19,660 results

1. PCMV-intron myc Rab2 S20N

   Plasmid

   #46780

   Purpose

   expression of S20N Rab2a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   RAB2 (RAB2A Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S20N dominant negative

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pmCherry-Talin

   Plasmid

   #80026

   Purpose

   Mammalian expression of mCherry-Talin

   Depositor

   Chinten James Lim

   Article

   Lee et al Mol Biol Cell. 2013 May;24(9):1354-62. d

   Insert

   Talin
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-VP4SA11

   Plasmid

   #89165

   Purpose

   Express Rotavirus SA11 VP4 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 VP4
   

   Expression

   Mammalian

   Promoter

   T7 promotor

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. CRY2-GFP-homer1c

   Plasmid

   #89442

   Purpose

   Localizes CRY2 to excitatory synapses

   Depositor

   Matthew Kennedy

   Article

   Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi

   Insert

   CRY2-GFP-homer1c
   

   Expression

   Mammalian

   Mutation

   Contains Full length CRY2

   Promoter

   CAG

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCM184-ParB2::eGFP

   Plasmid

   #87252

   Purpose

   Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   ParB2
   

   Tags

   EGFP

   Expression

   Yeast

   Promoter

   pBAD/tetO

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-Cas9-T2A-TdT

   Plasmid

   #126424

   Purpose

   Expresses Cas9-T2A-TdT in mammalian cells. (pTBL209)

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-T2A-TdT (DNTT Human, Streptococcus pyogenes)
   

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB554

   Plasmid

   #74884

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDG462

   Plasmid

   #100903

   Purpose

   SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9 Nickase
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A mutant converts to Nickase

   Promoter

   CBh and U6

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB_iMYOD1_P2A_GFP_Puro

   Plasmid

   #168804

   Purpose

   PiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expression

   Depositor

   Prashant Mali

   Article

   Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

   Insert

   MYOD1 (MYOD1 Human, Synthetic)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWS175

    Plasmid

    #90962

    Purpose

    Cas9 gap repair vector - HygR

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFC333

    Plasmid

    #87844

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    ble (bleomycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57-RFP-P2A-MCS

    Plasmid

    #78933

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Expression

    Mammalian

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-IC2-FL

    Plasmid

    #51409

    Purpose

    expression of EGFP-tagged full-length dynein intermediate chain IC2C in mammalian cells

    Depositor

    Trina Schroer

    Article

    King et al Mol Biol Cell. 2003 Dec;14(12):5089-97.

    Insert

    dynein intermediate chain IC2C (Dync1i2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-IKZF1-V2-V5

    Plasmid

    #107390

    Purpose

    Mammalian expression of IKZF1-V2-V5

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF1-V2 (IKZF1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-6xGLY-His-tag-hphMX4

    Plasmid

    #20764

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Expression

    Yeast

    Available Since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-6xHis-(pos36)GFP

    Plasmid

    #62937

    Purpose

    Expression of 6xHis-(+36)GFP in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (pos36)GFP
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBbA5c-MevT(CO)-MBIS(CO, ispA)

    Plasmid

    #35151

    Depositor

    Jay Keasling, Taek Soon Lee

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    R364S in Mevalonate Kinase

    Promoter

    LacUV5,

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-EF1a-MICB*005-IRES-ZsGreen

    Plasmid

    #114008

    Purpose

    Induces expression of human MICB allele 005 cDNA

    Depositor

    Kai Wucherpfennig

    Article

    Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

    Insert

    MICB*005 (MICB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVE13300

    Plasmid

    #137715

    Purpose

    Expresses impLbCas12a in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    impLbCas12a
    

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWPI-IRES-Puro-Ak-ACE2-TMPRSS2

    Plasmid

    #154987

    Purpose

    Lentiviral vector expressing Human ACE2 and TMPRSS2

    Depositor

    Sonja Best

    Insert

    ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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