We narrowed to 12,331 results for: NSI
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Plasmid#124939PurposeExpresion of RGG_GFP-RGGDepositorInsertRGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
TagsGFPExpressionMammalianMutationTEV site between RGG and EGFPAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
tdp43-EGFP construct8
Plasmid#28201DepositorInsertTar DNA binding protein (TARDBP Human)
TagsEGFPExpressionMammalianMutationaa275-313 deletedAvailable SinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_eCB2.0mut
Plasmid#164607PurposeExpress the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neuronsDepositorInsertEndocannabinoid non-responsive sensor GRAB_eCBmut
UseAAVMutationS383T, F177APromoterhSynAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP-P2A-FusionRed
Plasmid#191108PurposeMammalian cell line expression plasmids for production of EGFP and FusionRed proteinsDepositorInsertpAAV-CAG-EGFP-P2A-FusionRed
UseAAVTagsFusionRedExpressionMammalianMutationSee depositor comments belowPromoterchicken β-actin promoterAvailable SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-STK4
Plasmid#172989Purposeconstitutive expression of FLAG-tagged STK4DepositorAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pMXs-IG
Plasmid#51258PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable SinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FIH1
Plasmid#21399DepositorInsertHIF1AN (HIF1AN Human)
ExpressionMammalianAvailable SinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OK+9MS-Cas9
Plasmid#120354PurposepiggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.DepositorInsertOK+9MS cassette
UseCRISPR; Piggybac transposonExpressionMammalianMutationKlf4 [1-483]Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP
Plasmid#232754PurposeExpresses EGFPDepositorInsertEGFP
UseLentiviralPromoterEF1aAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7REwt
Plasmid#213935PurposeGST tagged AO7 encoding aa 126-258 for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationAO7 cloned into the first multiple cloning site o…PromoterT7 promoter-1Available SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQdCas9.luxR(mut)-sggfp
Plasmid#236185PurposeThe plasmid pQdCas9.luxR(mut)-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the GFP gene. Additionally, this plasmid contains a mutation in the luxR gene.DepositorInsertQuorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
PromoterQuorum sensing promoterAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQdCas12a.luxR(mut)-sggfp(C)
Plasmid#236043PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
UseCRISPRPromoterquorum sensing promoterAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQdCas12a.luxR(mut)-empty
Plasmid#236037PurposeThe plasmid pQdCas12a.luxR(mut)-empty expresses the dCas12a endonuclease. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI, dcas12a, and lacZ-alpha (fragment).
UseCRISPRPromoterquorum sensing promoterAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TXK-1/1
Plasmid#91539PurposeProtein expression and purification of human SH3 domain construct TXK-1/1DepositorInsertTXK-1/1 (TXK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET_SZ1-RGG-GFP-RGG
Plasmid#124937PurposeExpression of SZ1-RGG-GFP-RGGDepositorAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
HR-TERT-promotor
Plasmid#71398PurposeHomologues recombination donor plasmid fo the wild type TERT promotor region. Includes 1kb of TERT coding region in right homologues arm.DepositorInsertTERT (TERT Human)
PromoterEndogenous TERT promotorAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
U6-CjCas9-MS2gRNA-Backbone
Plasmid#210711PurposeThis plasmid express MS2 loop containing Cj Cas9 gRNADepositorInsertMS2 loop containing Cj Cas9 gRNA
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2 EGFP sgRNA2
Plasmid#164933PurposeExpresses EGFP sgRNA2 in mammalian cellsDepositorInsertsgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)
UseLentiviralPromoterEFS promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG32-3HA-3FLAG
Plasmid#154081PurposeFor C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.DepositorInsert3xHA-3xFLAG-hygR
UsePcr-based tagging in yeastTags3xHA-3xFLAGAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA
Plasmid#159283PurposeA multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).DepositorInsertsHuman INS sgRNA
Human INS guide RNA
ExpressionMammalianPromoterU6Available SinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only