We narrowed to 21,668 results for: Tes

1. pOPINK-OTUD3 (OTU+UBA, aa 52-275)

   Plasmid

   #61411

   Purpose

   Expresses human OTUD3 (OTU+UBA domains) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD3 (OTUD3 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-51 and aa 276-398.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. actc1b-mKate2-CDC42-DN

   Plasmid

   #109532

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to dominant negative CDC42

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   CDC42 (cdc42 Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Expression

   Mutation

   DN

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSHUT4-eYFP

   Plasmid

   #133094

   Purpose

   Agrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHI

   Depositor

   Jens Laurids Sørensen

   Article

   Nielsen et al Fungal Biol Biotechnol. 2019 Dec 11;6:25

   Insert

   enhanced yellow flourescent protein
   

   Use

   Fungal expression

   Tags

   Expression

   Mutation

   Promoter

   TEF-1α

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTet-O-KLF7-T2A-PuroR

   Plasmid

   #162346

   Purpose

   Lentiviral expression of KLF7 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   KLF7 (KLF7 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. GA-DEVD-GB-NES

   Plasmid

   #61020

   Purpose

   green ddFP based caspase-3 biosensor

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddGFP A and ddGFP B
   

   Use

   Tags

   HindIII and NES sequence LALKLAGLDIGS placed afte…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCS2plus-mVenus1-155-I152L-GGGS

   Plasmid

   #162613

   Purpose

   Allows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assays

   Depositor

   Marco Morsch

   Article

   Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

   Insert

   mVenus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1-155-I152L

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTet-O-NEUROG3-T2A-PuroR

   Plasmid

   #162341

   Purpose

   Lentiviral expression of NEUROG3 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   NEUROG3 (NEUROG3 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOPINK-Cezanne2 (OTU+UBA, aa 1-462)

   Plasmid

   #61582

   Purpose

   Expresses human Cezanne2 (OTU+UBA domains) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   Cezanne2 (OTUD7A Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Isoform 2. Deleted aa 463-933.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-Flag-LmoCas6

   Plasmid

   #126485

   Purpose

   Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas6
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-Flag-LmoCas8b2

    Plasmid

    #126482

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas8b2
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-Flag-LmoCas7

    Plasmid

    #126483

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas7
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-Flag-LmoCas5

    Plasmid

    #126484

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas5
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA 3.1 AnxA6

    Plasmid

    #20407

    Depositor

    Carlos Enrich

    Article

    Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

    Insert

    AnxA6 (Anxa6 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pOPINK-VCPIP (OTU, aa 25-561)

    Plasmid

    #61583

    Purpose

    Expresses human VCPIP (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    VCPIP (VCPIP1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-24 and aa 562-1222.

    Promoter

    T7

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p3E-CDC42-DN

    Plasmid

    #109584

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative CDC42

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    CDC42 (cdc42 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    dominant negative

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AKT-APEX2-P2A-mKate2

    Plasmid

    #67654

    Purpose

    Mammalian expression vector driving AKT-APEX2 (Human AKT-PH domain, [marks PI(3,4)P2 and PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    AKT-PH-APEX2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. actc1b-mKate2-SERCA1

    Plasmid

    #109498

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    SERCA1 (atp2a1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Please see Depositor Comments

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOPINK-OTUD1 (OTU, aa 287-437)

    Plasmid

    #61407

    Purpose

    Expresses human OTUD1 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD1 (OTUD1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-286 and aa 438-481.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOPINK-OTUB2 (full-length, aa 1-234)

    Plasmid

    #61421

    Purpose

    Expresses human OTUB2 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUB2 (Ubiquitin thioesterase OTUB2) (OTUB2 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3E-DrCav1

    Plasmid

    #194290

    Purpose

    Multisite gateway entry clone for expression of codon optimised zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab clone KRT

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    caveolin1 (cav1.S Frog)
    

    Use

    Multisite gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-LmoCas8b2

    Plasmid

    #126488

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas8b2 driven by hUbC promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas8b2
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti-LmoCas7

    Plasmid

    #126489

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas7 driven by hUbC promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas7
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-LmoCas5

    Plasmid

    #126490

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas5 driven by hUbC promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas5
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mKate2-P2A-APEX2-2xML1N(7Q)

    Plasmid

    #67666

    Purpose

    Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-ML1Nmut*2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-LmoCas6

    Plasmid

    #126491

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6 driven by hUbC promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas6
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pOPINK-OTUD6A (OTU, aa 128-288)

    Plasmid

    #61417

    Purpose

    Expresses human OTUD6A (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6A (OTUD6A Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-127.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. actc1b-mKate2-EEA1

    Plasmid

    #109508

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to early endosome associated 1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    EEA1 (eea1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOPINK-OTUD6B (OTU, aa 133-293)

    Plasmid

    #61419

    Purpose

    Expresses human OTUD6B (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6B (OTUD6B Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-132.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GFP-Miro1 S156E

    Plasmid

    #127589

    Purpose

    Phospho-mimetic of S156

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Miro1 (RHOT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S156E

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA-Flag-EcoCas6

    Plasmid

    #106274

    Purpose

    Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasE
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge