170,224 results
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Plasmid#199248PurposeFluorescent labelling of bacterial cells with sfGFPDepositorInsertCodon-optimized sfGFP gene
ExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Hygro
Plasmid#127763PurposeUsed for expression of sgRNA in mammalian cellsDepositorTypeEmpty backboneExpressionMammalianAvailable SinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET47b-T7-enAsCas12f
Plasmid#204642PurposeExpression of enAsCas12f in E. coliDepositorInsertenAsCas12f
Tags6xHisExpressionBacterialMutationD196K, N199K, G276R, N328G, D364RPromoterT7Available SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-GSK3β-#1
Plasmid#32496DepositorAvailable SinceSept. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHex
Plasmid#124670PurposeExpresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coilDepositorInsertSpyCatcher002-oHex
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spCas9-ABE-Umax-rest1
Plasmid#222144PurposeAdenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9
UseCRISPR; Zebrafish expressionTagsBipartite NLSPromoterT3Available SinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tol2
Plasmid#31823DepositorInsertTol2 Transposase
ExpressionMammalianPromoterCMVAvailable SinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
hCXCR4-mTQ2
Plasmid#110196PurposeEncodes for human CXCR4 coding sequence tagged with the mTQ2 FPDepositorAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VEGFR1-V5/HIS
Plasmid#205616PurposeExpression of human VEGFR1 receptor tyrosine kinase in mammalian cellsDepositorAvailable SinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
TfR-sfGFP-myc tag-SpyCatcher003
Plasmid#133451PurposeExpresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detectionDepositorInsertTfR-sfGFP-myc tag-SpyCatcher003
TagsGSSGS and myc tagExpressionMammalianMutationContains C20 and A23 mutations that improve plasm…PromoterCMVAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2
Plasmid#174665PurposeExpresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoterDepositorInsertsLUC2
mCherry:T2A:Hygro
UseLentiviral and LuciferaseTagsmCherry and hygromycin dual reporter gene linked …ExpressionMammalianPromoterHuman cytomegalovirus immediate early enhancer/pr…Available SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1
Plasmid#138460Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRABDepositorInsertMCP-KRAB-IRES-zsGreen1
UseCRISPR and LentiviralExpressionMammalianPromoterEF1AAvailable SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsUpf1-siRNAres_P
Plasmid#147196PurposeMammalian Expression of HsUpf1-siRNAresDepositorInsertHsUpf1-siRNAres (UPF1 Human)
ExpressionMammalianMutation5 non silent mutations A69S, E167G, K282R, A442G…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBC-SV40NLS-Pfv-Sapphire-PURO
Plasmid#203654PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.DepositorArticleInsertPfV
UseLentiviralTagsSapphireAvailable SinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK435
Plasmid#236573PurposeCloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into TRV2 Architecture_BDepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
Pyronic/pcDNA3.1(-)
Plasmid#51308PurposePyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.DepositorInsertPyronic
ExpressionMammalianPromoterCMVAvailable SinceFeb. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast
Plasmid#223173PurposeExpresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.DepositorInserthfCas13d (N2V8)
UseLentiviralTagsNLS-HAAvailable SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330S-2-PITCh
Plasmid#63670PurposeExpresses Cas9 nuclease and the PITCh-gRNADepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Blast empty (w263-1)
Plasmid#17486Purpose3rd gen lentiviral empty control vector, CMV promoter, BlastDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
TFORF0988
Plasmid#143455PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only