We narrowed to 14,498 results for: SHR;

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pSIN-Arkadia-gRNA1

Plasmid

#180374

Purpose

targeting mouse Arkadia gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia targeting gRNA (Rnf111 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

human U6

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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p5078 pcDNA4c hBrd4 (1-470)

Plasmid

#14454

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1-470) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1-470

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
LL - hOCT4i -2

Plasmid

#12197

Depositor

George Daley

Article

Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

Insert

OCT4 - RNAi (POU5F1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Sept. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pRDB_118

Plasmid

#216089

Purpose

Cas9 [Sp] CRISPRa targeting CD47, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD47 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuide-RRBP1-1

Plasmid

#92156

Purpose

CRISPR guide RNA targeting human RRBP1

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 sgRNA-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMGIB-3xFLAG-reptin

Plasmid

#53609

Purpose

Retroviral expression of human reptin

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

reptin (RUVBL2 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

CDS mutated to confer resistance to shRNA. All mu…

Promoter

LTR

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pRDA_833

Plasmid

#216086

Purpose

Cas9 [Sp] knockout targeting CD47, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD47 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_INT[3xPP7_SL]

Plasmid

#68424

Purpose

Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct bearing three PP7 Stem-loops

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9

Plasmid

#118842

Purpose

Targets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

cdkn2a gRNA (LOC100329528 Zebrafish)

Use

CRISPR; Tol2

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

Plasmid

#128755

Purpose

Expresses CYRANO gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

CYRANO sgRNA (OIP5-AS1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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Pzac2.1 U6-Fermt2 sgRNA1, 2, 3_gfaABC1D mcherry SV40

Plasmid

#179117

Purpose

Expresses Fermt2 sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

Depositor

Baljit Khakh

Article

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Fermt2 sgRNA

Use

AAV

Promoter

U6

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-3xGFP11-mem-3xControlgRNA

Plasmid

#224583

Purpose

Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

3xGFP11

Use

CRISPR

Tags

Membrane Localization Signal

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-UGCG-KO

Plasmid

#80010

Purpose

gRNA to knock out expression of UGCG gene. The product of this gene catalyzes the first step in synthesis of glycosphingolipids.

Depositor

Sriram Neelamegham

Article

Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

Insert

UGCG (UGCG Human)

Use

CRISPR

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pSaCas9-sgRNA-Tetra-com vector

Plasmid

#131227

Purpose

For expressing gRNA, whose Tetraloop is replaced with a com aptamer

Depositor

Baisong Lu

Article

Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

Insert

vector for com modified sgRNA

Use

AAV

Expression

Mammalian

Available Since

Oct. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS468-pLK.O1-NmeSgRNA/DTS13-Telomere

Plasmid

#85714

Purpose

Telomere-targeting Nme sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

telomere sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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p8103 LentiCRISPR v2 sgNT-2

Plasmid

#163313

Purpose

Expresses Cas9 and a non-targeting control guide RNA

Depositor

Elizabeth White

Article

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

sgNT-2

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pVG1

Plasmid

#111444

Purpose

Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgScADE2/stop-codon repair

Use

CRISPR

Expression

Yeast

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pBB75-ncRNA

Plasmid

#194087

Purpose

Expresses Ec86 ncRNA in Escherichia coli

Depositor

Tingting Zou

Article

Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

Insert

Ec86 ncRNA

Promoter

T7

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgLKB1 clone3

Plasmid

#162127

Purpose

Lentiviral sgRNA plasmid targeting human LKB1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgLKB1 (STK11 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSMP-RING1_2

Plasmid

#36356

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

RING1 (RING1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only