We narrowed to 15,905 results for: grna
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Plasmid#126912PurposePlasmid containing gRNA expression cassette targeting ADE2 in Saccharomyces cerevisiaeDepositorInsertADE2 gRNA
UseCRISPRAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-nCas9n-nanos 3’UTR
Plasmid#62542Purposeexpression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafishDepositorAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJR85
Plasmid#140095PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralExpressionMammalianAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1
Plasmid#138461Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64DepositorPublicationInsertMCP-VP64-IRES-zsGreen1
UseCRISPR and LentiviralExpressionMammalianPromoterEF1AAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting
Plasmid#188541PurposePlasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codonDepositorInsertgRNA
UseCRISPRTagsGFPPromoterU6Available sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601 miniCMV-SaCas9-SpA-sgRNA scaffold
Plasmid#107055PurposeBackbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)DepositorTypeEmpty backboneUseAAV and CRISPRExpressionMammalianAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORANGE unc13a GFP KI
Plasmid#131498PurposeEndogenous tagging of Munc13-1: C-terminal (amino acid position: A1762)DepositorAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
U6-pegRNA-H1-nick sgRNA-mCherry
Plasmid#176901PurposeExpresses prime editing guide RNA and guide RNA in mammalian cellsDepositorInsertsmCherry
gRNA scaffold
gRNA scaffold
UseAAVExpressionMammalianPromoterCAG, H1, and U6Available sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
PLJR962
Plasmid#115162PurposeSth1 dCas9 TetR and KanR L5 Int attP for M. smegmatisDepositorInsertsSth1 sgRNA scaffold
Sth1 dCas9
TetR
L5 attP
L5 Int
KanR
UseUnspecifiedAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PLJR965
Plasmid#115163PurposeSth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosisDepositorInsertsSth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR
UseUnspecifiedAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9(BB)-2A-Neo
Plasmid#127762PurposeUsed for expression of sgRNA in mammalian cellsDepositorTypeEmpty backboneExpressionMammalianAvailable sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP
Plasmid#188777PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORANGE GFP-Grin2a KI
Plasmid#131486PurposeEndogenous tagging of GluN2a: N-terminal (amino acid position: A25)DepositorAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
U6d-T
Plasmid#117212PurposeExpresses gRNA in Aedes aegyptiDepositorInsertu6d promoter-gRNA
UseSynthetic BiologyExpressionInsectPromoterU6Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI
Plasmid#189923PurposeAAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e V106W
UseAAVMutationnSaCas9 KKH, TadA ABE8e V106WPromoterEFSAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330 Mouse 3' Hp1a gRNA
Plasmid#127898PurposeWT Cas9 Vector targeting the 3' end of the mouse Hp1a geneDepositorInsertgRNA/Cas9
UseCRISPRAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330 Mouse 5' Npm1 gRNA
Plasmid#127900PurposeWT Cas9 Vector targeting the 5' end of the mouse Npm1 geneDepositorInsertgRNA/Cas9
UseCRISPRAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry
Plasmid#188766PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterEF1aAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRB1081
Plasmid#71479PurposeExpresses gRNA for dpy-10 conversion using NGAG PAM and VQR Cas9 variantDepositorInsertdpy-10 gRNA
UseCRISPRExpressionWormPromoterU6Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only