We narrowed to 21,797 results for: Sis

1. pAB094a

   Plasmid

   #79241

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PBAD rpoZ-HA4; PC araC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD; PC

   Available since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-Zeo-mEGFP

   Plasmid

   #214145

   Purpose

   Expresses the monomeric EGFP in eukaryotic cells

   Depositor

   Bertrand Collet

   Article

   Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

   Insert

   mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A207K L221K F223R

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGene-TAP-GBP1

   Plasmid

   #138087

   Purpose

   Plasmid with TAP-GBP1 ORF

   Depositor

   Eva Frickel

   Article

   Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

   Insert

   human GBP1 (GBP1 Human)
   

   Use

   Tags

   Tandem affinity purification (TAP) tag

   Expression

   Mammalian

   Mutation

   Promoter

   Gal4-E1b

   Available since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. VAMP2-pHuji

   Plasmid

   #105289

   Purpose

   VAMP2 tagged with pH-sensitive red fluorescent protein pHuji

   Depositor

   Justin Taraska

   Article

   Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

   Insert

   VAMP2 (Vamp2 Rat)
   

   Use

   Tags

   pHuji

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. OaAEP1_C247A

   Plasmid

   #200307

   Purpose

   Bacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)

   Depositor

   Artur Krężel

   Article

   Antonenko et al Bioconjug Chem. 2023 Mar 15;34(4):719-27

   Insert

   Asparaginyl endopeptidase from Oldenlandia affinis
   

   Use

   Tags

   Smt3

   Expression

   Bacterial

   Mutation

   Cysteine 247 to Alanine

   Promoter

   T7

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCRISPR V2 sgIREB2_2

   Plasmid

   #169886

   Purpose

   Disrupt IREB2

   Depositor

   Richard Possemato

   Article

   Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

   Insert

   sgRNA targeting IREB2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEn-Chimera

   Plasmid

   #61432

   Purpose

   Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   AtU6-26:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLBM0010

   Plasmid

   #172816

   Purpose

   Level 2 module containing HSP21-GFP

   Depositor

   Benjamin Field

   Article

   Velay et al Plant Methods. 2022 May 26;18(1):69. doi

   Insert

   [35S-P19][35S-CTPSSU-mHSP21-GFP]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Flag-cIAP1/pRK5

   Plasmid

   #27972

   Depositor

   Xiaolu Yang

   Article

   Hu et al J Biol Chem. 2003 Mar 21;278(12):10055-6

   Insert

   CIAP1 (BIRC2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-Neo-LUC

    Plasmid

    #214368

    Purpose

    Expression of the firefly luciferase Protein

    Depositor

    Bertrand Collet

    Article

    Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

    Insert

    LUC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDe-CAS9

    Plasmid

    #61433

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PcUBI4-2

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK1043

    Plasmid

    #8835

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    tobacco etch virus protease, catalytic domain
    

    Use

    Tags

    MBP and polyarginine

    Expression

    Bacterial

    Mutation

    Autolysis-resistant S219V mutant

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-Smac(1-60)mCherry

    Plasmid

    #40880

    Depositor

    Douglas Green

    Article

    Tait et al Dev Cell. 2010 May 18;18(5):802-13.

    Insert

    Smac(1-60)mCherry (DIABLO Human)
    

    Use

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pBlu/gRNA

    Plasmid

    #59188

    Purpose

    Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

    Depositor

    Robert Stupar

    Article

    Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

    Insert

    Guide RNA Cassette
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    U6 (Arabidopis)

    Available since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKM427

    Plasmid

    #210788

    Purpose

    L5 integrating vector for delivery of defective Hyg gene (ZeoR) for oligonucleotide-mediated SNP transfer

    Depositor

    Kenan Murphy

    Article

    Murphy et al Methods Mol Biol. 2021;2314:301-321. doi

    Insert

    Defective hygromycin resistant gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed hygromycin resistant gene W190 codon (TGG…

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAC-ZEAX

    Plasmid

    #53274

    Purpose

    Contains crtE, crtB, crtI, crtY and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces zeaxanthin in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Sun et al J Biol Chem. 1996 Oct 4;271(40):24349-52

    Insert

    crtE, crtY, crtI, crtB, crtZ
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Deleted idi (ORF6) and crtX genes

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXEE401BG

    Plasmid

    #91717

    Purpose

    CRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJYS1Peftu

    Plasmid

    #85546

    Purpose

    Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    recT
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    etfu and unknown

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.1P shACO1

    Plasmid

    #169884

    Purpose

    Suppress ACO1

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    shRNA against ACO1
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Tom20-CIB-GFP

    Plasmid

    #117242

    Purpose

    Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch

    Depositor

    Robert Hughes

    Article

    Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

    Insert

    CIBN
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDe-CAS9-D10A

    Plasmid

    #61477

    Purpose

    based on pDe-CAS9, encodes Cas9 D10A nickase variant

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    Cas9-D10A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    catalytic Asp10 changed to Ala

    Promoter

    PcUbi4-2

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJV53-Cas12a

    Plasmid

    #158706

    Purpose

    CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAS9-TPC

    Plasmid

    #61478

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 for conventional cloning

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PcUbi4-2

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti-CMVSP6-nEGFP-SV40-PURO

    Plasmid

    #138364

    Purpose

    Lentiviral plasmid for nuclear EGFP expression driven by CMVSP6 and puromycin resistance under SV40 promoter for selection

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    nuclear EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSW610 - GR-LhG4_BD

    Plasmid

    #115992

    Purpose

    entry vector for GreenGate cloning method, CDS, with adaptors for B and D modules

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    GR-LhG4_BD
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSW181 - GR-LhG4

    Plasmid

    #115983

    Purpose

    entry vector for GreenGate cloning method, CDS

    Depositor

    Jan Lohmann

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    GR-LhG4
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PTEN shRNA

    Plasmid

    #86645

    Purpose

    expression of shRNA targeting PTEN

    Depositor

    Jaime Modiano

    Article

    Lin et al Mol Cancer. 2007 Feb 14;6:16.

    Insert

    PTEN (PTEN Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. B52_puro_empty_gRNA

    Plasmid

    #197557

    Purpose

    sites for cloning two gRNA with puromycin cassette

    Depositor

    Darius Bägli

    Type

    Empty backbone

    Use

    Empty grna backbone with puromycin resistance gene

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for gRNA, CMV for puromycin resistance.

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLentiCRISPR V2 sgIREB2_1

    Plasmid

    #169894

    Purpose

    Disrupt IREB2

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    sgRNA targeting IREB2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLPR2

    Plasmid

    #209980

    Purpose

    Contains Level 0 Part: resistance cassette (cat) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    resistance cassette (cat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLPR3

    Plasmid

    #209986

    Purpose

    Contains Level 0* Part: resistance cassette (ermBL) for the construction of Level 2 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    resistance cassette (ermBL)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLPR1

    Plasmid

    #209979

    Purpose

    Contains Level 0 Part: resistance cassette (ermBL) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    resistance cassette (ermBL)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-SpCas9-P2A-puro

    Plasmid

    #214358

    Purpose

    Expresses SpCas9 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9 and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Cry2(1-531)-mCh-BAXS184E

    Plasmid

    #117238

    Purpose

    Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

    Depositor

    Robert Hughes

    Article

    Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

    Insert

    Cry2531-mCh-BAXS184E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. VGluT1-SNAPtag

    Plasmid

    #107371

    Purpose

    Synaptic vesicle protein VGluT1 fused to the SNAPtag domain

    Depositor

    David Perrais

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    Vesicular Glutamate transporter 1 (Slc17a7 Rat)
    

    Use

    Tags

    SNAPtag, 104 aa before C term

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSW457 - tCLV3

    Plasmid

    #115980

    Purpose

    entry vector for GreenGate cloning method, terminator

    Depositor

    Jan Lohmann

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    tCLV3
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSW455 - pCLV3

    Plasmid

    #115979

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Jan Lohmann

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pCLV3
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pVL26 - pSCR

    Plasmid

    #116021

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pSCR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    T171A by SDM to abolish Eco31I site

    Promoter

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSW614 - pREV

    Plasmid

    #115989

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pREV
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSW618 - pAHP6

    Plasmid

    #115991

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pAHP6
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge