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pScalps_puro_mIL-2Rβ

Plasmid

#59916

Purpose

Expresses mouse interleukin-2 receptor Beta chain

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

interleukin 2 receptor beta (Il2rb Mouse)

Use

Lentiviral

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
pIZ-Flag6His-Siwi

Plasmid

#50560

Purpose

Expresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect cells

Depositor

Yukihide Tomari

Article

Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

Insert

Siwi (Aubergine )

Tags

6xHIS and FLAG

Expression

Insect

Promoter

OpIE2

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pMX-Brn4

Plasmid

#47028

Purpose

Expresses mouse Brn4

Depositor

Hans Schöler

Article

Han et al Cell Stem Cell. 2012 Apr 6;10(4):465-72.

Insert

Brn4 (Pou3f4 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

The insert includes 40 bp of the TOPO vector in t…

Available Since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-6P-Cdh1wt (570)

Plasmid

#39877

Depositor

Jonathon Pines

Insert

FZR1 (FZR1 Human)

Tags

GST

Expression

Bacterial

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only
pFbx15(-1172/+114)-Luc

Plasmid

#13412

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pFLAG rat Antizyme 1

Plasmid

#11183

Depositor

Bruce Zetter

Article

Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

Insert

AZ1 (Oaz1 Rat)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 17, 2005

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-1 MKK4(EE)-MKK7a1(EE)

Plasmid

#47580

Purpose

Bacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Inserts

MKK4-EE (MAP2K4 Human)
MKK7a1-EE (Map2k7 Mouse)

Expression

Bacterial

Mutation

Lacks the first 36 residues of the RefSeq protein…

Available Since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only
pEF6-Lck-TurboID

Plasmid

#159433

Purpose

expresses Lck-TurboID in mammalian cells

Depositor

Arthur Salomon

Article

Chua et al J Proteome Res. 2020 Nov 13. doi: 10.102

Inserts

Lck (LCK Human)
TurboID (BirA mutant)

Tags

HA

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

EF-1a

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry GRK2 ct in pcDNA3.1

Plasmid

#64212

Purpose

mCherry fused between Cry2 and GRK2ct to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK2 ct (GRK2 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV and cmv

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only
pET28 His6-mEGFP-D4

Plasmid

#226372

Purpose

Production of recombinant mEGFP-D4, a fluorescent probe binding cholesterol

Depositor

Catherine Tomasetto

Article

Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

Insert

D4 (pfoA Clostridium perfringens)

Tags

His6 and monomeric EGFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLY094-EFS-CD22-Blast-WPRE

Plasmid

#192195

Purpose

Lenti-CD22-OE-Blast

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-CD22-OE-Blast (CD22 Synthetic)

Use

Lentiviral; Mammalian expression

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-eGFP-attP(bxb)-*BsdR

Plasmid

#183751

Purpose

Bxb1 landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

loxP-eGFP-attP-Bsd-lox251

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-EGFP-NLS

Plasmid

#165441

Purpose

Expresses nuclear EGFP from CMV promoter

Depositor

Esteban Engel

Article

Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

Insert

EGFP-NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

Plasmid

#112926

Purpose

CRISPR-a reporter assay lentiviral vector (empty gRNA control)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(empty), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNeonGreen-LEHD-NanoLuc

Plasmid

#98289

Purpose

caspase-9 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-SYP-HRP

Plasmid

#117186

Purpose

Plasmid name in publication: pAAV-DIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

SYP-HRP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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aav-CAG-tdNfsB(F124W)-mCherry (eNTR)

Plasmid

#113761

Purpose

AAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the CAG promoter

Depositor

Luke Lavis

Article

Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

Insert

tdNfsB(F124W)-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

F124W

Promoter

CAG

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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CD4-C-GFP HDRT Source (pTR 152)

Plasmid

#112018

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-GFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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sgPBRM1-2- LentiCRISPRv2

Plasmid

#107404

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEM705 Gsi (Cys)

Plasmid

#109373

Purpose

GsX chimera with Gαi C-terminus (Pertussis toxin sensitive)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsi chimera

Tags

None

Expression

Mammalian

Mutation

Cys at position 391 (Pertussis Toxin Sensitive)

Promoter

CAG

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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