We narrowed to 10,006 results for: Gnas

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pPBbleo-EGF-ScNeo

Plasmid

#209902

Purpose

To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

EGF-ScNeo (EGF )

Tags

GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pHOT-P2Y2R

Plasmid

#159108

Purpose

entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the Gibson

Depositor

Fabio Mammano

Article

Mazzarda et al Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc

Insert

P2Y2R

Use

Lentiviral

Promoter

UBC

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB WT

Plasmid

#60419

Purpose

Mammalian Expression of ITSN DH/PH-tgRFPt-Nano

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

hITSN1-tgRFPt-SSPB (ITSN1 Human, Synthetic)

Use

Cre/Lox and Lentiviral

Tags

tgRFPt

Expression

Mammalian

Mutation

hITSN 1159-1509 // SSPB Y11K and A15E

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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FERMT1_pLX307

Plasmid

#98333

Purpose

Lentiviral expression of FERMT1

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

FERMT1 (FERMT1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-p57

Plasmid

#101760

Purpose

p57 promoter reporter

Depositor

Yuh-Shan Jou

Article

Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

Insert

cyclin dependent kinase inhibitor 1C promoter (CDKN1C Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mScarlet-CAAX2 WPRE

Plasmid

#236232

Purpose

AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mScarletI fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mScarletI

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-NR4A3-P2A-mCherry

Plasmid

#203863

Purpose

Tet-inducible lentiviral plasmid expressing NR4A3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

NR4A3 (NR4A3 Human)

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-NR4A1-P2A-mCherry

Plasmid

#203859

Purpose

Tet-inducible lentiviral plasmid expressing NR4A1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

NR4A1 (NR4A1 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-hPKL WT

Plasmid

#107159

Purpose

For mammalian expression of Flag-hPKL WT

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

Flag-hPKL WT (PKLR Human)

Use

Retroviral

Tags

N-term Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-α-Syn-nYFP

Plasmid

#92203

Purpose

Retroviral overexpression vector for α-Syn bimolecular fluorescence complementation (BiFC)

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

SNCA (SNCA Human)

Use

Retroviral

Tags

YFP(1-155)

Expression

Mammalian

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3xFLAG-RSL1D1(WT)

Plasmid

#122301

Purpose

expresses RSL1D1 protein with a flag tag

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RSL1D1 (RSL1D1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/FGFR2-S252W(Apert) myc

Plasmid

#33249

Depositor

Claudio Basilico, Alka Mansukhani

Article

Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-30

Insert

FGFR2 (Fgfr2 Mouse)

Tags

His and Myc

Expression

Mammalian

Mutation

Mutation S252W in FGFR2 found in Apert syndrome. …

Promoter

CMV

Available Since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only
pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)

Plasmid

#201819

Purpose

A plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoter

Depositor

Christopher Richie

Article

Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

Insert

hChR2(H134R)-V5-ER-LBD (ChRERa)

Expression

Mammalian

Promoter

pOTTC1751

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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BMPR1A_pLX307

Plasmid

#98320

Purpose

Lentiviral expression of BMPR1A

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

BMPR1A (BMPR1A Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSems-leader-mEGFP-MBP-TMD(ALA7)

Plasmid

#193051

Purpose

Expression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

ALA7-TMD

Tags

Ig k-chain leader sequence, MBP, and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi-FoxO1_1R_13A_3D

Plasmid

#106279

Purpose

Fluorescent fusion protein for FoxO1

Depositor

Laura Heiser

Article

Gross et al Cell Syst. 2019 Dec 18;9(6):580-588.e4.

Inserts

Foxo1a (Foxo1 Mouse)
NLS-mCherry-NLS-P2A-Puromycin

Tags

Clover fluorescent protein and NLS

Expression

Mammalian

Mutation

Deleted amino acids 401-636, T24A, S209A, H212R, …

Promoter

EF1a/RPBSA

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pUAS-ERK-YFP

Plasmid

#42076

Depositor

Norbert Perrimon

Article

Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

Insert

Drosophila ERK ortholog (rl Fly)

Tags

YFP

Expression

Insect

Promoter

Hsp70

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
retro-gfp-puro vector

Plasmid

#58249

Purpose

Retroviral expression vector encoding bicistronic EGFP and Puromycin cassette

Depositor

Georgios Stathopoulos

Article

Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

Insert

EGFP

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB R73Q

Plasmid

#60420

Purpose

Mammalian Expression of ITSN DH/PH-tgRFPt-Micro

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

hITSN1-tgRFPt-SSPB (ITSN1 Human, Synthetic)

Use

Cre/Lox and Lentiviral

Tags

tgRFPt

Expression

Mammalian

Mutation

hITSN 1159-1509 // SSPB R73Q, Y11K and A15E

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pSH1/M-FGFR2-Fv-Fvls-E

Plasmid

#15286

Depositor

David Spencer

Article

Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

Insert

FGFR2 kinase, FKBP12v36 (Fgfr2 Mouse)

Tags

Myristoylation-targeting domain c-Src (14aa) and …

Expression

Mammalian

Mutation

FGFR2 cytoplasmic domain FKBP12 F36V

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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