We narrowed to 58,550 results for: tra

1. tetO-MYOCD

   Plasmid

   #170696

   Purpose

   doxycycline-inducible overexpression of MYOCD

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   MYOCD (MYOCD Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AY22_pgRNA.R5.1

   Plasmid

   #100294

   Purpose

   CCR5-targeting gRNA expression plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   gRNA spacer
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   U6 RNA Pol III promoter human snRNA

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-gRNA

   Plasmid

   #158973

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

   Depositor

   Charles P. Lai

   Article

   Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

   Insert

   gRNA3
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV-CAG-oScarlet-WPRE-BC12-pA

   Plasmid

   #220776

   Purpose

   Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

   Depositor

   Vijay Samineni

   Article

   Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

   Insert

   oScarlert-WPRE-BC12
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. amilCP_Orange chromoprotein

   Plasmid

   #117850

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Orange chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amilCP_Orange
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   64V,65G, BioBrick sites removed

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. gfasPurple chromoprotein

   Plasmid

   #117849

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses gfasPurple chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, gfasPurple
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40

   Plasmid

   #100833

   Purpose

   AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

   Promoter

   Synapsin

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Str-STIM1-NN_ST-SBP-EGFP

   Plasmid

   #65262

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. tetO-SMAD6

   Plasmid

   #170700

   Purpose

   doxycycline-inducible overexpression of SMAD6

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   SMAD6 (SMAD6 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. spisPink chromoprotein

    Plasmid

    #117839

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses spisPink chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, spisPink
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lyn-FKBP-FKBP-CFP (LF2C)

    Plasmid

    #20149

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    Lyn-FKBP-FKBP-CFP (FKBP1A Human)
    

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Available Since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.CAG.Flex.GCaMP6s.WPRE.SV40

    Plasmid

    #100842

    Purpose

    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

    Promoter

    CAG

    Available Since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. asPink chromoprotein

    Plasmid

    #117838

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses asPink chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, asPink
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. scOrange chromoprotein

    Plasmid

    #117840

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses scOrange chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, scOrange
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2xFLAGhYAP1

    Plasmid

    #17791

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Tags

    2xFlag

    Expression

    Mammalian

    Available Since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. His-PIMT

    Plasmid

    #34852

    Depositor

    Bruce Downie

    Article

    Dinkins et al Plant J. 2008 Jul;55(1):1-13.

    Insert

    PIMT (PCMT1 Human)
    

    Tags

    6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pmiRGLO Dual-Luciferase-MYB-3'UTR

    Plasmid

    #182250

    Purpose

    Human MYB 3` UTR region containing 3x binding sites for miR-150 cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

    Insert

    3' UTR region of MYB gene
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4/TO-Flag-H3.3

    Plasmid

    #47980

    Purpose

    tetracycline-inducible expression of human Flag tagged H3F3A

    Depositor

    Bing Zhu

    Article

    Huang et al PLoS Genet. 2013 Jun;9(6):e1003558. doi:

    Insert

    H3F3A (H3-3A Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    288 bp T to C synonymous mutation

    Promoter

    CMV promoter with tetracycline operator

    Available Since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pAV-CAG-oScarlet-WPRE-BC6-pA

    Plasmid

    #220770

    Purpose

    Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

    Insert

    oScarlert-WPRE-BC6
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hsyn-flex-dsRed-shvgat

    Plasmid

    #67845

    Purpose

    Expresses shRNA to the vesicular GABA transporter VGAT. Cre dependent expression by flex switch

    Depositor

    William Wisden

    Article

    Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

    Insert

    AAV-hsyn-flexed-dsRed-miR30-vgat2.shRNA
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and RNAi

    Expression

    Mammalian

    Promoter

    h-synapsin

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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