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pEN017-SpCas9-hiNLS-t-M4

Plasmid

#234455

Purpose

Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

Depositor

Ross Wilson

Article

Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

Insert

SpCas9 hiNLS t-M4

Use

CRISPR

Tags

His6, Nucleoplasmin, SV40, and c-Myc

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHIV-IL13-GAPGlySer-PDGFR

Plasmid

#240247

Purpose

Lentiviral transfer plasmid encoding IL13 with GAP-Gly-Ser linker and PDGFR TM

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

IL-13 (IL13 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLv-HSA-uDys/eGFP

Plasmid

#26810

Depositor

Jeffrey Chamberlain

Article

Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

Insert

dystrophin (DMD Human)

Use

Lentiviral; Lentiviral plasmid backbone from l. n…

Tags

eGFP

Mutation

deleted R4-R23 deleted exons 71-78

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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EF1a_POU5F1_P2A_Hygro_Barcode

Plasmid

#120474

Purpose

Barcoded lentiviral vector to express POU5F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

POU5F1 (POU5F1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC1-dmito-oROS-HT

Plasmid

#216418

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

dmito-oROS-HT

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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TH1203-pOCC177_pOEM1-N-HIS-MBP-PS-TAF15_opt-TEV-SNAP

Plasmid

#221891

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

TAF15 (TAF15 Human)

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-GINKO2

Plasmid

#177116

Purpose

Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2

Depositor

Robert Campbell

Article

Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

Insert

GINKO2

Tags

6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB N-term-mNeongreen

Plasmid

#169226

Purpose

Targeting vector backbone to support a knock-in of mNeongreen-Linker at the N-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mNeongreen

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon TVA-mCherry

Plasmid

#131779

Purpose

INTRSECT 2.0

Depositor

Karl Deisseroth

Article

Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

Insert

TVA-mCherry

Use

AAV; Intrsect

Tags

mCherry

Expression

Mammalian

Promoter

nEF

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEN019-SpCas9-hiNLS-t-M1M4

Plasmid

#234456

Purpose

Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

Depositor

Ross Wilson

Article

Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

Insert

SpCas9 hiNLS t-M1M4

Use

CRISPR

Tags

His6, Nucleoplasmin, SV40, and c-Myc

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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prhaBAD-anti-mouse-Nb-Hia5

Plasmid

#239626

Purpose

Rhamnose inducible expression plasmid for anti-mouse nanobody fusion to Hia5 DNA adenine methyltransferase

Depositor

Aaron Straight

Article

Gamarra et al bioRxiv [Preprint]. 2025 Mar 14:2025.03.

Insert

Hia5

Tags

6HIS, MBP, TEV, and anti-Mouse Nanobody

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Promoter

rhaBAD

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF15_P2A_Hygro_Barcode

Plasmid

#120501

Purpose

Barcoded lentiviral vector to express KLF15 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF15 (KLF15 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC1-oROS-HT-CaaX

Plasmid

#216417

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT-CaaX

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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AAV pEF1a-DIO-FLPo-WPRE-hGHpA

Plasmid

#87306

Purpose

An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

Depositor

Li Zhang

Article

Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

Insert

Cre-dependent mouse codon-optimized Flp recombinase.

Use

AAV

Tags

N/A

Expression

Mammalian

Promoter

EF1a

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_FOXA1_P2A_Hygro_Barcode

Plasmid

#120438

Purpose

Barcoded lentiviral vector to express FOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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hnRNPA1-FLAG-IRES-eGFP

Plasmid

#234619

Purpose

Mammalian expression of full-length hnRNPA1 with C-terminal FLAG tag co-expressing with eGFP via IRES sequence

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1 (HNRNPA1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

C-terminal FLAG tag, co-expressing with eGFP via …

Promoter

CMV

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a_FOXA2_P2A_Hygro_Barcode

Plasmid

#120439

Purpose

Barcoded lentiviral vector to express FOXA2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

FOXA2 (FOXA2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term SRSF7

Plasmid

#187582

Purpose

Express FLAG epitope and APEX2-tagged SRSF7 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

SRSF7 (SRSF7 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-dV-IgG1/κ

Plasmid

#52213

Purpose

Human immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

Human immunoglobulin gamma 1 constant region
Human immunoglobulin kappa constant region

Expression

Mammalian

Mutation

Deleted Variable region

Promoter

Mouse Elongation Factor 1 Alpha and Rat Elongatio…

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-173-N-ORAI1

Plasmid

#87618

Purpose

N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

Venus173-N-ORAI1 (ORAI1 Human, Synthetic)

Tags

6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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