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pSBtet-Neo

Plasmid

#60509

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and neomycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

TCE & RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pST-KT

Plasmid

#44561

Purpose

for ectopic inducible expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Promoter

UV15 promoter

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)

Plasmid

#126580

Purpose

A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Tags

HA

Expression

Mammalian

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDonor_sU6

Plasmid

#69351

Purpose

sgRNA scaffold and synthetic U6 promoter

Depositor

Andrea Ventura

Article

Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

Insert

sU6 promoter and sgRNA scaffold flanked by BbsI sites

Use

CRISPR

Promoter

synthetic U6 promoter

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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GFP Snail WT

Plasmid

#16225

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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RelB cFlag pcDNA3

Plasmid

#20017

Depositor

Stephen Smale

Insert

RelB (Relb Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau AP P301L

Plasmid

#46906

Purpose

expresses EGFP tagged Tau AP P301L in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau AP P301L (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

P301L AND all 14 S/P or T/P amino acid residues (…

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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STAT3(Y705F)-TAL-Luc

Plasmid

#46933

Purpose

Luciferase Reporter for mutant STAT3

Depositor

Afshin Dowlati

Article

Dabir et al Mol Cancer Res. 2009 Nov;7(11):1854-60.

Insert

STAT3(Y705F) (STAT3 Human)

Use

Luciferase

Tags

FLAG

Mutation

Tyrosine 705 to Phenylalanine

Promoter

TA Luciferase

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only
Human LFA-1 alpha

Plasmid

#8630

Depositor

Timothy Springer

Article

Larson et al J Cell Biol 1989 Feb;108(2):703-12.

Insert

LFA1 alpha (ITGAL Human)

Expression

Bacterial

Available Since

June 23, 2005

Availability

Academic Institutions and Nonprofits only
pX459V2.0-eSpCas9(1.1)

Plasmid

#108292

Purpose

pX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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AICSDP-36: AAVS1-mEGFP (PGK)

Plasmid

#114404

Purpose

Homology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP

Depositor

Allen Institute for Cell Science

Insert

AAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

Sept. 4, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pRS316-RGR-GFP

Plasmid

#51056

Purpose

Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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CIB1-CreC(N1)

Plasmid

#75367

Purpose

Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Cre DNA recombinase, residues 106-343

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pWay21-MKP-1 FL

Plasmid

#13469

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1 FL (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
c-myc promoter (Del 4)

Plasmid

#16604

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Del 4) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Del-4 (PvuII->PvuII). The first PvuII site is …

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only

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pET30-R5-GFP

Plasmid

#17838

Depositor

Jay Keasling

Article

Shaikh et al Anal Chem. 2008 Feb 1. 80(3):886-90.

Insert

pET30-GFP

Use

Synthetic Biology

Tags

His, R5, and S-tag

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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pCAX APP delta CT

Plasmid

#30143

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein (APP Human)

Expression

Mammalian

Mutation

deletion of C-terminal intracellular domain (afte…

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Plasmid

#58442

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 4 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3-STAT3-S727D

Plasmid

#73364

Purpose

Expression of STAT3-S727D

Depositor

Jie Chen

Article

Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

Insert

Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)

Tags

FLAG tagged

Expression

Mammalian

Mutation

E16K S727D

Promoter

CMV

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pEF1-alpha-IIb

Plasmid

#27288

Depositor

Timothy Springer

Article

Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

Insert

integrin alpha IIb (ITGA2B Human)

Tags

His and V5

Expression

Mammalian

Mutation

n/a

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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