Use

   Lentiviral

   Tags

   VA

   Expression

   Mammalian

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

9. MXS_PGK::NeoR-bGHpA

   Plasmid

   #62438

   Purpose

   MXS_chaining vector with PGK::NeoR-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   resistance cassette against Neomycin with PGK Promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. CpcB-Solanum-lycopersicumPHLS_CpcA

    Plasmid

    #74003

    Purpose

    Expresses the Solanum lycopersicum PHLS as a fusion with the CpcB protein plus the NPPS and CmR cassete in Synechocystis

    Depositor

    Anastasios Melis

    Article

    Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

    Inserts

    PHLS and CmR
    Neryl diphosphate synthase from Solanum lycopersicum
    

    Promoter

    cpc

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCXLE-dCas9GFP-shP53

    Plasmid

    #102906

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9GFP-shP53
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLib6.6B

    Plasmid

    #176666

    Purpose

    Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSTBlue-U6-sgRNA(tdTomato)

    Plasmid

    #47871

    Purpose

    This plasmid contains expression cassette for sgRNA targeting tdTomato.

    Depositor

    Erik Sontheimer, James Thomson

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Insert

    sgRNA-tdTomato
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLS.546

    Plasmid

    #184963

    Purpose

    Test effect of a1/a2 length on editing priB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 22
    

    Expression

    Bacterial

    Mutation

    priB donor G1069A, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Let7R-IRE-TREAT

    Plasmid

    #196934

    Purpose

    Reverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT.

    Depositor

    Jeffrey Chao

    Article

    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

    Insert

    Renilla luciferase
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDYSCKO

    Plasmid

    #120263

    Purpose

    Empty backbone for cloning any gRNA to be used with the canavanine co-selection system

    Depositor

    Christian Landry

    Article

    Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

    Insert

    DYSCKO cassette
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    SNR52

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLS.540

    Plasmid

    #184959

    Purpose

    Test effect of a1/a2 length on editing fabH using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 22
    

    Expression

    Bacterial

    Mutation

    fabH donor G954A, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIE2-AD

    Plasmid

    #21603

    Depositor

    Takahiro Kusakabe

    Article

    Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

    Insert

    NF-kappa-B p65 subunit (Rela Mouse)
    

    Use

    Gateway destination vector

    Tags

    Flag, Gateway destination cassette, and NLS

    Expression

    Insect

    Available Since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTL472

    Plasmid

    #69882

    Purpose

    dCirl promoter region was replaced with an optimized 20xUAS-IVS promoter cassette

    Depositor

    Robert Kittel, Tobias Langenhan

    Article

    Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

    Insert

    CIRL (Cirl Fly)
    

    Use

    Transformation vector

    Tags

    FLAG

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-STOP-shA1

    Plasmid

    #14422

    Depositor

    Klaus Rajewsky

    Article

    Oberdoerffer et al Mol Cell Biol. 2005 May . 25(10):3896-90

    Insert

    modified U6 promoter (U6lox), U6 STOP cassette, shA1 gene
    

    Use

    Cre/Lox and RNAi

    Expression

    Mammalian

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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