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nDHFR173-gs10-NLS-VPR

Plasmid

#188262

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split DHFR (aa 174-186), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nDHFR173 (DHFR Human)

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-neo-myc-LC3(deltaC22)

Plasmid

#45448

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

myc

Expression

Mammalian

Mutation

Encodes amino acids 1-120

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
JPF0535a

Plasmid

#124042

Purpose

Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-31SM-PGK-GFP-Blast

Plasmid

#31516

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-31 (Mir31 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

seed mutant control

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pB2AR-miRFP670nano

Plasmid

#127442

Purpose

Beta-2-adrenergic receptor internally tagged with miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

B2AR-miRFP670nano (ADRB2 Synthetic, Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pX330 NC-FKBP-Cas9

Plasmid

#162725

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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SPRR2A-pFastBac I

Plasmid

#182460

Purpose

Expression His-tagged SPRR2A in insect cells

Depositor

Lora Hooper

Article

Hu et al Science. 2021 Nov 5;374(6568):eabe6723.

Insert

small proline rich protein 2A (SPRR2A Human)

Tags

6xHis

Expression

Insect

Promoter

Polyhedrin

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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Frt-hspB6

Plasmid

#63097

Purpose

Expression of HSPB6 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb6 (HSPB6 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pSD36_pCAG-PV1-FKBP-L1-TP901-327C-BGHpA

Plasmid

#108814

Purpose

Expresses aa327 to C-terminus of TP901 integrase fused to FKBP

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

TP901 integrase fragment aa327 to C-terminus

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Promoter

pCAG

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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HA-HIF1a (S668A)

Plasmid

#49463

Purpose

HA-tagged HIF-1a Ser668 resuide mutated to Ala

Depositor

Wafik El-Deiry

Article

Warfel et al Cell Cycle. 2013 Oct 25;12(23).

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 668 to Alanine

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only
FLAG-MED9

Plasmid

#15407

Depositor

Joan Conaway, Ronald Conaway

Article

Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

Insert

MED9 (Med9 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pLenti GW-mCit-PA

Plasmid

#113457

Purpose

Lentiviral ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Lentiviral

Tags

mCitrine-ProteinA

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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Rtn4a-NTD1-135-mNeon

Plasmid

#186609

Purpose

Encodes Reticulon 4 N-terminal domain aa 1 to 135 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 4 isoform A amino acids 1 to 135 (RTN4 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Frt-hspB4

Plasmid

#63095

Purpose

Expression of HSPB4 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb4 (CRYAA Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-iCre-pA]-lox-Lyn-EGFP

Plasmid

#196875

Purpose

Neuron-specific expression of LynGFP reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

LynEGFP

Use

Cre/Lox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pClneoMyc MOB1 T74A

Plasmid

#47939

Purpose

expresses Myc tagged human MOB1 containing T74A mutation

Depositor

Yutaka Hata

Article

Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

Insert

MOB1A (MOB1A Human)

Tags

Myc

Expression

Mammalian

Mutation

T74A

Promoter

CMV

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9

Plasmid

#163644

Purpose

Makes a lentivirus that expresses mNeonGreen and Cas9.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

mNeonGreen-P2A-Cas9

Use

Lentiviral

Tags

mNeonGreen-P2A

Promoter

EFS

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti GW-NL-myc

Plasmid

#113455

Purpose

Lentiviral NanoLuc Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Lentiviral and Luciferase

Tags

NanoLuc-cmyc

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pX330 L-FKBP-Cas9

Plasmid

#162728

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBW2549_pCAG-PV1-VCre-N269-L1-GID1-NLS-BGHpA

Plasmid

#108773

Purpose

Expresses N-terminus to aa269 of VCre recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

VCre recombinase fragment N-terminus to aa269

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2569_pCAG-PV1-GAI-L1-VCre-270C-NLS-BGHpA

Plasmid

#108774

Purpose

Expresses aa270 to C-terminus of VCre recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

VCre recombinase fragment aa270 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/SECFP-mATG5

Plasmid

#58744

Purpose

Expresses /SECFP-ATG5 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

autophagy related genes 5 (Atg5 Mouse)

Use

Retroviral

Tags

SECFP

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
pAT15542_nCBE-SuperFi-Cas9

Plasmid

#184372

Purpose

Mammalian expression of SuperFi-Cas9 CBE base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

nCBE-SuperFi-Cas9

Use

CRISPR

Tags

3X FLAG, SV40 NLS, and UGI

Expression

Mammalian

Mutation

D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

Promoter

EF-1α core promoter

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAT15544_nABE-SuperFi-Cas9

Plasmid

#184374

Purpose

Mammalian expression of SuperFi-Cas9 ABE7 base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

nABE-SuperFi-Cas9

Use

CRISPR

Tags

FLAG, SV40 NLS, and nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

Promoter

EF-1α core promoter

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCOCK CM-R03

Plasmid

#46567

Purpose

Mammalian expression plasmid with EGFP expressed from synthetic promoter (random mutagenesis of CMVwt 3' end).

Depositor

Clifford Wang

Article

Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.

Insert

CMV mutant promoter

Use

Synthetic Biology

Expression

Mammalian

Mutation

Mutant version of CMV promoter (refer to Table 1 …

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only
lenti-sgRNA(MS2)_zeo_hPDX1_promoter_guide1

Plasmid

#176838

Purpose

To induce human PDX1 expression by recruiting SAM complex to PDX1 promoter

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

sgPDX1

Expression

Mammalian

Promoter

U6

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-NES-tdTomato-LINuS-WPRE

Plasmid

#159894

Purpose

AAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signal

Depositor

Tatsushi Onaka

Article

Inutsuka et al Biochem Biophys Res Commun. 2020 Jan 29;

Insert

NES-tdTomato-LINuS

Use

AAV

Expression

Mammalian

Promoter

EF1α promoter

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pClneoMyc MOB1 T12A

Plasmid

#47936

Purpose

expresses Myc tagged human MOB1 containing T12A mutation

Depositor

Yutaka Hata

Article

Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

Insert

MOB1A (MOB1A Human)

Tags

Myc

Expression

Mammalian

Mutation

T12A

Promoter

CMV

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pJZC77

Plasmid

#62338

Purpose

sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA
COM-KRAB

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…

Promoter

CMV and U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pMinDis.X513-iNicSnFR3b-(V7)-PM

Plasmid

#125124

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Article

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR3b-(V7)-PM

Tags

Ig-K-Chain and membrane anchoring protein-PDGFR

Expression

Mammalian

Promoter

T7

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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