We narrowed to 7,672 results for: CCH

1. pGS1021

   Plasmid

   #59575

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection marker

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
   

   Use

   E. coli cloning vector

   Tags

   Twin-Strep tag

   Promoter

   None

   Available Since

   Aug. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs_FLAG-GGC1

   Plasmid

   #133258

   Purpose

   Express FLAG-tagged GGC1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   GGC1 (GGC1 Budding Yeast)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in human cells

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. DT049A

   Plasmid

   #159410

   Purpose

   Constitutive murine mature BDNF expression, controlled under E.coli tet promoter

   Depositor

   Sheref Mansy

   Article

   Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

   Insert

   pr.tet-BDNF (Bdnf, Mouse) (Bdnf Synthetic, Mouse)
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Bacterial

   Promoter

   tet promoter

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGAN201

   Plasmid

   #129204

   Purpose

   yTRAP sensor of [RNQ+], detects aggregation of Rnq1

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   RNQ1 (RNQ1 Synthetic, Budding Yeast)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   SUP35

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEasyG1_hph

   Plasmid

   #184912

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p406 – TDH3-Elo3-GFP11-Ura

   Plasmid

   #177734

   Purpose

   ER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA marker

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   Elo3 (ELO3 Budding Yeast)
   

   Tags

   GFP11 and HDEL

   Expression

   Bacterial and Yeast

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs_FLAG-NDT1

   Plasmid

   #133254

   Purpose

   Express FLAG-tagged NDT1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   NDT1 (YIA6 Budding Yeast)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in human cells

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM6-RgTALsyn-HpaBC

   Plasmid

   #100949

   Purpose

   Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Inserts

   RgTALsyn
   HpaB
   HpaC
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   T7

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-eGFP-SynaptoZip

   Plasmid

   #122525

   Purpose

   AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

   Depositor

   Antonio Malgaroli

   Article

   Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

   Insert

   eGFP-SynaptoZip (Vamp2 Rat)
   

   Use

   AAV

   Tags

   Myc and eGFP

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWzyE-distrepII

    Plasmid

    #196667

    Purpose

    Expression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzyE
    

    Tags

    TEV-cleavable di-strep tag II

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETDuet-1 TIM8,13

    Plasmid

    #170282

    Purpose

    Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea

    Insert

    yeast Tim8 and Tim13 (TIM8 Budding Yeast)
    

    Tags

    TEV-cleavable N-terminal His tag on Tim13 (on bac…

    Expression

    Bacterial

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET151-Vps4(101-437)-Hcp1

    Plasmid

    #87737

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100

    Promoter

    T7

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pST1374-D10A-GCN4

    Plasmid

    #113025

    Purpose

    Expresses D10A-GCN4 in mammalian cells

    Depositor

    Xingxu Huang

    Article

    Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

    Insert

    D10A-GCN4 (GCN4 Budding Yeast, S. pyogenes)
    

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMV

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBBK67 pHomL-RPL18Bp-RPC19-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179051

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Scarlet(rfp), a marker for the nucleolus

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-RPC19-Link-Scarlet(rfp)
    

    Expression

    Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. YIp128-CUP1-NUbo-GFP-His6

    Plasmid

    #212790

    Purpose

    Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-GFP-His6
    

    Use

    Integrative vector

    Expression

    Yeast

    Promoter

    CUP1

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIMVAd39T

    Plasmid

    #98303

    Purpose

    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    pdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

    Expression

    Yeast

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAEL007097-Cas9

    Plasmid

    #100705

    Purpose

    The promoter of AAEL007097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL007097-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Promoter

    AAEL007097 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGAN200

    Plasmid

    #129203

    Purpose

    yTRAP sensor of [PSI+], detects aggregation of Sup35

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    Sup35NM (SUP35 Budding Yeast)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    N terminal and middle domains

    Promoter

    SUP35

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFL-EGFP-3xFlag-Fml1

    Plasmid

    #193058

    Purpose

    Baculovirus expression of S.pombe Fml1 for recombinant protein production

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    Fml1 (fml1 Fission Yeast)
    

    Tags

    3xFlag

    Expression

    Insect

    Promoter

    polyhedron

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

    Plasmid

    #204288

    Purpose

    yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pHIS3p:yomRuby3-Tub1+3'UTR::LEU2
    

    Tags

    mRuby3

    Expression

    Yeast

    Promoter

    pHis3

    Available Since

    July 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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