We narrowed to 17,661 results for: URE

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bArr2-V5

Plasmid

#201494

Purpose

Expression of the beta-arrestin2 containing a N-terminal V5 tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

bArr2-V5 (ARRB2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-p38KTRmCerulean3

Plasmid

#59149

Purpose

ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (D1320A/D1709A)

Plasmid

#41585

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

D1320A/D1709A

Promoter

CMV

Available Since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only
eGFPbait-E2A-KalTA4-pA donor vector

Plasmid

#61069

Purpose

for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)

Depositor

Filippo Del Bene

Article

Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

Inserts

eGFPbait
KalTA4

Use

Zebrafish expression

Tags

E2A

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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NO- DSD Sensor 2

Plasmid

#199786

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

NO- DSD Sensor

Use

Synthetic Biology

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-P2A-PuroR

Plasmid

#196962

Purpose

Expresses PE2 with puromycin resistance

Depositor

Leopold Parts

Article

Koeppel et al Nat Biotechnol. 2023 Feb 16. doi: 10.103

Insert

PE2-P2A-Puro

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a Ov-iPGM

Plasmid

#180245

Purpose

Bacterial expression plasmid for production of recombinant Onchocerca volvulus iPGM His10

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

Onchocerca volvulus iPGM-10His

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a Ce-iPGM-bio

Plasmid

#162559

Purpose

Expresses C. elegans iPGM containing a C-terminal biotinylation site and His tag

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

C. elegans iPGM bio-6His (ipgm-1 Nematode)

Tags

6His tag, T7 tag, Thrombin cleavage site, and bio…

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a Bm-iPGM-bio

Plasmid

#162565

Purpose

Expresses B. malayi iPGM containing a C-terminal biotinylation site and His tag

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

B. malayi iPGM bio-6His

Tags

6His tag, T7 tag, biotinylation sequence, and thr…

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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TT lux sensor

Plasmid

#199792

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

TT lux Sensor

Use

Synthetic Biology

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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hCD12

Plasmid

#225541

Purpose

Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.

Depositor

David Christianson

Article

Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

Insert

Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)

Tags

His6-MBP tag with cleavable TEV site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pSL0828 (pQCascade_crRNA-4)

Plasmid

#130637

Purpose

Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLUEF1 Luc2-IRES-GFP

Plasmid

#224467

Purpose

Ef1a downstream of a ubiquitous chromatin opening element drives expression of Luciferase2 and IRES GFP

Depositor

Robert Kerbel

Article

Khan et al Cancer Res. 2025 Apr 14. doi: 10.1158/00

Insert

Luciferase

Use

Lentiviral

Promoter

EF1a

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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TNF-SBP-EGFP_puromycin

Plasmid

#65284

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_TP53BP2-1/1

Plasmid

#91503

Purpose

Protein expression and purification of human SH3 domain construct TP53BP2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TP53BP2-1/1 (TP53BP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-SpCas9-BlastR

Plasmid

#107190

Purpose

Confers constitutive expression of SpCas9

Depositor

Richard Sherwood

Article

Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

Insert

SpCas9 (NEWENTRY )

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial and Mammalian

Promoter

Chicken ß-actin

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAc5.1-V5-HisB-ObKHC-wt-HaloTag

Plasmid

#201551

Purpose

Expresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cells

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Kinesin-1 (partial)

Tags

HaloTag

Expression

Insect

Mutation

Codon-optimized for Drosophila expression

Promoter

Ac5

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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B2342_BTB-mCherry

Plasmid

#215095

Purpose

Expresses BCL6 BTB(1-129)-mCherry in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BCL6 BTB (BCL6 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHRi_1G4 hTCRβ-SNAP

Plasmid

#187604

Purpose

Lentiviral expression of hTCR beta(1G4), and SNAP for low expression

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

1G4 hTCR beta, and SNAP tag (TRB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mHSP

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo1-WT 6KQ

Plasmid

#17562

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 6KQ (Foxo1 Mouse)

Tags

Flag and Myc

Expression

Mammalian

Mutation

K242, K245, K259, K262, K271 and K291 were replac…

Promoter

CMV

Available Since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-BBS7

Plasmid

#218715

Purpose

Expresses N-terminally EGFP-tagged BBS7 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS7 (BBS7 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS-N-HA

Plasmid

#130913

Purpose

Expresses human cGAS N terminus(aa1-159)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGAS N terminus

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-VCPIP1

Plasmid

#85671

Purpose

Expression of VCPIP1 with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

VCPIP1 (VCPIP1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST15 hSlp4-a

Plasmid

#40046

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a (SYTL4 Human)

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pCS2+ Zf GFP-vclb

Plasmid

#185411

Purpose

GFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

vinculin b (vclb Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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AA304

Plasmid

#216072

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v1 [Sa]; terminator_v1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Kmt5cFL-CatMut

Plasmid

#235590

Purpose

Dox-inducible expression of control catalytic-mutant Kmt5c CD fused with scFV

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Kmt5c (Kmt5c Mouse)

Use

CRISPR

Mutation

NHDC182-185AAAG

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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EYFP-Mito-7

Plasmid

#56596

Purpose

Localization: Mitochondria, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Mito (COX8A Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pC-HYG-YR

Plasmid

#61765

Purpose

Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).

Depositor

Ken Haynes

Article

Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

Inserts

Hygromycin
2 micron origin or replication and URA3 gene for S. cerevisiae

Use

Ternary vector for agrobacterium mediated transfo…

Promoter

trpC promoter

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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His-MBP-NRBF2

Plasmid

#99330

Purpose

Mammalian expression of NRBF2, which associates with the PI3KC3-C1 complex

Depositor

James Hurley

Article

Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

Insert

Nuclear receptor-binding factor 2 (NRBF2 Human)

Tags

His-MBP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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