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pGL2-GAL4-UAS-Luc

Plasmid

#33020

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

6X GAL4-UAS

Tags

Luciferase

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-Puro

Plasmid

#141369

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp4 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-Puro

Plasmid

#141392

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf9b

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Str-Ii_SBP-EGFP-Golgin84

Plasmid

#65303

Purpose

synchronize trafficking of Golgin-84 from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Golgin-84 (GOLGA5 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 CB1

Plasmid

#13391

Purpose

Expresses mouse cannabinoid receptor 1 (CB1) in mammalian cells

Depositor

Mary Abood

Article

Abood et al Biochem Pharmacol. 1997 Jan 24. 53(2):20

Insert

CB1 (Cnr1 Mouse)

Expression

Mammalian

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pSico PGK puro

Plasmid

#11586

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes the puromycin gene to be recombined out of the construct, activating shRNA expression.

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pCT302

Plasmid

#41845

Depositor

Dane Wittrup

Article

Midelfort et al J Mol Biol. 2004 Oct 22;343(3):685-701.

Insert

4-4-20

Tags

HA and c-myc

Expression

Yeast

Available Since

Jan. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG2LMKOSimO

Plasmid

#20866

Depositor

Keisuke Kaji

Article

Kaji et al Nature. 2009 Apr 9;458(7239):771-5. doi:

Insert

c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2-ires-mOrange (Myc Mouse)

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPA1A

Plasmid

#19483

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA1A (HSPA1A Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pOPINJ

Plasmid

#26045

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

HisGST3C-lacZ

Tags

MAHHHHHH_GST_LEVLFQGT

Expression

Bacterial, Insect, and Mamm…

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-XRCC4

Plasmid

#46959

Purpose

Plasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

XRCC4 (XRCC4 Human)

Tags

GFP-2xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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YFP-Rac1

Plasmid

#11391

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 1 (RAC1 Human)

Tags

YFP

Expression

Mammalian

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

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AAV-CMVc-Cas9

Plasmid

#106431

Purpose

Expresses SpCas9 from the CMVc promoter in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

Cas9

Use

AAV and CRISPR

Tags

HA-NLS and NLS

Expression

Mammalian

Promoter

CMVc

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBigT

Plasmid

#21270

Purpose

Used for making transgenic mice. Contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence.

Depositor

Frank Costantini

Article

Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

Type

Empty backbone

Use

Mouse Targeting

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pWPI-IRES-Bla-Ak-ACE2-TMPRSS2

Plasmid

#154983

Purpose

Lentiviral vector expressing Human ACE2 and TMPRSS2

Depositor

Sonja Best

Insert

ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)

Use

Lentiviral

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-Cdk9

Plasmid

#28100

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO-RB1-shRNA19

Plasmid

#25640

Purpose

3rd generation lentiviral vector

Depositor

Todd Waldman

Article

Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

Insert

RB1-shRNA 19 (RB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pVT100U-mtGFP

Plasmid

#45054

Depositor

Benedikt Westermann

Article

Westermann et al Yeast. 2000 Nov;16(15):1421-7.

Insert

mtGFP

Expression

Yeast

Promoter

ADH

Available Since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGFP

Plasmid

#32748

Depositor

Jeanne Hardy

Article

Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

Insert

GFP

Tags

DEVDFQGPCNDSSDPLVVAASIIGILHLILWILDRL

Expression

Mammalian

Mutation

S65T

Promoter

CMV

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

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pGL2B -1538/+64

Plasmid

#24942

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge