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pPRIME-CMV-GFP-shNts2

Plasmid

#132714

Purpose

Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Nts

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shNts(2) (Nts Rat)

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLX302 GDF11-V5 puro

Plasmid

#83097

Purpose

Lentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

GDF11 (GDF11 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pCMMP-BiP-IRES-mRFP

Plasmid

#36975

Depositor

Bill Sugden

Article

Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

Insert

BiP (HSPA5 Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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LTN1_pCSdest

Plasmid

#53855

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

LTN1 (LTN1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO.1 shSV40 neo

Plasmid

#158646

Purpose

Lentiviral shRNA vector targeting simian virus 40 T antigen (SV40Tag)

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

SV40Tag (SV40gp6 Simian virus 40)

Use

Lentiviral

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK Tgfbr3-HA neo

Plasmid

#58508

Purpose

Lentiviral expression vector for an inducible HA-tagged mouse Tgfbr3

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

Tgfbr3_HA (Tgfbr3 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only
pLentiPGK Puro DEST JNKKTRmCerulean3

Plasmid

#90232

Purpose

Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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TMPRSS3_pCSdest

Plasmid

#53839

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

TMPRSS3 (TMPRSS3 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)

Plasmid

#85041

Purpose

This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

loxP-stop-loxP

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-C9orf72

Plasmid

#83439

Purpose

Expresses Cas9 and a gRNA targeting C9orf72

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

gRNA targeting human C9orf72 (C9orf72 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-miR-9 sponge

Plasmid

#25040

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

miR-9 sponge

Use

Retroviral

Tags

d2EGFP

Expression

Mammalian

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA(lib)-puro

Plasmid

#119976

Purpose

Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Wensheng Wei

Article

Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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sgPBRM1-1- LentiCRISPRv2

Plasmid

#107403

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCMMP-2xmyc-Bcl2A1-IRES-mRFP

Plasmid

#36973

Depositor

Bill Sugden

Article

Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

Insert

BCL2A1 (BCL2A1 Human)

Use

Retroviral

Tags

2xMYC

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only
sgPBRM1-2- LentiCRISPRv2

Plasmid

#107404

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

Plasmid

#129668

Purpose

A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Tags

GFP

Expression

Mammalian

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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MAC_N_CDK8

Plasmid

#111685

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CDK8 (CDK8 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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shPBRM1#32-Tet-pLKO-puro

Plasmid

#107399

Purpose

Lentiviral expression of shRNA targeting PBRM

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

shRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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shPBRM1#131-Tet-pLKO-puro

Plasmid

#107400

Purpose

Lentiviral expression of shRNA targeting PBRM

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

shRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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MDH1-PGK-FLAG-TAT-86-E

Plasmid

#35978

Depositor

Susana Valente

Article

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

transactivating protein (tat Human Immunodeficiency Virus)

Use

RNAi and Retroviral

Tags

Flag

Expression

Mammalian

Mutation

From the "E" HIV clade

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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