We narrowed to 15,681 results for: LEA

1. pSrpR-BCD20-Cerulean-ssrA P65U23C89T19 v50-2a (587)

   Plasmid

   #70744

   Purpose

   for building 3-color reporter plasmid

   Depositor

   Richard Murray

   Article

   Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

   Insert

   Cerulean-ssrA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5 FRT TO myc Separase delta 55/Non-Cleavable

   Plasmid

   #59827

   Purpose

   Allows the integration of myc Separase delta 55/Non-Cleavable in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

   Insert

   Separase (ESPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   delta 55/Non-Cleavable (E1483R, R1486E, E1503R, R…

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera1

   Plasmid

   #79379

   Purpose

   Cas9 gRNA variant to recruit ms2 proteins

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   Chimera 1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4

   Plasmid

   #79382

   Purpose

   Cas9 gRNA variant to recruit ms2 proteins

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   Chimera 4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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5. hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

   Plasmid

   #24334

   Depositor

   Didier Stainier

   Article

   Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

   Insert

   H2B
   

   Use

   Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Mutation

   mCherry driven by an HSP70 promoterCerulean drive…

   Promoter

   Available Since

   April 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving

   Plasmid

   #204811

   Purpose

   Bacterial expresison of Enterovirus A71 2A protease

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   A71 2A protease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. CA-0252 CMVTO-c-myc-mono-hIL10 (R5A11M)-rens-cleaved

   Plasmid

   #231215

   Purpose

   Cleaved-myc-IL-10

   Depositor

   Xiaojing Gao

   Article

   Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

   Insert

   c-myc-mono-hIL10 (R5A11M)-rens-cleaved (IL10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N36I, N110I, K117N, F129L

   Promoter

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

   Plasmid

   #110201

   Purpose

   Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   LexPR transactivator - 2A - Cerulean
   

   Use

   Tags

   Cerulean

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin

   Plasmid

   #223959

   Purpose

   Dual fluorescent reporter for histone H2B and for APC/C activity

   Depositor

   Hee Won Yang

   Article

   Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

   Insert

   H2B-iRFP670-p2a-mCerulean-Geminin (1–110) (GMNN, H2B Human)
   

   Use

   Lentiviral

   Tags

   iRFP670 and mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin

    Plasmid

    #223965

    Purpose

    Dual fluorescent reporter for histone H2B and for Cdt1

    Depositor

    Hee Won Yang

    Article

    Zhang et al Nat Commun. 2023 Nov 29;14(1):7847. doi:

    Insert

    H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100) (H2B, CDT1 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670 and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SPOV strain SM-6 V-1 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228656

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Spondweni virus for biochemical assay

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Spondweni virus portion of NS2B protease fused to NS3
    

    Use

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. POWV strain LB NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228654

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Powassan virus for biochemical assay

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Powassan virus portion of NS2B protease fused to NS3
    

    Use

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    Alanine mutant to stop self-cleaving ..VEREEA*AL.…

    Promoter

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. WESSV strain SAH177 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228659

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Wesselsbron virus for biochemical assay

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Wesselsbron virus portion of NS2B protease fused to NS3
    

    Use

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. SLEV strain Kern217 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228657

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from St. Louis encephalitis virus for biochemical assay

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    SLEV portion of NS2B protease fused to NS3
    

    Use

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. MVEV strain MVE-1-51 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228653

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Murray Valley encephalitis virus for biochemical assay

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    MVEV portion of NS2B protease fused to NS3
    

    Use

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. DENV-4 strain H241 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228652

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 4 for biochemical assays

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Dengue 4 portion of NS2B protease fused to NS3
    

    Use

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10

    Plasmid

    #104619

    Purpose

    Expresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available Since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(Q63L)-FKBP-mVenus-CAAX

    Plasmid

    #214282

    Purpose

    Express split-RhoA fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
    ras homolog family member A (RHOA Human)
    

    Use

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    RhoA-Q63L

    Promoter

    CMV

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. EV-D68 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

    Plasmid

    #204817

    Purpose

    Bacterial expresison of Enterovirus D68 3C protease

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    EV D68 3C protease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

    Plasmid

    #204816

    Purpose

    Bacterial expresison of Enterovirus A71 3C protease

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    EV A71 3C protease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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