We narrowed to 13,292 results for: cDNA

1. pcDNA3.1 myc-mβTrCP WD1-7 (241-569)

   Plasmid

   #62977

   Purpose

   Expresses myc-tagged mouse βTrCP residues 241-569

   Depositor

   Michael Ruppert

   Article

   Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

   Insert

   BTRC (Btrc Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   codes for residues 241-569

   Promoter

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

2. YFP(159-238)-gamma-1 in pcDNAI/Amp

   Plasmid

   #54471

   Purpose

   A carboxyl-terminal YFP fragment was fused to Ggamma-1. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

   Insert

   YFP(159-238)-gamma-1 (GNGT1 Aequorea victoria, Bovine)
   

   Use

   Tags

   YFP(159-238) was fused to the amino terminus of G…

   Expression

   Mammalian

   Mutation

   Ggamma-1 was amplified by PCR, which added a Bam…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 PARL-FLAG-CT S65D+T69D+S70D

   Plasmid

   #13617

   Depositor

   Luca Pellegrini

   Article

   Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

   Insert

   Presenilin-associated rhomboid-like (PARL, PARL Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 65, Thr 69 and Ser70 to Asp

   Promoter

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA5FRT/Flag-hINO80 ΔN ΔHSA (520-1556)

   Plasmid

   #29446

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

   Insert

   INO80 (INO80 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   INO80 520-1556 (a.a.)

   Promoter

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-Lyn-Venus-cpVenus-FLARE-AKAR

   Plasmid

   #123337

   Purpose

   Plasma membrane-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Lyn-Venus-cpVenus-FLARE-AKAR
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase, Ve…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA4TO-3XFLAG Halo delta 1-117 nsp1

   Plasmid

   #175423

   Purpose

   Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 delta 1-117 nsp1
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   nsp1 C-terminus

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935)

   Plasmid

   #49065

   Purpose

   Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   2xHA epitope in ECL4 inserted at aa574 in hNKCC1 …

   Promoter

   CMV

   Available Since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. (366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)

   Plasmid

   #168189

   Purpose

   HA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutation

   Depositor

   Christina Woo

   Article

   Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

   Insert

   HA-nUbc6E-(EAAAK)4-OGT(4,K852A)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA Flag-RARalpha (T181A,S445A,S461A)

   Plasmid

   #35556

   Depositor

   Harish Srinivas, Jonathan Kurie

   Article

   Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

   Insert

   RARalpha (RARA Human)
   

   Use

   Tags

   Flag and His

   Expression

   Mammalian

   Mutation

   T181A, S445A, S461A

   Promoter

   Available Since

   April 5, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA 3.1 LIC 6A NLRP1-FLAG E1322R

    Plasmid

    #166809

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E1322R

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.2-V5-DEST-hADAM10(aa1-213)-GST

    Plasmid

    #189562

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    contains aa 1-213

    Promoter

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S S730A

    Plasmid

    #166801

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    S730A

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5FRT/FLAG-Ino80 ΔNC (267-1261)

    Plasmid

    #29439

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

    Insert

    INO80 (INO80 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    INO80 267-1261 (a.a.) with Q1116K mutation

    Promoter

    Available Since

    Aug. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3 PARL-FLAG-CT S65A+T69A+S70A

    Plasmid

    #13616

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Ser 65, Thr 69 and Ser 70 to Alanine

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA4TO-3XFLAG Halo R124A/K125A nsp1

    Plasmid

    #175417

    Purpose

    Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 R124A/K125A nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5FRT/FLAG-Ino80 ΔNC-EQ (267-1261)

    Plasmid

    #29437

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

    Insert

    INO80 (INO80 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    INO80 267-1261 (a.a.) E653Q

    Promoter

    Available Since

    July 1, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.2 mir-1-1 reporter hsa-mir-128-1

    Plasmid

    #46675

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-128-1 (MIR128-1 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. PcDNA-DEST53-CypB-Golgi N-term GFP

    Plasmid

    #36127

    Depositor

    Patrick Van Oostveldt

    Article

    Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

    Insert

    cyclophilin B (PPIB Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains amino acids 1-211 of cyclophilin B

    Promoter

    CMV

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-1047

    Plasmid

    #69903

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.2 mir-1-1 reporter hsa-mir-92a-1

    Plasmid

    #46672

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-92a-1 (MIR92A1 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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