We narrowed to 10,134 results for: ada

1. pLentiCrisprV2 sgRNA hPER2c-term #1

   Plasmid

   #179454

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCrisprV2 sgRNA hPER2c-term #2

   Plasmid

   #179455

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCrisprV2 sgRNA hPER2c-term #3

   Plasmid

   #179456

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKK-mRuby3-TEV

   Plasmid

   #105780

   Purpose

   Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   mRuby3-TEV

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-GRAB_CRFmut

   Plasmid

   #208659

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Mouse Alpha2 delta-3 pMT2

   Plasmid

   #58727

   Purpose

   expression of mouse Alpha2 delta-3 in mammalian cells

   Depositor

   Annette Dolphin

   Article

   Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

   Insert

   alpha2 delta-3 (Cacna2d3 Mouse)
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKK-TEV-MBP

   Plasmid

   #105787

   Purpose

   Expression of your protein of interest in fusion with MBP at the C-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-MBP

   Expression

   Mammalian

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. AT4G23740 J11_pECIA2

   Plasmid

   #114998

   Purpose

   Bait vector AT4G23740 J11_pECIA2 should be used with prey vector AT4G23740 J11_pECIA14.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT4G23740 (AT4G23740 Mustard Weed)
   

   Tags

   Fc, V5 and hexahistidine tags

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKK-TEV-mRuby3

   Plasmid

   #105797

   Purpose

   Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mRuby3

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p-mCherry-C1-Phobos_CA

    Plasmid

    #98169

    Purpose

    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Phobos_C128A gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-TetOn-ND-Cdt1-mCherry

    Plasmid

    #193761

    Purpose

    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    ND-Cdt1-mCherry (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Mutation

    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

    Promoter

    TRE

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTwist-SARS-CoV-2 Spike-deltaC18 HK.3

    Plasmid

    #212572

    Purpose

    Encodes SARS-CoV-2 spike for HK.3 pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike HK.3 (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE2-TetOminiCMV-OS

    Plasmid

    #136576

    Purpose

    Dox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-P2A-Sox2 (Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5FRT/TO-Ataxin3-Strep-HA

    Plasmid

    #113496

    Purpose

    Expression of human Ataxin-3 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    Ataxin-3 (ATXN3 Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-mDrc7-FLAG

    Plasmid

    #176470

    Purpose

    Expression vector of mouse dynein regulatory complex subunit 7 (Drc7) tagged with FLAG at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

    Insert

    dynein regulatory complex subunit 7 (Drc7 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDB-hCRY1-mClover3-CD4-bla

    Plasmid

    #179441

    Purpose

    Donor vector to knock in mClover3 C-terminal to human CRY1 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    mClover3
    

    Use

    CRISPR

    Tags

    His/Flag

    Promoter

    None

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti CMV-MRLC1-mRuby2-IRES-PuroR

    Plasmid

    #103031

    Purpose

    Lentiviral expression of mRuby2-tagged MRLC with puromycin selection

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    MRLC1 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    mRuby2

    Promoter

    CMV immediate early

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. qTAG-N-Blast-ultraID-LMNB1

    Plasmid

    #207775

    Purpose

    Donor template for Blast-2A-ultraID insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Blast-ultraID Cassette (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pAAV-EFS-DIO-GRAB_CRFmut

    Plasmid

    #208663

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Flag-RNF168 delta RING

    Plasmid

    #133982

    Purpose

    mammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of RING

    Promoter

    CMV

    Available Since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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