20,361 results

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Publication

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLentiPGK Hygro DEST H2B-mRuby2

Plasmid

#90236

Purpose

Lentiviral vector to express H2B-mRuby2 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Publication

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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SunTagng22aa

Plasmid

#106438

Purpose

Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.

Depositor

Steven Jacobsen

Publication

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CKIIa-stGtACR2-FusionRed

Plasmid

#105669

Purpose

Expresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoter

Depositor

Ofer Yizhar

Publication

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Has service

AAV1 and AAV Retrograde

Insert

stGtACR2

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Promoter

CaMKIIa

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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MBP-hnRNPA2_FL_WT

Plasmid

#98662

Purpose

Expresses MBP-tagged full length hnRNPA2

Depositor

Nicolas Fawzi

Publication

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis-MBP

Expression

Bacterial

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pXPR_206

Plasmid

#96920

Purpose

for SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffold

Depositor

John Doench, David Root

Publication

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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EGFP-Rab8a-wt

Plasmid

#86075

Purpose

A mammalian expression plasmid encoding Rab8a wild type with N-terminus EGFP.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hRab8A (RAB8A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pT7-NSP3SA11

Plasmid

#89170

Purpose

Express Rotavirus SA11 NSP3 in mammalian cells

Depositor

Takeshi Kobayashi

Publication

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rotavirus SA11 NSP3

Expression

Mammalian

Promoter

T7 promotor

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET-BiFC

Plasmid

#87856

Purpose

pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.

Depositor

Dan Mulvihill

Publication

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Inserts

Encodes for MCS & C-term half of mVenus
Encodes for MCS & N-term half of mVenus

Tags

BiFC C-terminal fragment

Expression

Bacterial

Promoter

T7

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDNA3_UnaG-Flag-Sec61B

Plasmid

#83413

Purpose

Expressed N-term tagged UnaG on Sec61B

Depositor

Hyun-Woo Rhee

Publication

Park et al ACS Chem Biol. 2016 May 27.

Insert

UnaG-Sec61B (SEC61B Human, Synthetic)

Expression

Mammalian

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBK-VRER-BE3

Plasmid

#85173

Purpose

Expresses VRER-BE3 in mammalian cells

Depositor

David Liu

Publication

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VRER-BE3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pBK-YE1-BE3

Plasmid

#85174

Purpose

Expresses YE1-BE3 in mammalian cells

Depositor

David Liu

Publication

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YE1-BE3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-RAM-d2TTA::TRE-ChR2-WPREpA

Plasmid

#84471

Purpose

pAAV-RAM-ChR2

Depositor

Yingxi Lin

Publication

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Has service

AAV8

Insert

Channelrhodopsin 2

Use

AAV

Tags

EYFP

Mutation

H134R

Promoter

TRE

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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mKO2-SLBP(18-126)

Plasmid

#83914

Purpose

Fluorescent probe for S/G2 cell cycle transition

Depositor

Michael Lin

Publication

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mKO2-SLBP(18-126) (SLBP Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGWB504

Plasmid

#74846

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CRY2(L348F)-CreN

Plasmid

#75368

Purpose

Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase

Depositor

Chandra Tucker

Publication

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRY2 (CRY2 Mustard Weed)

Tags

Cre recombinase, residues 19-104

Expression

Mammalian

Mutation

L348F

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCDH-puro-CMV-RC3AI

Plasmid

#78908

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Publication

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

RC3AI

Use

Lentiviral

Tags

HA

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-RIPK3

Plasmid

#78815

Purpose

To overexpress RIPK3 in Mammalian Cells

Depositor

Jaewhan Song

Publication

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RIPK3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJMK074: CMV QuasAr2-TS-GCaMP6f

Plasmid

#72303

Purpose

Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

Depositor

Adam Cohen

Publication

Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

Insert

CaViar

Use

Lentiviral

Tags

TS

Expression

Mammalian

Promoter

CMV

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJKR-H-tetR

Plasmid

#62561

Purpose

Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Publication

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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